Methylation of arsenic in soil influences its environmental behavior and accumulation by plants, but little is known about the factors affecting As methylation. As speciation was determined in the pore waters of six soils from diverse geographical locations over 54 days of incubation under flooded conditions. The concentration of methylated As (monomethylarsonic acid, MMA, and dimethylarsinic acid, DMA) varied from 0 to 85 μg L −1 (0 − 69% of the total As in pore water). Two Bangladeshi paddy soils contaminated by irrigation of As-laden groundwater produced large concentrations of inorganic As but relatively little methylated As. Two contaminated paddy soils from China produced a transient peak of DMA during the early phase of incubation. Methylated As represented considerable proportions of the total soluble As in the two uncontaminated soils from the UK and U.S. The copy number of the microbial arsenite methyltransferase gene (arsM) correlated positively with soil pH. However, pore-water methylated As correlated negatively with pH or arsM copy number, and positively with dissolved organic C. GeoChip assay revealed considerable arsM diversity among the six soils, with 27−35 out of 66 sequences in the microarray being detected. As speciation in rice plants grown in the soils generally mirrored that in the pore water. The results suggest that methylated As species in plants originated from the soil and As methylation in soil was influenced strongly by the soil conditions. ■ INTRODUCTIONArsenic (As) is a ubiquitous contaminant in the environment originating from both natural and anthropogenic sources. Because its biogeochemical behavior and toxicity vary greatly among different chemical species, it is important to understand how the speciation of As changes in the environment and what drives such changes. A particularly important case is the paddy rice system because it is now recognized that rice is a major source of As in the human diet. 1 The anaerobic conditions in paddy soils are conducive to the mobilization of arsenite, 2,3 which is taken up inadvertently by rice roots through the strong uptake pathway for silicic acid. 4 Rice grain contains both inorganic As (arsenate and arsenite) and organic As (mostly dimethylarsinic acid, DMA; occasionally also trace amounts of monomethylarsonic acid, MMA, and tetramethylarsonium). 5−9 As speciation in rice varies widely among different riceproducing regions. Market-basket surveys show that Asian rice generally is dominated by inorganic As with DMA typically accounting for about 20% of the total As. [5][6][7]10 In contrast, rice produced in the U.S. and Europe is more variable in As speciation with many samples containing more organic than inorganic As. 5,7,8,10 It is possible that that this geographical pattern reflects the relative bioavailability of inorganic versus organic As in different paddy environments. 10 Compared with inorganic As, methylated As species are more easily accumulated in rice grain. 10 Although pentavalent methylated As species are ...
Microbial communities in terrestrial fresh water are diverse and dynamic in composition due to different environmental factors. The goal of this study was to undertake a comprehensive analysis of bacterial composition along different rivers and creeks and correlate these to land-use practices and pollutant sources. Here we used 454 pyrosequencing to determine the total bacterial community composition, and bacterial communities that are potentially of fecal origin, and of relevance to water quality assessment. The results were analyzed using UniFrac coupled with principal coordinate analysis (PCoA) to compare diversity, abundance, and community composition. Detrended correspondence analysis (DCA) and canonical correspondence analysis (CCA) were used to correlate bacterial composition in streams and creeks to different environmental parameters impacting bacterial communities in the sediment and surface water within the watershed. Bacteria were dominated by the phyla Proteobacteria, Bacteroidetes, Acidobacteria, and Actinobacteria, with Bacteroidetes significantly (P<0.001) higher in all water samples than sediment, where as Acidobacteria and Actinobacteria where significantly higher (P<0.05) in all the sediment samples than surface water. Overall results, using the β diversity measures, coupled with PCoA and DCA showed that bacterial composition in sediment and surface water was significantly different (P<0.001). Also, there were differences in bacterial community composition between agricultural runoff and urban runoff based on parsimony tests using 454 pyrosequencing data. Fecal indicator bacteria in surface water along different creeks and channels were significantly correlated with pH (P<0.01), NO2 (P<0.03), and NH4N (P<0.005); and in the sediment with NO3 (P<0.015). Our results suggest that microbial community compositions were influenced by several environmental factors, and pH, NO2, and NH4 were the major environmental factors driving FIB in surface water based on CCA analysis, while NO3 was the only factor in sediment.
