Response of In Vitro Strawberry to Silver Nitrate (AgNO3)

Qin, Yonghua; Zhang, Shanglong; Zhang, Lingxiao; Zhu, Daoyu; Asghar, Syed

doi:10.21273/hortsci.40.3.747

Cited by 30 publications

(18 citation statements)

References 20 publications

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“…-treated Q. rubra cultures in this study-shoot tip necrosis and leaf abscission-have been observed in shoot cultures of Annona squamosa (Armstrong et al 1997), Holostemma ada-Kodien (Martin 2002) and habanero pepper (Santana-Buzzy et al 2005). AgNO 3 and silver thiosulphate have previously been reported to have beneficial effects on shoot growth and organogenesis in a variety of culture systems (Faria and Segura 1997;Reis et al 2003;Burgos and Alburquerque 2003;Alaska-Kennedy et al 2005;Qin et al 2005), but as far as we know there have hitherto been no studies of the effects of ethylene inhibitors on the growth and development of oak shoots in vitro. It may be noted that in the concentration range used in this study, AgNO 3 appears not to have any of its known undesirable side effects (Kumar et al 1998).…”

Section: Discussionmentioning

confidence: 53%

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Viéitez

Corredoira

Ballester

et al. 2009

Plant Cell Tiss Organ Cult

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North American oak species, with their characteristic strong episodic seasonal shoot growth, are highly problematic for clonal micropropagation, resulting in the inability to achieve a stabilized shoot multiplication stage. The potential for initiating and proliferating shoot cultures derived from Quercus alba, Q. bicolor and Q. rubra explants was investigated, and a micropropagation method for these species was developed. Branch segments from 6 to 7-year-old trees were forced-flushed and the forced shoots were used as source of explants for culture initiation. A consistent shoot multiplication stage was achieved, in 13 of the 15 genotypes established in vitro, although marked differences occurred in explants from different genotypes/species. The control of efficient shoot multiplication involved the culture of decapitated shoots in a stressful horizontal position on cytokinin-containing medium with a sequence of transfers within a 6-week subculture cycle, which was beneficial to overcoming the episodic character of shoot growth. During each subculture cycle, the horizontally placed explants were cultured on media containing 0.2 mg l -1 benzyladenine (BA) for 2 weeks with two successive transfers (2 weeks each) to fresh medium with 0.1 mg l -1 BA, giving a 6-week subculture cycle. The general appearance and vigor of Q. alba and Q. bicolor shoot cultures were improved by the inclusion of both 0.1 mg l -1 BA and 0.5 mg l -1 zeatin in the medium used for the second transfer within the 6-week subculture cycle. Addition of AgNO 3 (3 mg l -1 ) to the shoot proliferation medium of Q. rubra had a significant positive effect on shoot development pattern by reducing deleterious symptoms, including shoot tip necrosis and early senescence of leaves. The three species showed acceptable in vitro rooting rates by culturing microcuttings in medium containing 25 mg l -1 indolebutyric acid for 48 h with subsequent transfer to auxin-free medium supplemented with 0.4% activated charcoal. Although an initial 5-day dark period generally improved the rooting response, it was detrimental to the quality of regenerated plantlets. However, activated charcoal stimulated not only the rooting frequencies, but it also enhanced plant quality, as evidenced by root, shoot and leaf growth.

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Section: Discussionmentioning

confidence: 53%

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Viéitez

Corredoira

Ballester

et al. 2009

Plant Cell Tiss Organ Cult

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“…Similar to our findings, Huang and Liu (2002) observed higher levels of reducing sugars and starch in regenerating callus than in nonregenerating ones in Oryza sativa. Qin et al (2005) also found increases in soluble protein content and higher proline accumulation during shoot regeneration in strawberry callus cultures. However, phenols showed reverse trends and were higher in nonregenerating calluses than in regenerating calluses.…”

Section: Resultsmentioning

confidence: 71%

“…Similarly, comparably higher activities of antioxidant enzymes like CAT, POX, PPOX, and H 2 O 2 content were observed generally in regenerating calluses than in nonregenerating calluses (Table 6). Chen and Ziv (2001) in Narcissus and Qin et al (2005) in strawberry correlated higher CAT activity during initial stages with shoot regeneration in calluses of regenerating nature. Libik et al (2005) found a higher concentration of H 2 O 2 in morphogenic callus and consequently supported the hypothesis that H 2 O 2 may be produced in excess due the disruption of oxidative balance which in turn may promote the expression of genes responsible for the initiation of morphogenesis.…”

Section: Resultsmentioning

confidence: 86%

Indirect organogenesis and plant regeneration in Helicteres isora L., an important medicinal plant

