Responses of Selected Indonesian Rice Varieties under Excess Iron Condition in Media Culture at Seedling Stage

Nugraha, Yudhistira; Rumanti, Indrastuti Apri; Guswara, Agus; Ardie, Sintho Wahyuning; Suwarno, Suwarno; Ghulammahdi, Munif; Aswidinnoor, Hajrial

doi:10.21082/jpptp.v35n3.2016.p181-190

Cited by 6 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result showed that iron content in the root were higher than the shoot in both green house and field. This result was similar to those reported that one of adaptive mechanism was aim to exclude iron into shoot and hold it in rhizosphere through capability of roots oxidizing iron before being absorbed [8,10,23,25,26]. The ANOVA in field condition showed that the effect of genotype was an insignificant different on iron content in root and shoot, but it was significantly different in iron uptake.…”

Section: Iron Content and Iron Uptakesupporting

confidence: 89%

“…Tolerance-typed to iron toxicity can be categorized into two types namely, exclusion and inclusion tolerance-type. This identification can be done simply by checking the presence of Fe 2+ in the shoots and root of rice plant [21][22][23]. Shoot and root iron content, and iron uptake was analyzed using wet destruction with HNO3 and HClO4 (methods).…”

Section: Methodsmentioning

confidence: 99%

“…Iron toxicity interferes metabolic processes and cause damage the rice plants organs, which is marked by leaf bronzing symptoms, delayed of flowering, inhibited assimilate processes, dwarfed of plant posture, less developed of root system, decayed, roughed and shorted of stems and leaves [6,7] Rice plant can develop a mechanism of avoidance or tolerance both morphologically and physiologically to survive on soil conditions with high iron solubility. Four types of plant adaptation to iron toxicity [8], such as 1) Avoidance inclusion, roots remove iron to avoid damage of shoot tissue by oxidation in the zone of rhizosphere or through mechanism of root selectivity to ion [9,10], or increased pH of soil solution with OH efflux to decrease Fe 2+ activity [11]; 2) Avoidance inclusion, iron absorbed by roots but damage of plant tissues may be avoided by compensation such as leaves that are not actively photosynthesized [12], or stored in cell vacuoles, iron storage proteins [13,14]; 3) Tolerant inclusion, plant is able to tolerate iron into the shoot tissue by detoxifying enzymatically effects of iron toxicity [14,15]; 4) Membrane selectivity through transporter protein Iron Regulated Transporter [16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Response of Indonesian rice varieties to iron toxicity under field and green house condition

Sitaresmi¹,

Dewi

Rumanti³

et al. 2021

E3S Web Conf.

Self Cite

View full text Add to dashboard Cite

Excess of reduced iron (Fe2+) will affect the plant growth of rice when it is grown under waterlogged condition. Some rice genotypes have been identified tolerance to iron toxicity with different mechanism-type of tolerance. This study aims to identify the response of some rice genotypes to iron toxicity, and to evaluate their consistency in the greenhouse screening method and the field condition. The experiments were conducted in iron toxicity hotspot area, Banyuasin (South Sumatera) and the control as irrigated rice field was in Sukamandi (West Java). The green house experiment of Fe toxicity was done using 400 ppm of Iron with Yoshida Agar Solution method. The response of rice plant to iron toxicity was observed on leaf bronzing score (LBS), iron content and uptake, biomass, yield and yield components and stress tolerant index (STI). We found that there was not clear relationship between the iron content with the leaf bronzing symptom depending on type of tolerant while the stress tolerance index was corelated with the yield. There were two varieties was identified as tolerant, namely Cilamaya Muncul as includer tolerant genotype and Awan Kuning as the excluder tolerant genotype. The information of the distinct response those rice germplasms can be used for recommendation for further study and development of rice tolerant to iron toxicity condition.

show abstract

Section: Iron Content and Iron Uptakesupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Response of Indonesian rice varieties to iron toxicity under field and green house condition

Sitaresmi¹,

Dewi

Rumanti³

et al. 2021

E3S Web Conf.