Antibacterial activity elimination a b s t r a c tAntibiotics in wastewaters must be degraded to eliminate their antibacterial activity before discharging into the environment. A cathode can provide continuous electrons for the degradation of refractory pollutants, however the cathodic degradation feasibility, efficiency and pathway for different kinds of antibiotics is poorly understood. Here, we investigated the degradation of four antibiotics, namely nitrofurazone (NFZ), metronidazole (MNZ), chloramphenicol (CAP), and florfenicol (FLO) by a poised cathode in a dual chamber electrochemical reactor. The cyclic voltammetry preliminarily proved the feasibility of the cathodic degradation of these antibiotics. The cathodic reducibility of these antibiotics followed the order of NFZ > MNZ > CAP > FLO. A decreased phosphate buffered solution (PBS) concentration as low as 2 mM or utilization of NaCl buffer solution as catholyte had significant influence on antibiotics degradation rate and efficiency for CAP and FLO but not for NFZ and MNZ. PBS could be replaced by Na 2 CO 3 eNaHCO 3 buffer solution as catholyte for the degradation of these antibiotics. Reductive dechlorination of CAP proceeded only after the reduction of the nitro group to aromatic amine. The composition of the degradation products depended on the cathode potential except for MNZ. The cathodic degradation process could eliminate the antibacterial activity of these antibiotics. The current study suggests that the electrochemical reduction could serve as a potential pretreatment or advanced treatment unit for the treatment of antibiotics containing wastewaters.© 2015 Elsevier Ltd. All rights reserved. IntroductionPharmaceuticals and personal care products (PPCPs), as emerging contaminants, have attracted growing attention worldwide in recent years, which would pose potential threats to aquatic life and even human health (Liu and Wong, 2013 w a t e r r e s e a r c h 7 2 ( 2 0 1 5 ) 2 8 1 e2 9 2http://dx
The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global anaerobic regulatory gene fnr (fumarate nitrate reduction regulator) from E. cloacae in the non-Se-reducing strain Escherichia coli S17-1 activated the ability to reduce Se(VI) and precipitate insoluble Se(0) particles. Se(VI) reduction by E. coli S17-1 containing the fnr gene occurred at rates similar to those for E. cloacae, with first-order reaction constants of k ؍ 2.07 ؋ 10 ؊2 h ؊1 and k ؍ 3.36 ؋ 10 ؊2 h ؊1 , respectively, and produced elemental selenium particles with identical morphologies and short-range atomic orders. Mutation of the fnr gene in E. cloacae SLD1a-1 resulted in derivative strains that were deficient in selenate reductase activity and unable to precipitate elemental selenium. Complementation by the wild-type fnr sequence restored the ability of mutant strains to reduce Se(VI). Our findings suggest that Se(VI) reduction and the precipitation of Se(0) by facultative anaerobes are regulated by oxygen-sensing transcription factors and occur under suboxic conditions.The chemical speciation of selenium controls its mobility in natural waters and biological effect on animal life (5, 27, 44). While selenium is an essential micronutrient (27), the higher valence states of Se(VI) and Se(IV) are toxic at elevated concentrations and can cause severe poisoning of fish and waterfowl in contaminated environments (11,22,32,40). The reduction of soluble selenate [Se(VI), SeO 4 2Ϫ ] and selenite [Se(IV), SeO 3 2Ϫ ] to the less toxic Se(0) converts selenium into an insoluble mineral form. In soils and sediments, this transformation is largely mediated by microorganisms (30,31,43,47), although abiotic reduction of selenium oxyanions can also occur in the presence of the Fe(II)-Fe(III) hydroxide mineral green rust (28). Anaerobic Se-respiring bacteria can use Se(VI) and Se(IV) as terminal electron acceptors and precipitate elemental selenium granules (26,29,30). Aerobic and phototrophic Se-resistant bacteria can also catalyze the reduction of selenium oxyanions to form insoluble Se(0) particles (1,15,35,38). However, despite the ubiquity of Se-reducing bacteria in diverse terrestrial and aquatic environments (P. Narasingarao and M. M. Haggblom, unpublished data), the mechanisms of Se(0) biomineralization are poorly understood.Microbial reduction of selenium oxyanions generates red elemental selenium with either crystalline or amorphous structures (24,25). Recently, Oremland et al. (29) demonstrated that the Se(0) particles formed by the Se-respiring bacteria Sulfurospirillum barnesii, Bacillus selenitireducens, and Selenihalanaerobacter shriftii are structurally unique compared to elemental selenium formed by chemical synthesis. Furthermore, the Se(0) particles precipitated by these three stra...
