Response of chloramphenicol-reducing biocathode resistome to continuous electrical stimulation

Liang, Bin; Ma, Jincai; Cai, Weiwei; Li, Zhiling; Liu, Wenzong; Qi, Mengyuan; Zhao, Yongjian; Ma, Xiaodan; Deng, Ye; Wang, Aijie; Zhou, Jizhong

doi:10.1016/j.watres.2018.10.073

Cited by 96 publications

(24 citation statements)

References 50 publications

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“…Bioelectrochemical systems (BES) employing electrode-respiring microbes in cathodes could effectively reductively remove CAP [12]. Furthermore, Liang et al [13] found that the antibacterial activity of CAP was quickly eliminated during the CAP bioelectroreduction process in BES. However, reduction of CAP on the biocathode significantly relied on the microbes with extracellular electron transfer ability [14].…”

Section: Introductionmentioning

confidence: 99%

Reductive degradation of chloramphenicol by Geobacter metallireducens

Xiao

Zheng

Zhang

et al. 2019

Sci. China Technol. Sci.

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Geobacter metallireducens is known to be capable of removing nitroaromatic compounds via an oxidation mode. However, little attention has been paid to investigate the reductive removal of chlorinated nitroaromatic compounds by G. metallireducens. In this study, G. metallireducens was used to reduce chloramphenicol (CAP), a typical chlorinated nitroaromatic antibiotic. Cyclic voltammograms and chronoamperometry highlighted a higher peak current for CAP reduction by G. metallireducens compared to the control without bacteria. G. metallireducens efficiently reduced CAP (20 mg/L) with acetate as the sole electron donor, and the removal efficiency reached (97.6±4.9)% within 6 d. Aromatic amine (AMCl2), AMCl (dechlorinated AMCl2) and AM (dechlorinated AMCl) were identified as reduction products by liquid chromatography-mass spectrometry. However, the removal efficiency declined to (25.0±3.6)% when the CAP dosage increased to 80 mg/L. Transcriptomic analysis indicated the significant upregulation of genes related to electron transfer, such as pilus assembly protein gene (2.8 folds), NADH-quinone oxidoreductase subunit K2 gene (4.5 folds) and many c-type cytochrome genes such as cytochrome c biogenesis protein ResB (Gmet 2901, 4.6 folds), cytochrome c (Gmet 0335, 4.4 folds) and cytochrome c7 (Gmet 2902, 3.4 folds). Furthermore, a gene related to chlorinated contaminant removal (Gmet 1046, 5.4 folds) was also upregulated, possibly resulting in enhanced CAP reduction. This work deepened our knowledge of the bioremediation ability of G. metallireducens with respect to environmental contaminants and provided a potential strategy to treat antibiotics with electrochemically active bacteria.

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Section: Introductionmentioning

confidence: 99%

Reductive degradation of chloramphenicol by Geobacter metallireducens

Xiao

Zheng

Zhang

et al. 2019

Sci. China Technol. Sci.

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“…This makes it a specific technology that potentially can give quantitative results. The use of GeoChip has grown to include its use in ecosystem ARG detection due to its power in providing sensitive, specific, and quantitative information with good repeatability in water [11,113,114] and extreme environments [115]. However, there are currently no research publications that have used this technology to analyze ARG pollution caused by animal husbandry.…”

Section: Metagenomic Analysismentioning

confidence: 99%

“…Animal-derived pathogens can also easily assimilate ARGs that can then be transferred to humans by contact or consumption of raw fruits and vegetables containing these pathogens; this poses a threat to public health [8]. Even the most advanced wastewater treatment procedures cannot remove all ARB before discharge [9][10][11][12]. The associated risks and mechanisms of ARG transmission, transmission pathways, and technical methods have been studied with fruitful results being achieved, but there are still many unknown factors.…”

Section: Introductionmentioning

confidence: 99%

Research and Technological Advances Regarding the Study of the Spread of Antimicrobial Resistance Genes and Antimicrobial-Resistant Bacteria Related to Animal Husbandry

Liu

Zhang

et al. 2019

IJERPH

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The extensive use of antimicrobials in animal farms poses serious safety hazards to both the environment and public health, and this trend is likely to continue. Antimicrobial resistance genes (ARGs) are a class of emerging pollutants that are difficult to remove once introduced. Understanding the environmental transfer of antimicrobial-resistant bacteria (ARB) and ARGs is pivotal for creating control measures. In this review, we summarize the research progress on the spread and detection of ARB and ARG pollution related to animal husbandry. Molecular methods such as high-throughput sequencing have greatly enriched the information about ARB communities. However, it remains challenging to delineate mechanisms regarding ARG induction, transmission, and tempo-spatial changes in the whole process, from animal husbandry to multiple ecosystems. As a result, future research should be more focused on the mechanisms of ARG induction, transmission, and control. We also expect that future research will rely more heavily on metagenomic -analysis, metatranscriptomic sequencing, and multi-omics technologies

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“…Sediment microbial fuel cells (SMFCs) is a subgroup of microbial fuel cells (MFCs) that can convert the chemical energy stored in sediments to electricity via microbial extracellular electron transfer. Several pilot‐scale SMFCs have shown many advantages for remote power supply and sediment bioremediation . However, due to the slow chemical diffusion in sediments, SMFC power outputs are lower than MFCs with wastewater (WMFCs) and cannot meet the needs of practical electronic devices .…”

Section: Introductionmentioning

confidence: 99%

“…Several pilot-scale SMFCs have shown many advantages for remote power supply and sediment bioremediation. [2][3][4] However, due to the slow chemical diffusion in sediments, SMFC power outputs are lower than MFCs with wastewater (WMFCs) and cannot meet the needs of practical electronic devices. [5][6][7] Power management systems (PMS) are essential in boosting SMFC power to a utilizable level for electronic devices.…”

Section: Introductionmentioning

confidence: 99%

Effects of unit distance and number on sediment microbial fuel cell stacks for practical power supply

Yang

Yan²,

Lin

et al. 2019

Int J Energy Res

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Summary Sediment microbial fuel cells (SMFCs) can covert the biomass and organic matters in sediments into electricity. SMFC stack is an essential way for the application of SMFCs. The unit distance and number will be crucial for SMFC stacks applied in practical environments. This study showed that the power density of individual SMFC increased with the unit distance when compared with SMFCs with a small distance. For hydraulically connected serial stacks, increasing unit distance from 2 to 28 cm decreased the potential loss from 50.4% to 11.3%, but the power output did not increase with either unit distance or number of units due to higher internal resistance and electrode reversal. For hydraulically connected parallel stacks, increasing unit number from one to three multiplied the power output but no further power increase was observed with four and five units, indicating an optimal unit number (OUN) in parallel stacks due to ion conduction. Similar performance was shown when using SMFC stacks to power a light‐emitting diode and an environmental sensor. The results provide important information for improving the power and cost‐effectiveness of SMFC stacks.

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Response of chloramphenicol-reducing biocathode resistome to continuous electrical stimulation

Cited by 96 publications

References 50 publications

Reductive degradation of chloramphenicol by Geobacter metallireducens

Reductive degradation of chloramphenicol by Geobacter metallireducens

Research and Technological Advances Regarding the Study of the Spread of Antimicrobial Resistance Genes and Antimicrobial-Resistant Bacteria Related to Animal Husbandry

Effects of unit distance and number on sediment microbial fuel cell stacks for practical power supply

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