Response of in vitro strawberry to antibiotics

Qin, Yonghua; Silva, Jaime A. Teixeira da; Bi, Jiapeng; Zhang, S. L.; Hu, Gui

doi:10.1007/s10725-011-9587-9

Cited by 42 publications

(34 citation statements)

References 45 publications

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“…In the strawberry, like regeneration, the antibiotic sensitivity response varies depending on the cultivar and explant type; however, their effects on morphogenesis, shoot growth and physiological changes have not been well reported (QIN et al, 2011) However, reducing the levels of kanamycin to 0, 2, 4, 6, 8 and 10 mg L -1 (this study), we observed an inhibition curve where increasing concentrations of kanamycin induced decreases in bud and shoot formation, in accordance with Qin et al (2011), culminating in complete callus, bud and shoot inhibition at 10 mg L -1 for both varieties ( Figure 3 and Figure 4). In addition, we also observed increased necrosis according to kanamycin dose, possibly due to electrolyte leakage and triggering of active enzymatic processes (PANTAZIS et al, 2013).…”

Section: Experiments IImentioning

confidence: 99%

“…However, biotechnology and genetic engineering techniques are being extensively used in strawberry breeding and to increase the availability of germoplasm (QIN et al, 2008). Among these techniques, genetic transformation by Agrobacterium tumefaciens is a standard and efficient method applied to strawberry breeding (DEBNATH; TEIXEIRA DA SILVA, 2007); nonetheless, this depends not only on the use of an appropriate transformation protocol, but also on the prior establishment of an efficient system to recover and identify transformed plants (QIN et al, 2011).…”

Section: Introdutionmentioning

confidence: 99%

“…Marker genes that induce resistance to substances such as antibiotics assist in transformed plant selection by inhibiting untransformed shoot growth (ABDAL-AZIZ et al, 2006;KARAMI et al, 2009;QIN et al, 2011). Many transformation methods use the nptII gene, which confers resistance to the aminoglycoside group of antibiotics (neomycin, kanamycin, and gentamicin), to transform cells (MARCELLINO et al, 2007;QIN et al, 2011;PANTAZIS et al, 2013). However, the selective agents can have a negative effect on callus induction and regeneration capacity and vary according to the concentration, species, cultivar and explant type (KARAMI et al, 2009).…”

Section: Introdutionmentioning

confidence: 99%

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Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

Reis

Ayub

2015

Sem. Ci. Agr.

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The success of genetic transformation of the strawberry (Fragaria x ananassa) is strongly influenced by the cultivar and depends on efficient regeneration and plant-transformed selection. In plant regeneration, efficient organogenesis depends on the concentrations of plant growth regulators that are present in the culture medium, and the response varies according to the explant type and cultivar. Similarly, in the selection of transformed plants, the sensitivity to selective agents induced by marker genes depends on the concentration of the selective substance in the regeneration medium and must be adjusted according to the chosen cultivar. The aim of this work was to test different combinations and concentrations of TDZ (Thidiazuron) ResumoO sucesso da transformação genética do morangueiro (Fragaria x ananassa) é grandemente influenciado pela cultivar utilizada e dependente da eficiente regeneração e seleção do material transformado. Na regeneração das plantas, a organogênese depende da concentração de reguladores de crescimento presente no meio de cultivo e varia de acordo com a fonte de explante e a cultivar utilizada. Da mesma forma, na seleção das plantas transformadas, a sensibilidade à agentes seletivos induzida por genes marcadores, depende da concentração da substância seletiva no meio de regeneração e deve ser ajustada de acordo com a cultivar utilizada. O objetivo deste trabalho foi testar diferentes combinações e concentrações de TDZ (Thidiazuron) e AIB (Ácido Indolbutírico) adicionados ao meio básico MS

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Section: Experiments IImentioning

confidence: 99%

Section: Introdutionmentioning

confidence: 99%

Section: Introdutionmentioning

confidence: 99%

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Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

Reis

Ayub

2015

Sem. Ci. Agr.

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“…This group of antibiotics act by interfering with penicillin-binding proteins and by inhibiting the biosynthesis of the peptidoglycan network of the cell wall that provokes cell wall lysis and consequently bacterial death [12]. However, it has been shown that the application of β-lactam antibiotics frequently affects plant growth and development, leading to either the inhibition [13,14] or the promotion [15][16][17][18] of regeneration ability. They may differently affect callus induction [14], shoot formation [19], somatic embryogenesis [20] or rooting [13].…”

Section: Introductionmentioning

confidence: 99%

“…However, it has been shown that the application of β-lactam antibiotics frequently affects plant growth and development, leading to either the inhibition [13,14] or the promotion [15][16][17][18] of regeneration ability. They may differently affect callus induction [14], shoot formation [19], somatic embryogenesis [20] or rooting [13]. It is known that plant sensitivity to antibiotics is species-, genotype-or even tissue-specific [21].…”

Section: Introductionmentioning

confidence: 99%

The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

Stanišić

Ninković

Savić

et al. 2018

ARCH BIOL SCI BELGRA

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Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L -1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L -1 ). Cefotaxime at a concentration of 300 mg L -1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L -1 ) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L -1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L -1 throughout organogenesis and 10 mg L -1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious. Abbreviations: BAP -N 6 -benzilaminopurin; BM − basal medium; IBA -indole-3-butyric acid; MIC -minimal inhibitory concentration; MS -Murashige and Skoog mineral solution; NAA -α-naphthaleneacetic acid; HPThygromycin B phosphotransferase; SFC -the index of shoot-forming capacity; SMM -shoot multiplication medium; SRM -shoot regeneration medium; TDZ -thidiazuron How to cite this article: Stanišić M, Ninković S, Savić J, Ćosić T, Mitić N. The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. Arch Biol Sci. 2018;70(1):179-90.

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Strawberry (Fragaria × ananassa)

Cappelletti

Sabbadini

Mezzetti

2014

Methods in Molecular Biology

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Genetic transformation in strawberry (Fragaria spp.) can be achieved by using the Agrobacterium-mediated procedure on leaves from in vitro proliferated shoots. Regardless of the sufficient regeneration levels achieved from leaf explants of some commercial strawberry genotypes, the regeneration of transformed strawberry plants remains difficult and seems to be strongly genotype dependent. In fact, the main factors that play an important role in the success of strawberry genetic transformation are the availability of an efficient regeneration protocol and of an appropriate selection procedure of the putative transgenic shoots. The strawberry genetic transformation protocol herein described relates to three genotypes resulted from our experience with the highest regeneration and transformation efficiency. The study includes two octoploid Fragaria × ananassa cultivars, Sveva and Calypso, and a diploid F. vesca cultivar (Alpina W.O.). All the different steps related to the leaf tissue Agrobacterium infection, coculture, and selection of regenerating adventitious shoots, as well as the following identification of selected lines able to proliferate and root on the selective agent (kanamycin), will be described.

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Response of in vitro strawberry to antibiotics

Cited by 42 publications

References 45 publications

Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

Strawberry (Fragaria × ananassa)

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