Response surface methodology for lovastatin production byAspergillus terreusGD₁₃strain

Abstract

A wild type Aspergillus terreus GD13 strain, chosen after extensive screening, was optimized for lovastatin production using statistical Box-Behnken design of experiments. The interactive effect of four process parameters, i.e. lactose and soybean meal, inoculum size (spore concentration) and age of the spore culture, on the production of lovastatin was evaluated employing response surface methodology (RSM). The model highlighted the positive effect of soybean meal concentration and inoculum level for achieving maximal level of lovastatin (1342 mg/l). The optimal fermentation conditions improved the lovastatin titre by 7.0-folds when compared to the titres obtained under unoptimized conditions.

Screening and genetic engineering of marine-derived Aspergillus terreus for high-efficient production of lovastatin

“…Among them, the mutant LovE-b19, whose lovE gene under the control of P4 promoter, produced the highest level of lovastatin at the titer of 1512 mg/L (Fig. 7A), representing the highest yield recorded, which was significantly higher than that of the highest production level of lovastatin reported (1342 mg/L) [24][25][26][27][28][29][30][31] (Table 2). The transcription of lovE in these mutants was then analyzed.…”

Improving statins production: From non‐genetic strategies to genetic strategies

“…They found it can lead to the maximal lovastatin titer of 1342 mg L −1 when the initial C: N ratio in the culture medium was 37:1, which was seven‐fold compared to the titer obtained under unoptimized conditions. [ 35 ] Additionally, SmF using agricultural waste as a fermentation substrate has gained attention for its environmental benefits and resource recycling. Using A. terreus KPR 12 to ferment the sago processing wastewater, getting 429.98 mg L −1 lovastatin.…”

Strategies for the statins production in microbial cell factory：present and future

“…Oliveira et al (Oliveira, Paulo et al 2021) showed that 60 g/L soluble starch, 15 g/L soybean flour led to producing 100.86 mg/L lovastatin using A. terreus URM 5579. Kaur et al(Kaur, Kaur et al 2010) optimized the culture-medium parameters of A. terreus GD 13 . They found it can lead to the maximal lovastatin titer of 1342 mg/L when the initial C:N ratio in the culture medium was 37:1, which was 7-fold compared to the titer obtained under unoptimized conditions.…”