Response of lymphatic tissues to natural feed additives, curcumin (Curcuma longa) and black cumin seeds (Nigella sativa), in broilers against Pasteurella multocida

Abstract

The antibiotic residues and pathogenic resistance against the drug are very common in poultry because of antibiotics used in their feed. It is necessary to use natural feed additives as effective alternatives instead of a synthetic antibiotic. This study aimed to investigate the immune response of Nigella sativa and Curcuma longa in broilers under biological stress against Pasteurella multocida . The total 100, one-day-old chicks were divided into 5 groups. Groups 1 and 2 served as control negative and control positive. Both control groups were receiving simple diet without any natural feed additives, but the infection was given in group 2 at day 28 with the dose of 5.14 × 10 7 CFU by IV. Groups 3A and 3B were offered 2% seed powder of Nigella sativ a, groups 4A and 4B were offered C. longa 1% in powdered form, and group 5A and 5B were offered both C. longa 1% and N. sativa 2% in the feed from day 1 and groups 3B, 4B, and 5B were challenged with P. multocida . The haemagglutination inhibition titter against Newcastle Disease virus (NDV), feed conversion ratio, mortality, gross, and histopathology were studied. The results of this study revealed that hemagglutination inhibition titers against NDV were highly significant ( P < 0.05) in treated groups, highest titers (3A, 6.8; 3B, 6.4; and 5A, 7.2) were obtained from treated Groups. The feed conversion ratio of N. sativa + C. longa treated groups (5A, 1.57, and 3A, 1.76) were higher than that of other nontreated groups. The gross and histopathological changes were much severe in control positive, but fewer changes were seen in treated groups. Therefore, we recommend that natural feed additives, black cumin (N. sativa) and turmeric ( C. longa ), act as an immune enhancer in broilers against P. multocida.

Physical quality of KUB chicken carcass supplemented with turmeric

“…Equivalent to the carcass yield, turmeric treatments did not affect liver percentage in KUB chickens (p>0.05). A similar study also revealed that herbal supplementation did not alter liver percentage in kampung chickens [11]. This condition indicates that turmeric supplementation is safe and has no adverse effect since there is no alteration in the liver tissue of KUB chickens.…”

“…The parameter observed included carcass and its cut parts, liver, and abdominal fat percentages. Each parameter was calculated as the percentage of live body weight [5,11].…”

Immune stimulatory effect of Nigella sativa in healthy animal models: A systematic review and meta-analysis

“…Seventeen studies were eligible and relevant to the intervention (i.e., NSS) and the prespecified outcomes in healthy animals [ [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] ]. All 17 studies were assessed for internal validity (risk of bias (RoB) assessment).…”

“…Four studies reported that NSS significantly increased serum titers of anti-NDV antibodies [ 31 , 33 , 34 , 39 ]. Conversely, four studies reported that NSS failed to induce significant changes in serum anti-NDV titers [ 25 , 29 , 30 , 38 ].…”

“…For serum anti-SRBC titers, six reports were included [ 26 , 31 , 32 , 37 , 40 ], among them two reports by Al-Khalifa, Al-Nasser [ 26 ]. The overall pooled effect size of NSS on serum anti-SRBC titers was significantly different in favor of the NSS group [MD = 1.15, 95% CI: 0.74 to 1.56, p = 0.00001, with zero heterogeneity (I 2 = 0%, p = 0.43] ( Fig.…”