Restriction Map of the Serratia entomophila Plasmid pADAP Carrying Virulence Factors for Costelytra zealandica

Hurst, Mark R. H.; Glare, Travis R.

doi:10.1006/plas.2001.1551

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…1 and Table 2). Concurrently, peripheral sequencing data of various pADAP subclones had tentatively located the regions of pADAP replica- tion and conjugation, a fimbrial operon, and a region of DNA of unknown translated identity (24). The last region, which encompassed one of the three large HindIII fragments designated pMH52 (Fig.…”

Section: Resultsmentioning

confidence: 94%

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CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

2004

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Serratia entomophila and Serratia proteamaculans (Enterobacteriaceae) cause amber disease in the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. Larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. A 155-kb plasmid, pADAP, carries the genes sepA, sepB, and sepC, which are essential for production of amber disease symptoms. Transposon insertions in any of the sep genes in pADAP abolish gut clearance but not cessation of feeding, indicating the presence of an antifeeding gene(s) elsewhere on pADAP. Based on deletion analysis of pADAP and subsequent sequence data, a 47-kb clone was constructed, which when placed in either an Escherichia coli or a Serratia background exerted strong antifeeding activity and often led to rapid death of the infected grass grub larvae. Sequence data show that the antifeeding component is part of a large gene cluster that may form a defective prophage and that six potential members of this prophage are present in Photorhabdus luminescens subsp. laumondii TTO1, a species which also has sep gene homologues.

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Section: Resultsmentioning

confidence: 94%

“…Analysis of sequence data had identified a large area of unknown translated identity flanked by the sep virulence-associated region and a transposon element identified in the previously mapped clone pUH5.4 (24) (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

2004

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“…Three pADAP subclones were used as probes (Table 1; Fig. 1): (i) pAC⌬8, which encompasses the sepABC virulence-associated region; (ii) pUB15, which encodes 16 open reading frames (ORFs) with high similarity to components of the type IV pilus and other conjugative transfer components of the E. coli plasmid R64 (19); and (iii) pUH1.8, the cloned DNA of which has translated similarity to two proteins involved in plasmid replication and partitioning and is the predicted replication region of pADAP (unpublished data) from several members of the Enterobacteriaceae (17). Gel-eluted gene-specific probes for sepA, sepB, or sepC were derived from the plasmid clones paraA, paraB, and paraC, respectively ( Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Transposon insertions in sepA, sepB, or sepC completely abolished both gut clearance and cessation of feeding (18). Through the mapping of pADAP and the use of sequences derived from the peripheral region of pADAP subclones, the locations of putative insertion elements and genes involved in the replication and conjugative transfer of pADAP were also determined (17). More recently, an additional pADAP gene cluster encoding antifeeding determinants was described (20).…”

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Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

Dodd

Hurst

Glare

et al. 2006

Appl Environ Microbiol

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Some strains of Serratia entomophila and S. proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. Sequence analysis suggests that the sepABC gene cluster may be part of a horizontally mobile region. This study presents evidence for the putative mobility of the sep genes of pADAP. Southern blot analysis showed that orthologues of the sep genes reside on plasmids within S. entomophila, S. liquefaciens, S. proteamaculans, and a plasmid from Yersinia frederiksenii. Three plasmids hybridized to the pADAP sep virulenceassociated region but not the pADAP replication and conjugation regions. Subsequent DNA sequence analysis of the Y. frederiksenii sep-like genes, designated tcYF1 and tcYF2, showed that they had 88% and 87% DNA identity to sepA and sepB, respectively. These results indicate that the sep genes are part of a discrete horizontally mobile region.

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“…The molecular mechanism of pathogenicity is unclear. However, the pathogenic determinants seem to be coded in a 155-kb plasmid (16). Sequencing data and genetic evidence have shown that the antifeeding component is part of a large gene cluster that forms a defective prophage (17) containing three DNA regions, (i) the amb2 locus, (ii) the intact lysis cassette, and (iii) the antifeeding prophage cluster.…”

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Identification of a Putative Mexican Strain ofSerratia entomophilaPathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera)

Nuñez-Valdez

Calderón

Aranda

et al. 2008

Appl Environ Microbiol

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The larvae of scarab beetles, known as "white grubs" and belonging to the genera Phyllophaga and Anomala (Coleoptera: Scarabaeidae), are regarded as soil-dwelling pests in Mexico. During a survey conducted to find pathogenic bacteria with the potential to control scarab larvae, a native Serratia sp. (strain Mor4.1) was isolated from a dead third-instar Phyllophaga blanchardi larva collected from a cornfield in Tres Marías, Morelos, Mexico. Oral bioassays using healthy P. blanchardi larvae fed with the Mor4.1 isolate showed that this strain was able to cause an antifeeding effect and a significant loss of weight. Mortality was observed for P. blanchardi, P. trichodes, and P. obsoleta in a multidose experiment. The Mor4.1 isolate also caused 100% mortality 24 h after intracoelomic inoculation of the larvae of P. blanchardi, P. ravida, Anomala donovani and the lepidopteran insect Manduca sexta. Oral and injection bioassays were performed with concentrated culture broths of the Mor4.1 isolate to search for disease symptoms and mortality caused by extracellular proteins. The results have shown that Mor4.1 broths produce significant antifeeding effects and mortality. Mor4.1 broths treated with proteinase K lost the ability to cause disease symptoms and mortality, in both the oral and the injection bioassays, suggesting the involvement of toxic proteins in the disease. The Mor4.1 isolate was identified as a putative Serratia entomophila Mor4.1 strain based on numerical taxonomy and phylogenetic analyses done with the 16S rRNA gene sequence. The potential of S. entomophila Mor4.1 and its toxins to be used in an integrated pest management program is discussed.

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Restriction Map of the Serratia entomophila Plasmid pADAP Carrying Virulence Factors for Costelytra zealandica

Cited by 9 publications

References 20 publications

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

Identification of a Putative Mexican Strain ofSerratia entomophilaPathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera)

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