Shriram

Kumar

Shitole

2008

In Vitro Cell.Dev.Biol.-Plant

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Helicteres isora is a medicinal plant effective against asthma, diabetes, hypolipidemia, HIV, polio besides a good source of diosgenin. Seed dormancy and low natural fruit production rate make this plant a perfect candidate for developing an in vitro regeneration method. However, to date, no such work has been procured in this plant. An efficient method for plant regeneration via shoot organogenesis from callus cultures has been developed using nodal explants in H. isora. Murashige and Skoog (MS) media counting 2,4-Dichlorophenoxyacetic acid (2,4-D, 2.26 to 13.57 μM), Indole-3-acetic acid (IAA, 2.85 to 17.13 μM), Indole-3-butyric acid (IBA, 2.46 to 14.70 μM), 6-Benzylaminopurine (BA, 2.22 to 13.32 μM) and Kinetin (Kin, 2.32 to 13.92 μM) either singly or in the following combinations (IAA + BA; IAA + Kin, and BA + Kin) produced granular callus except BA + Kin which resulted in compact, hard, greenish-white (CHGW) callus. The optimum CHGW callus (2.62 g fresh weight/ explant) was produced on MS media with 13.32 μM BA + 2.32 μM Kin with over 93% callus induction frequency. Optimum shoot organogenesis (67% frequency) was achieved in CHGW callus with lower level of BA (2.22 μM) and Kin (2.32 μM) and produced 3.2 shoots/0.5 g callus within 35 d of culture. Microshoots were rooted successfully (62% frequency) after 35 d of culture on 1/2MS containing 4.90 μM IBA and hardened off. Antioxidant enzymes such as catalase, peroxidase, polyphenol oxidase, and biochemical parameters viz. hydrogen peroxide, reducing and nonreducing sugars, starch, proteins, phenols, and proline contents were studied in regenerating and nonregenerating CHGW calluses to establish a correlation between these parameters and shoot morphogenesis. All the enzyme activities and biochemical parameters were found more in regenerating callus than in nonregenerating except phenols. induction frequency. Optimal shoot organogenesis (67% frequency) was achieved in CHGW callus with lower levels of BA (2.22 μM) and Kin (2.32 μM) and produced 3.2 shoots/0.5 g callus within 35 d of culture. Microshoots were rooted and hardened successfully (62% frequency) after 35 d of culture on 1/2-MS containing 4.90 μM IBA. Antioxidant enzymes (catalase, peroxidase, polyphenol oxidase) and biochemical parameters viz. hydrogen peroxide, reducing and nonreducing sugars, starch, proteins, phenols, and proline contents were studied in regenerating and nonregenerating CHGW calluses to establish a correlation between these parameters and shoot morphogenesis. With the exception of phenols, all the enzyme activities and biochemical parameters were found more frequently in regenerating than in nonregenerating callus.

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“…Individual shoots (2.0 cm in length) from propagated shoots were teased out and transferred to rooting medium (Qin et al 2005b; half-strength MS basal medium ? 3% sucrose ?…”

Section: Media and Culture Conditionsmentioning

confidence: 99%

Response of in vitro strawberry to antibiotics

Qin

Silva

et al. 2011

Plant Growth Regul

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By identifying antibiotics that had the least phytotoxic effects on explants during genetic transformation, we evaluated the effect of various antibiotics on callus induction and morphogenesis from leaf explants and in vitro growth of Fragaria 9 ananassa Duch. cv. Toyonaka. Results showed that kanamycin (Kan) significantly inhibited callus induction, bud differentiation and root morphogenesis while carbenicillin (Carb), cefotaxime (Cef) and an equal concentration of Cef and Carb up to 500 mg L -1 had no significant effects on callus induction and shoot growth. Kan, even at 2.5 mg L -1 , significantly inhibited callus induction, shoot regeneration and root formation, while no shoots regenerated at concentrations above 15 mg L -1 . Rooting was completely inhibited in the presence of 50 mg L -1 Kan. Cef had negative effects on shoot regeneration from leaf explants and in vitro growth of strawberry. Compared to Cef, Carb at B300 mg L -1 significantly promoted shoot and root organogenesis. However, an equal concentration of Carb plus Cef could alleviate the negative effect of Cef on strawberry. Results from relative electrolyte leakage, root and antioxidant activities, O 2 Á-production rate, H 2 O 2 , proline and MDA contents showed that Kan, Cef and Carb caused electrolyte

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Response of In Vitro Strawberry to Silver Nitrate (AgNO3)

Cited by 30 publications

References 20 publications

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Indirect organogenesis and plant regeneration in Helicteres isora L., an important medicinal plant

Response of in vitro strawberry to antibiotics

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