Self Cite

View full text Add to dashboard Cite

show abstract

“…IR64 and Inpara 5 as sensitive varieties to Fe toxicity stress, 35,36 Inpara 2 is categorized as a moderate tolerant variety to Fe toxicity stress, 36 while Pokkali is categorized as a tolerant variety. 17,[35][36][37] Fe toxicity stress disturbs metabolisms in plant, which is manifested as the formation of leaf bronzing that causes a reduction in plant growth and yield. 6,27,36,38 The bronzing symptom begins at the tips of the oldest leaves and spreads throughout the leaves.…”

Section: Discussionmentioning

confidence: 99%

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Turhadi

Hamim

Ghulamahdi

et al. 2019

Plant Signaling & Behavior

View full text Add to dashboard Cite

Iron toxicity stress causes physiological and metabolic changes in rice and other plants. To date, there is little information about the metabolite profile of rice under Fe toxicity conditions. In fact, metabolite has a contribution to the physiological condition of plants. Plant metabolomics is a study of low-molecular weight metabolites in plants under certain conditions. The objective of the research was to investigate physiological and metabolic changes in rice under Fe toxicity stress. Two-week-old seedlings of four rice varieties with various Fe toxicity tolerance levels were stressed hydroponically with 400 ppm FeSO 4. 7H 2 O for 10 d. Numerous physiological characters were observed and untargeted metabolomic analysis was carried out using gas chromatography-mass spectrophotometry (GC-MS). The results showed Fe toxicity induced physiological and metabolite variation in rice. By comparing the metabolites synthesized in Fe toxicity-stressed plants with control plants, it showed that elaidic acid, linoleic acid, and linolenic acid could be as metabolite marker candidates for rice response to Fe toxicity stress. When plants exposed to Fe toxicity stress, elaidic acid increased, whereas linoleic-and linolenic acid decreased. The alteration of fatty acid composition in the root and shoot suggests the alteration of metabolites is one of the tolerance strategies of rice to Fe toxicity stress. This finding offers an insight about the tolerance strategies of rice under Fe toxicity stress related to the maintenance process of the cell membranes during this stress. The genes underlying biosynthesis of the fatty acid could be a target of future research on responsible genes for Fe toxicity tolerance in rice.

show abstract

“…The local rice cultivars consisted of 17 non-glutinous and six glutinous cultivars. An iron sensitive rice variety IR64 (Nugraha et al 2016;Mackill and Khush 2018); and tolerant variety Mekongga (Suprihatno et al, 2009;Nurhasanah 2017) were also included in this study.…”

Section: Plant Materialsmentioning

confidence: 99%

The response of East Kalimantan, Indonesia local rice cultivars against iron stress

2018

View full text Add to dashboard Cite

Nurhasanah, lestari HS, Sunaryo W. 2019. The response of East Kalimantan, Indonesia local rice cultivars against iron stress. Biodiversitas 20: 273-282. Iron (Fe) toxicity is one of the most problematic metal elements in acidic soil. Besides being as an essential micronutrient, an excessive iron can cause mineral and nutrients absorption disorder which leads to disruption of plant metabolism and cell development. Reduction of plant growth and yield will be the further consequences of the excessive soil iron content. This study aimed to evaluate the response of East Kalimantan local rice cultivars and to screen rice genotypes tolerant to iron stress. Twenty-five rice genotypes were used in this study, consisted of twenty-three local rice cultivars of East Kalimantan and two control of iron sensitive (IR64) and tolerant (Mekongga) varieties. Uniform sprouts (3 days old) having 1-1.5 cm root length were used for iron stress experiment. The seedlings were grown in nutrient solution using hydroponic system in an aerobic condition. The seedlings were treated for one week in iron stress condition by adding an extra iron source of 100 and 200 ppm FeSO4.7H2O (pH 4.0). The seedlings grown in the nutrient solution without an extra iron treatment at normal acidity growth condition (pH 5.8) were used as the control. The growth responses were observed from root, shoot, and biomass of the plants. The tolerance index of the plant growth characters was calculated to classify the rice genotypes into tolerant, moderate, and sensitive to iron stress. The results showed that 100 and 200 ppm of FeSO4.7H2O treatments inhibited the root and shoot growth and also reduced the plant biomass. The plant growth reduction was in parallel with the increase of iron concentration. There was a significant differential response of East Kalimantan local rice genotypes to iron stress treatment. Some genotypes showed an extreme reduction of plant growth, whereas several genotypes had an increased growth under stressed situation. In the contrary, the sensitive genotype IR 64 was consistently sensitive based on the tolerance index of the root, shoot, and plant biomass characters. Among all growth parameters, the most selective character for iron toxicity screening was maximal root length character. This character caused the most severe symptoms for most of the genotypes. Two local rice genotypes, Bentian and Bogor Hitam, were consistently tolerant based on the maximal root growth, total root growth, shoot length and plant biomass.

show abstract

Responses of Selected Indonesian Rice Varieties under Excess Iron Condition in Media Culture at Seedling Stage

Cited by 6 publications

References 24 publications

Response of Indonesian rice varieties to iron toxicity under field and green house condition

Response of Indonesian rice varieties to iron toxicity under field and green house condition

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

The response of East Kalimantan, Indonesia local rice cultivars against iron stress

Contact Info

Product

Resources

About