Response of normal female human osteoblasts (NHOst) to 17β‐estradiol is modulated by implant surface morphology

Lohmann, Christoph H.; Tandy, Elizabeth M.; Sylvia, V. L.; Hell-Vocke, A. K.; Cochran, David L.; Dean, David D.; Boyan, Barbara D.; Schwartz, Zvi

doi:10.1002/jbm.10290

Cited by 73 publications

(47 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because these substrates are produced using the same manufacturing conditions used to produce dental implants, quality control is high and the results we obtain are more readily comparable to clinical observations. Using these substrates, we have found that MG63 osteoblast-like cells, normal human osteoblasts (NHOst cells), and fetal rat calvarial cells exhibit similar behaviors on tissue culture polystyrene (plastic) and smooth PT surfaces with respect to proliferation and differentiation [5][6][7]. In addition, they exhibit a decrease in proliferation and an increase in differentiation when treated with 1α,25(OH) 2 D 3 , that is typical of osteoblasts reported in the literature by many laboratories.…”

Section: Introductionmentioning

confidence: 52%

Beta-1 integrins mediate substrate dependent effects of 1α,25(OH)2D3 on osteoblasts

Schwartz

Bell

Wang

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Surface microroughness increases osteoblast differentiation and enhances responses of osteoblasts to 1,25-dihydroxyvitamin D 3 [1α,25(OH) 2 D 3 ]. β 1 integrin expression is increased in osteoblasts grown on Ti substrates with rough microarchitecture, and it is regulated by 1α,25(OH) 2 D 3 in a surface-dependent manner, suggesting that it has a role in mediating osteoblast response. Here, we silenced β 1 expression in MG63 human osteoblast-like cells using small interfering RNA (siRNA) and examined the responses of the β 1 -silenced osteoblasts to surface microtopography and 1α,25 (OH) 2 D 3 . MG63 cells were also treated with two different monoclonal antibodies to human β 1 to block ligand binding. β 1 -silenced MG63 cells grown on a tissue culture plastic had reduced alkaline phosphatase activity and levels of osteocalcin, transforming growth factor β1, prostaglandin E 2 , and osteoprotegerin in comparison with control cells. Moreover, β 1 -silencing inhibited the effects of surface roughness on these parameters and partially inhibited effects of 1α,25(OH) 2 D 3 . Anti β 1 antibody AIIB2 had no significant effect on cell number and osteocalcin, but decreased alkaline phosphatase; MAB2253Z decreased cell number and alkaline phosphatase and increased osteocalcin in a dose-dependent manner. Effects of 1α,25(OH) 2 D 3 on cell number and alkaline phosphatase were reduced and effects on osteocalcin were increased. These findings indicate that β 1 plays a major and complex role in osteoblastic differentiation modulated by either surface microarchitecture or 1α,25 (OH) 2 D 3 . The results also show that β 1 mediates, in part, the synergistic effects of surface roughness and 1α,25(OH) 2 D 3 .

show abstract

Section: Introductionmentioning

confidence: 52%

Beta-1 integrins mediate substrate dependent effects of 1α,25(OH)2D3 on osteoblasts

Schwartz

Bell

Wang

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

show abstract

“…However, in clinical situations, sex is an important factor that affects musculoskeletal health (Tosi et al 2006). We have shown that female osteoblasts are sensitive to surface microroughness and that 17β-estradiol (E 2 ) plays a role in modulating their response (Lohmann et al 2002). Although male and female cells both show increasing production of osteocalcin, TGF-β1, osteoprotegerin, and prostaglandin E2 on rough SLA versus smooth tissue culture polystyrene and PT surfaces, only female osteoblasts show a roughness-dependent increase in differentiation and local factor production in response to treatment with E 2 and E 2 that is conjugated to bovine serum albumin (Olivares-Navarrete, Hyzy, Chaudhri, et al 2010).…”

