Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila

Vodovar, Nicolas; Vallenet, David; Cruveiller, Stéphane; Rouy, Zoé; Barbe, Valérie; Acosta, Carlos Alberto Palacio; Cattolico, Laurence; Jubin, Claire; Lajus, Aurélie; Ségurens, Béatrice; Vacherie, Benoît; Wincker, Patrick; Weissenbach, Jean; Lemaître, Bruno; Médigue, Claudine; Boccard, Frédéric

doi:10.1038/nbt1212

Cited by 234 publications

(218 citation statements)

References 47 publications

(50 reference statements)

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“…In several cases, constitutive expression of the Imd pathway of AMP genes results in host protection 49,50,80,81 , which reveals the importance of the race between the establishment of resistant bacterial populations and the activation of the immune response. Furthermore, it demonstrates that the host immune response can be effective against invaders.…”

Section: Discussionmentioning

confidence: 99%

“…P. entomophila might protect itself from immune effectors such as AMP, or it might degrade these effectors. Among the various putative virulence factors that are encoded in the P. entomophila genome 50 , the secreted zinc metalloprotease AprA has a pivotal role in the defence of P. entomophila against the host gut epithelial immune response 49 . Not only is an aprA mutant slightly less virulent, it is also less able to persist in D. melanogaster than wild-type P. entomophila, and is more sensitive to Imd-mediated defences.…”

Section: Immune Defences In the Gutmentioning

confidence: 99%

“…In addition to protein factors, several entomopathogens produce toxic secondary metabolites 50,71 that might either harm the host or out-compete other microorganisms 64 .…”

Section: Box 3 | Heritable Microorganismsmentioning

confidence: 99%

“…Global regulators play an important role in virulence towards insects: the GacS/GacA two-component system controls the P. entomophila infectious process 50 , PhoP/PhoQ is the master regulator of virulence in P. luminescens 72 , Lrp regulates both mutualism and pathogenicity in Xenorhabus 73 and PlcR activates the transcription of genes required for virulence in B. thuringiensis 74,75 . Another striking example is found in E. c. carotovora 15, where the evf gene is under the control of Hor, a regulator of genes that are involved in plant pathogenesis 36 .…”

Section: Box 3 | Heritable Microorganismsmentioning

confidence: 99%

“…ability to colonize and persist in insect species might have resulted from horizontal gene transfer (HGT) of plasmid-borne persistence genes in S. entomophila (afp-encoding genes) and in M. ulcerans (mycolactone biosynthetic genes). A role for HGT in the evolution of entomopathogens is also revealed by the distribution of genes that encode similar toxins in unrelated genera 50,77,78 , which suggests that the insect gut constitutes an environment that is favourable for gene transfer 79 .…”

Section: Box 4 | Bacillus Thuringiensis and Other Bacillus Sppmentioning

confidence: 99%

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Bacterial strategies to overcome insect defences

2008

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| Recent genetic and molecular analyses have revealed how several strategies enable bacteria to persist and overcome insect immune defences. Genetic and genomic tools that can be used with Drosophila melanogaster have enabled the characterization of the pathways that are used by insects to detect bacterial invaders and combat infection. Conservation of bacterial virulence factors and insect immune repertoires indicates that there are common strategies of host invasion and pathogen eradication. Long-term interactions of bacteria with insects might ensure efficient dissemination of pathogens to other hosts, including humans. R E V I E W S302 | APRIL 2008 | voLUME 6 www.nature.com/reviews/micro

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Section: Discussionmentioning

confidence: 99%

Section: Immune Defences In the Gutmentioning

confidence: 99%

“…In addition to protein factors, several entomopathogens produce toxic secondary metabolites 50,71 that might either harm the host or out-compete other microorganisms 64 .…”

Section: Box 3 | Heritable Microorganismsmentioning

confidence: 99%

Section: Box 3 | Heritable Microorganismsmentioning

confidence: 99%

Section: Box 4 | Bacillus Thuringiensis and Other Bacillus Sppmentioning

confidence: 99%

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Bacterial strategies to overcome insect defences

2008

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Hemophore Has A

Izadi-Pruneyre¹,

Wolff²,

Delepierre³

et al. 2004

Handbook of Metalloproteins

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To satisfy their iron needs, several gram‐negative bacteria use a heme uptake system that relies on the secretion in the extracellular medium of heme‐binding proteins called HasA hemophores . These proteins scavenge heme from the host, either free or hemoprotein‐bound, and shuttle it to a specific outer membrane transporter, HasR, whereby it is internalized by a TonB‐dependent process and then used as an iron source. HasA hemophores form a family of highly conserved 19–20 kDa proteins without either sequential or structural similarities to other known proteins. The HasA hemophore from Serratia marcescens , the most deeply studied, is described here with a special focus on its heme binding site and its unusual pair of axial ligands, a histidine and a tyrosine. The spectroscopic properties and the structures of the heme loaded and unloaded hemophore, free or complexed to the HasR transporter are presented. The mechanisms of heme capture by the hemophore and heme transfer to HasR are discussed.

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