The persistence of Shiga toxin-producing E. coli O157:H7 in the environment poses a serious threat to public health. However, the role of Shiga toxins and other virulence factors in the survival of E. coli O157:H7 is poorly defined. The aim of this study was to determine if the virulence factors, stx 1, stx 2, stx 1–2, and eae in E. coli O157:H7 EDL933 play any significant role in the growth of this pathogen in rich media and in soils. Isogenic deletion mutants that were missing one of four virulence factors, stx 1, stx 2, stx 1–2, and eae in E. coli O157:H7 EDL933 were constructed, and their growth in rich media and survival in soils with distinct texture and chemistry were characterized. The survival data were successfully analyzed using Double Weibull model, and the modeling parameters of the mutant strains were not significantly different from those of the wild type. The calculated Td (time needed to reach the detection limit, 100 CFU/g soil) for loamy sand, sandy loam, and silty clay was 32, 80, and 110 days, respectively. It was also found that Td was positively correlated with soil structure (e.g. clay content), and soil chemistry (e.g. total nitrogen, total carbon, and water extractable organic carbon). The results of this study showed that the possession of Shiga toxins and intimin in E. coli O157:H7 might not play any important role in its survival in soils. The double deletion mutant of E. coli O157:H7 (stx 1 − stx 2 −) may be a good substitute to use for the investigation of transport, fate, and survival of E. coli O157:H7 in the environment where the use of pathogenic strains are prohibited by law since the mutants showed the same characteristics in both culture media and environmental samples.
Persistence of Escherichia coli O157:H7 in 32 (16 organically managed and 16 conventionally managed) soils from California (CA) and Arizona (AZ) was investigated. Results showed that the longest survival (ttd, time needed to reach detection limit, 100 CFU g(-1) dry soil) of E. coli O157:H7 was observed in the soils from Salinas Valley, CA and in organically managed soils from AZ. Detrended correspondence analysis revealed that the survival profiles in organically managed soils in Yuma, AZ were different from the ones in conventionally managed soils from the same site. Principal component analysis and stepwise regression analysis showed that E. coli O157:H7 survival in soils was negatively correlated with salinity (EC) (P < 0.001), while positively correlated with assimilable organic carbon (AOC) and total nitrogen (TN) (P < 0.01). Pearson correlation analysis revealed that a greater ttd was associated with a larger δ (time needed for first decimal reduction in E. coli population). EC was negatively correlated and TN was positively correlated (P < 0.05) with δ, suggesting that EC and TN likely have a direct impact on ttd. On the other hand, AOC showed a close correlation with p (the shape parameter) that was not directly related to ttd, indicating that AOC might have an indirect effect in the overall survival of E. coli O157:H7 in soils. Our data showed that AOC and EC significantly affected the survival of E. coli O157:H7 in leafy green producing soils and the development of good agricultural practices (manure/composting/irrigation water source management) in the preharvest environment must be followed to minimize foodborne bacterial contamination on fresh produce.
Shiga toxin-producing Escherichia coli O157:H7 has been implicated in many foodborne illnesses. In this study, survival of E. coli O157:H7 in 32 soils from California (CA) and Arizona (AZ) was investigated. Our goal was to correlate the survival time of E. coli O157:H7 in soils with 16S rRNA pyrosequencing based bacterial community composition. Kohonen self-organizing map of survival and associated soil chemical, physical and biological variables using artificial neural network analysis showed that survival of E. coli O157:H7 in soils was negatively correlated with salinity (EC), but positively correlated with total nitrogen (TN) and water soluble organic carbon (WSOC). Bacterial diversity as determined by the Shannon diversity index had no significant (P = 0.635) effect on ttd, but individual bacterial phyla had different effects. The survival of E. coli O157:H7 was positively correlated with the abundances of Actinobacteria (P < 0.001) and Acidobacteria (P < 0.05), and negatively correlated with those of Proteobacteria and Bacteroidetes (P < 0.05). Our data showed that specific groups of bacteria correlate with the persistence of E. coli O157:H7 in soils thus opening new ways to study the influence of certain bacterial phyla on persistence of this pathogen and other related pathogens in complex environments.
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