Cloning<i>Serratia entomophila</i>Antifeeding Genes—a Putative Defective Prophage Active against the Grass Grub<i>Costelytra zealandica</i>

Hurst, Mark R. H.; Glare, Travis R.; Jackson, Trevor A.

doi:10.1128/jb.186.15.5116-5128.2004

Cited by 123 publications

(140 citation statements)

References 51 publications

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“…one locus, comprising 18 genes, is required for cessation of feeding. Sixteen of these genes encode proteins that could form a defective prophage (called Afp for antifeeding prophage) 42 . Expression of the afp genes in E. coli allows the synthesis of particles that resemble phage tail-like bacteriocins 43 .…”

Section: Pathogenicmentioning

confidence: 99%

Bacterial strategies to overcome insect defences

2008

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| Recent genetic and molecular analyses have revealed how several strategies enable bacteria to persist and overcome insect immune defences. Genetic and genomic tools that can be used with Drosophila melanogaster have enabled the characterization of the pathways that are used by insects to detect bacterial invaders and combat infection. Conservation of bacterial virulence factors and insect immune repertoires indicates that there are common strategies of host invasion and pathogen eradication. Long-term interactions of bacteria with insects might ensure efficient dissemination of pathogens to other hosts, including humans. R E V I E W S302 | APRIL 2008 | voLUME 6 www.nature.com/reviews/micro

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Section: Pathogenicmentioning

confidence: 99%

Bacterial strategies to overcome insect defences

2008

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“…This phenomenon is also observed in the interaction between Serratia entomophila and the grass grub Costelytra zealandica or between Yersinia pestis and the flea. The processes used to effect food blockage seem to be different in the two systems; Y. pestis relies on phospholipase synthesis and biofilm formation 36,37 whereas the mechanism used by S. entomophila remains unknown 38 . Genes responsible for the anti-feeding determinants of S. entomophila have a prophage origin and no related genes were identified in the genome of P. entomophila.…”

Section: Metabolism Transport and Regulationmentioning

confidence: 99%

Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila

et al. 2006

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Pseudomonas entomophila is an entomopathogenic bacterium that, upon ingestion, kills Drosophila melanogaster as well as insects from different orders. The complete sequence of the 5.9-Mb genome was determined and compared to the sequenced genomes of four Pseudomonas species. P. entomophila possesses most of the catabolic genes of the closely related strain P. putida KT2440, revealing its metabolically versatile properties and its soil lifestyle. Several features that probably contribute to its entomopathogenic properties were disclosed. Unexpectedly for an animal pathogen, P. entomophila is devoid of a type III secretion system and associated toxins but rather relies on a number of potential virulence factors such as insecticidal toxins, proteases, putative hemolysins, hydrogen cyanide and novel secondary metabolites to infect and kill insects. Genome-wide random mutagenesis revealed the major role of the two-component system GacS/GacA that regulates most of the potential virulence factors identified.

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“…To further elucidate the evolutionary relationships between contractile tails and the T6SS, we analyzed operons encoding T6SSs and PVCs. PVCs are a separate class of contractile tail-related apparatuses that have been implicated in killing insect larva and tubeworm morphogenesis (17)(18)(19). We analyzed these because they are more closely related to phage tails than the T6SS, yet display features of a secretion system (3,17).…”

Section: Defining the Broadly Conserved Components Of The Simple Contmentioning

confidence: 99%

Baseplate assembly of phage Mu: Defining the conserved core components of contractile-tailed phages and related bacterial systems

Buttner

Maxwell

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Contractile phage tails are powerful cell puncturing nanomachines that have been co-opted by bacteria for self-defense against both bacteria and eukaryotic cells. The tail of phage T4 has long served as the paradigm for understanding contractile tail-like systems despite its greater complexity compared with other contractile-tailed phages. Here, we present a detailed investigation of the assembly of a "simple" contractile-tailed phage baseplate, that of Escherichia coli phage Mu. By coexpressing various combinations of putative Mu baseplate proteins, we defined the required components of this baseplate and delineated its assembly pathway. We show that the Mu baseplate is constructed through the independent assembly of wedges that are organized around a central hub complex. The Mu wedges are comprised of only three protein subunits rather than the seven found in the equivalent structure in T4. Through extensive bioinformatic analyses, we found that homologs of the essential components of the Mu baseplate can be identified in the majority of contractile-tailed phages and prophages. No T4-like prophages were identified. The conserved simple baseplate components were also found in contractile tailderived bacterial apparatuses, such as type VI secretion systems, Photorhabdus virulence cassettes, and R-type tailocins. Our work highlights the evolutionary connections and similarities in the biochemical behavior of phage Mu wedge components and the TssF and TssG proteins of the type VI secretion system. In addition, we demonstrate the importance of the Mu baseplate as a model system for understanding bacterial phage tail-derived systems.baseplate | assembly | contractile tail | phage Mu

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CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

Cited by 123 publications

References 51 publications

Bacterial strategies to overcome insect defences

Bacterial strategies to overcome insect defences

Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila

Baseplate assembly of phage Mu: Defining the conserved core components of contractile-tailed phages and related bacterial systems

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