Results of a comparative study of <i>Nigella Sativa</i> L. Seeds oils composition

Abstract

This article presents results of the chemical composition study of the seeds oils lipid complex of Nigella Sativa L. grown under various geographic conditions. The task of the comprehensive study of the chemical composition of the plant and its individual parts remains relevant due to the wide spectrum of its pharmacological activity.The aim of this work is a comparative study of the fatty acid composition, a non-saponifiable fraction and the composition of essential oils of Nigella Sativa L. seeds grown in different regions of the world.Materials and methods. The combination of chromatography-mass spectrometry and 1H-NMR spectroscopy methods made it possible to study the qualitative and quantitative composition of Nigella Sativa L. lipid complex seeds. All the experiments were carried out in accordance with the requirements of the State Pharmacopoeia, 14th Ed, given in the corresponding general pharmacopeial monographs.Results. Profiles have been established and the content of fatty acids, sterines, triterpene alcohols, essential oils and thymoquinone found out in the lipid complex, has been estimated. The saponifiable portion of the complex is represented by triglycerides (81.7–95.3%), di(3.9–15.2%) and monoglycerides (0.7–4.1%). They mainly contain linoleic (55.8–60.6%), oleic (21.8–24.6%), palmitic (10.0–12.8%), stearic (2.4–3.2 %) and cis-11.14-eicosadiene (2.3–2.6%) acids. In the lipid complex, the contents of sterines and triterpene alcohols were 0.4–0.7%; up to 70% of the fraction was represented by β-sitosterol (22.5–29.2%), cycloartenol (20.1–36.6%) and 24 methylenecycloartanol (9.5–19.9%). In the trace amounts (up to 1.0%), cholesterol has been detected in all the samples. In the lipid complexes, the content of thymoquinone ranged from 0.7 to 2.6%.Conclusion. A comparative study of the seeds lipid complex of Nigella Sativa L. grown under various geographic conditions, has been carried out. The marker compounds as well as their content standards for determining the authenticity of raw materials (thymoquinone, para-cimen, cis-11.14-eicosadienic acid), have been identified.

Study of the Chemical Composition of Carica papaya L. Seed Oils of Various Geographic Origins

“…The GC-MS analysis was performed to confirm the fatty acid composition of the extracted seed oil by reanalyzing the FAMEs mixtures of papaya seed oil samples. The components of the unsaponifiable fraction were identified by matching their recorded mass spectra with the standard mass spectra from the National Institute of Standards and Technology (NIST'17) libraries, provided by the software of the GC-MS system (Xcalibur ver 1.4 SR2), as well as the literature data and the results of our previous research [28,35]. The results were confirmed by analyzing the mass spectra of the produced compounds and also by comparing their retention indices with those indices reported in the literature [29,35].…”

“…NMR spectroscopy and gas chromatography-mass spectrometry (GC-MS) have undoubted advantages and allow us to characterize different multicomponent systems (e.g., edible oils) at the component and fragment levels [24,28]. Therefore, these two methods were chosen to comparatively study the composition of papaya seed oils from different geographical regions.…”

Study of the Chemical Composition of Carica Papaya L. Seed Oils of Various Geographic Origin

“…NMR spectroscopy and gas chromatography -mass spectrometry (GC-MS) have undoubted advantages and allow to characterize different multicomponent systems (e.g. edible oils) on component and fragmentary levels [25,27]. That was a reason to choose these two methods for the comparative study the composition of papaya seed oils from different geographical regions.…”