Response Surface Optimized Ultrasonic-Assisted Extraction of Quercetin and Isolation of Phenolic Compounds From<i>Hypericum perforatum</i>L. by Column Chromatography

Aybastıer, Önder; Şahin, Saliha; Demir, Cevdet

doi:10.1080/01496395.2012.760603

Cited by 25 publications

(9 citation statements)

References 31 publications

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“…The optimum conditions were determined as follows: 67 °C, 67 min, 77% MeOH, HCl concentration 1.2 m . The method was validated by experimental confirmation of the predicted quercetin content in the extract …”

Section: Sample Preparationmentioning

confidence: 99%

“…The optimum conditions were determined as follows: 67°C, 67 min, 77% MeOH, HCl concentration 1.2 M. The method was validated by experimental confirmation of the predicted quercetin content in the extract. [26] In case of in vivo studies, sample preparation of biological samples usually includes solvent extraction from blood plasma to enrich the analytes. For hyperforin, apolar extracting solvents, such as n-hexane: EtOAc 9 : 1 to 7 : 3, are used.…”

Section: Sample Preparationmentioning

confidence: 99%

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Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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Objectives The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions TLC and HPTLC are basic methods in the routine analysis, whereas HPLC‐DAD is the most widely applied method for quantitative analysis due to its versatility. LC‐MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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“…The FC method relies on the transfer of electrons from phenolic compounds and other reducing compounds to molybdenum. The FC method was performed according to the literature (Aybastıer et al, 2013). Briefly, the extract of chestnut bee pollen (100 µL), 1.9 mL of distilled water and 2.5 mL of Lowry C solution were taken to the test tube.…”

Section: Total Phenolic Content Analysismentioning

confidence: 99%

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

Karkar¹,

Şahin²,

Güneş³

2018

GIDA

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Chestnut bee pollen is a high antioxidative natural bee product. In this study the phenolic compounds in chestnut bee pollen were determined by HPLC-DAD system. Ethanol, water and methanol were used for the extraction of antioxidant compounds from chestnut bee pollen. The total phenolic contents and antioxidant capacities of extracts were determined by Folin-Ciocalteu, CHROMAC (Cr(VI) reduction antioxidant capacity) and FRAP (ferric reducing antioxidant power) methods, respectively. When compared the amounts of phenolic compounds and spectroscopic results (total phenolic contents and antioxidant capacities), the most suitable solvent was found ethanol for the extraction of antioxidant compounds from bee pollen. The major phenolic compounds in the chestnut bee pollen were determined as pinocembrin, chrysin, galangin and hyperoside with the contents of 1.246, 0.332, 0.122 and 0.516 mg/g of bee pollen, respectively. And also it was determined that the chestnut bee pollen was inhibited 11% of DNA oxidation in Fenton medium.

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“…The antioxidant capacities of the CBPE were determined with ferric reducing antioxidant power (FRAP) (Benzine & Strain., ), chromium reducing antioxidant capacity (CHROMAC) (Nasir, Şahin, Çakmak, & Çakmak, ), and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) (Aybastıer et al, ) methods. In the FRAP method, 0.25 ml of extract and tripyridyltriazine (2.75 ml) were mixed and left to stand at 37°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Three kinds of solutions were used in the Folin–Ciocalteu method (Aybastıer et al, ): Lowry A, B, and C solutions. The 0.4% NaOH and 2% Na 2 CO 3 were dissolved in distilled water (100 ml) for the Lowry A solution, and the solids of CuSO 4 (0.5 g) and NaKC 4 H 4 O 6 (1 g) were dissolved in distilled water (100 ml) for the Lowry B solution.…”

Section: Methodsmentioning

confidence: 99%

The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases

Şahin

Karkar

2019

J Food Biochem

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Chestnut bee pollen has potential nutritional and medicinal effects and is an important natural bee product. This study focused on the investigation of the antioxidant capacity and DNA damage inhibition ability of chestnut bee pollen (CBP) from Bursa (Turkey). The phenolic compounds (rosmarinic acid, vitexin, hyperoside, pinocembrin, trans‐chalcone, apigenin, protocatechuic, and galangin) and carotenoids in CBPE were determined by HPLC‐DAD (high‐performance liquid chromatography‐diode array detection). Additionally, the protective ability of CBPE against DNA damage by oxidation was investigated. In this study, it was determined that CBPE has a high total phenolic compound content, and the antioxidant capacity of CBPE inhibits DNA oxidation (34% reduction of DNA damage in Fenton reaction media). This study could reveal new information regarding the use of CBPE as a protective agent for DNA in the future. Practical applications Phenolic compounds and carotenoids prevent some diseases because of their important biological activities. One of the potential food sources chestnut bee pollen contains sugar, carbohydrates, amino acids, proteins, lipids, vitamins, hormones, enzymes, and flavonoids. Chestnut bee pollen, which has protective activity against DNA oxidation, could be an excellent potential source of a protective agent against some degenerative diseases through future applications.

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Response Surface Optimized Ultrasonic-Assisted Extraction of Quercetin and Isolation of Phenolic Compounds FromHypericum perforatumL. by Column Chromatography

Cited by 25 publications

References 31 publications

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases

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