Section: Clinical Variablesmentioning

confidence: 99%

Implant Surface Design Regulates Mesenchymal Stem Cell Differentiation and Maturation

et al. 2016

Self Cite

View full text Add to dashboard Cite

Changes in dental implant materials, structural design, and surface properties can all affect biological response. While bulk properties are important for mechanical stability of the implant, surface design ultimately contributes to osseointegration. This article reviews the surface parameters of dental implant materials that contribute to improved cell response and osseointegration. In particular, we focus on how surface design affects mesenchymal cell response and differentiation into the osteoblast lineage. Surface roughness has been largely studied at the microscale, but recent studies have highlighted the importance of hierarchical micron/submicron/nanosurface roughness, as well as surface roughness in combination with surface wettability. Integrins are transmembrane receptors that recognize changes in the surface and mediate downstream signaling pathways. Specifically, the noncanonical Wnt5a pathway has been implicated in osteoblastic differentiation of cells on titanium implant surfaces. However, much remains to be elucidated. Only recently have studies been conducted on the differences in biological response to implants based on sex, age, and clinical factors; these all point toward differences that advocate for patient-specific implant design. Finally, challenges in implant surface characterization must be addressed to optimize and compare data across studies. An understanding of both the science and the biology of the materials is crucial for developing novel dental implant materials and surface modifications for improved osseointegration.

show abstract

“…Cells cultured on microrough Ti surfaces also exhibit decreased proliferation and increased differentiation [8,10]. Moreover, rough Ti surfaces increase osteoblast response to hormones and growth factors, including 1,25-dihydroxyvitamin D 3 [1α,25(OH) 2 D 3 ], 17β-estradiol and bone morphogenetic protein [11][12][13]. On rough Ti surfaces, osteoblasts also generate an osteogenic microenvironment to regulate bone remodeling, represented by releasing local factors to promote osteoblast differentiation and inhibit osteoclast activation [1].…”

Section: Introductionmentioning

confidence: 99%

Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography

et al. 2007

View full text Add to dashboard Cite

Objective-Surface roughness and surface free energy are two important factors that regulate cell responses to biomaterials. Previous studies established that titanium substrates with micron-scale and submicron scale topographies promote osteoblast differentiation and osteogenic local factor production and that there is a synergistic response to microrough Ti surfaces that have retained their high surface energy via processing that limits hydrocarbon contamination. This study tested the hypothesis that the synergistic response of osteoblasts to these modified surfaces depends on both surface microstructure and surface energy.Methods-Ti disks were manufactured to present three different surface structures: smooth pretreatment surfaces (PT) with R a of 0.2 µm; acid-etched surfaces (A) with a submicron roughness R a of 0.83 µm; and sandblasted/acid-etched surfaces (SLA) with R a of 3-4 µm. Modified acid-etched (modA) and modified sandblasted/acid-etched (modSLA) titanium substrates, which have low contamination and present a hydroxylated/hydrated surface layer to retain high surface energy, were compared with regular low surface energy A and SLA surfaces. Human osteoblast-like MG63 cells were cultured on these substrates and their responses, including cell shape, growth, differentiation (alkaline phosphatase, osteocalcin), and local factor production (TGF-β1, PGE 2 , osteoprotegerin [OPG]) were analyzed (N=6 per variable). Data were normalized to cell number.Results-There were no significant differences between smooth PT and A surfaces except for a small increase in OPG. Compared to A surfaces, MG63 cells produced 30% more osteocalcin on modA, and 70% more on SLA. However, growth on modSLA increased osteocalcin by more than 250%, which exceeded the sum of independent effects of surface energy and topography. Similar effects were noted when levels of latent TGF-β1, PGE 2 and OPG were measured in the conditioned media.Conclusions-The results demonstrate a synergistic effect between high surface energy and topography of Ti substrates and show that both micron scale and submicron scale structural features are necessary.

show abstract

Response of normal female human osteoblasts (NHOst) to 17β‐estradiol is modulated by implant surface morphology

Cited by 73 publications

References 43 publications

Beta-1 integrins mediate substrate dependent effects of 1α,25(OH)2D3 on osteoblasts

Beta-1 integrins mediate substrate dependent effects of 1α,25(OH)2D3 on osteoblasts

Implant Surface Design Regulates Mesenchymal Stem Cell Differentiation and Maturation

Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography

Contact Info

Product

Resources

About