RESPON MULTIPLIKASI ANGGREK BULAN  (Phalaenopsis sp.) TERHADAP PENAMBAHAN BEBERAPA KONSENTRASI BAP (Benzyl Amino Purine)  PADA MEDIA IN VITRO

Syamsiah, Melissa; Imansyah, Angga Adriana; Suprapti, Hana Khoerunisa; Badriah, Dedeh Siti

doi:10.35194/agsci.v10i2.1157

Cited by 4 publications

(3 citation statements)

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“…The other study about Phalaenopsis sp. was given BAP cytokinins, with the number of shoots having a substantial impact starting at 4 WAP [19]. Another element that may affect it is that at the age of 10 WAP, a large number of potential shoots are there but cannot be counted since their plumula have not yet pierced the soil surface.…”

Section: 1the Effect Of Explant Types Bap and Naa Combinations And Th...mentioning

confidence: 99%

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Hardiany,

Fauzan,

Pramono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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A medicinal plant called rodent tuber has the potential to both prevent and treat various diseases. There is no Standard Operating Procedure (SOP) governing the growth method of rodent tuber because it still grows wild and is rarely cultivated. This research aimed to study the optimal growth of rodent tuber for mass seed propagation based on the type of explants and the concentration of auxin and cytokinin combinations for inducing shoots. While generative parameters (harvesting of tubers/shoots) were observed up to 13 WAP (Week After Planting), vegetative parameters were observed up to 10 WAP. The results showed that the best type of explants for shoot induction and growth of rodent tuber were whole plants with a survival rate of 100%; the number of shoots was 6.65; the plant height was 21.27 cm with 6.34 leaves; 26.56 roots with a length of 16.05 cm; and 40.80% flowering plants. PGR (Plant Growth Regulator) combination of 50 ppm BAP (Benzyl Amino Purine) and 100 ppm NAA (Naphthalene Acetic Acid) became the best explant soaking solution by forming eight shoots and the height of plants was 24.13 cm with seven leaves. The explant produced the highest root length in a combination of 100 ppm BAP and 50 ppm NAA, which was 17.70 cm. The interaction between whole plant explants immersed in 0 ppm BAP and 100 ppm NAA solution resulted in the most significant number of roots (41). Those goals can support the SOP of propagated methods and ensure the consistency, quality, and availability of raw materials for traditional medicine.

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Section: 1the Effect Of Explant Types Bap and Naa Combinations And Th...mentioning

confidence: 99%

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Hardiany,

Fauzan,

Pramono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Green, pale green, and yellowish-green callus formed SEs whereas yellow and yellowish-green callus turned brown [14]. BAP inhibit chlorophyll degradation and regulate embryonic [37]. BAP is a hormone that can stimulate cell division and induced shoot multiplication [38].…”

Section: Pgrs From Microalgamentioning

confidence: 99%

An Overview: Effect of Plant Growth Regulatory on Orchid Propagation through The Thin Cell Layer technique

Media,

Noli,

Idris

2023

IJPSAT

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Thin cell layer (TCL) is a micropropagation method using thin-sized explants. A TCL can be prepared from any explant source and the thichness of explant less than 5 mm. TCL is more efficient in producing total plantlet output than conventional in vitro methods. TCL have been applied to the in vitro culture of orchids, field, vegetable crops and medicinal plants The successful in vitro orchid propagation is influenced by many factors, such as plant genotype and media composition. Additions of plant growth regulator (PGR) in media culture is essential factor. The formation of complete plant depent on concentration and type of plant growth regulator. TCL explants require growth regulators to form an embryonic callus and zygotic embryos. Explant in medium without supplemented of growth regulators resulted in browning and failed to grow. The most commonly plant growth regulator in tissue culture are thidiazuron (TDZ), 6-benzylaminopurine (BAP) 2,4 dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), and meta-Topolin. Recently, microalgae can also be used as an alternative source of hormones to increase plant growth through in vitro culture techniques

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“…Based on the research results of Syamsiah et al (2020), the addition of BAP with a concentration of 2.00 mg/L was the best treatment with the fastest shoot emergence time of 16.80 DAP (day after planting) and the fastest leaf emergence time of 25.20 DAP and the best number of leaves with an average of 14.4 leaves at the end of the observation, which was a week to 12. Hartati et al (2016), in their research also revealed that the addition of ZPT BAP 3 ppm had a significant effect on the increase in root length and increase in plantlet height, so that increasing the concentration level of BAP could increase the vegetative growth response of orchid explants.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro

Zanirah,

Sutini,

Pribadi

2023

JTEP-L

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The increase in production of Dendrobium bigiante agrihorti orchids by in vitro culture is to produce numerous and uniform seedlings. This study aimed to determine the effect of Growmore and BAP concentrations on the growth of orchid plantlets. The experiment consisted of two factors namely Growmore concentration (0; 3; 6; 9 ppm), and BAP concentrations (0; 1; 3; 5 ppm). The factors combination on ½ MS media was repeated 3 times and obtained 48 experimental units. Observation parameters included the percentage of live plantlets, number of roots, number of leaves, plant height (cm), and wet weight of plantlets (g). Results showed that a Growmore concentration of 3 ppm had a significant effect on the number of roots and plant height at 4.36 units and 4.17 cm. BAP concentration of 3 ppm also had a significant effect on the number of roots, plant height, and wet weight of respectively 4.72 units, 3.22 cm, and 0.63 g. A combination of Growmore 3 ppm + BAP 0 ppm produced the largest percentage of live planets at 100% and plant height at 7.37 cm, Growmore 9 ppm + BAP 0 ppm produced the largest number of roots at 6.33 units and Growmore 6 ppm + BAP 3 ppm produced the largest number of leaves at 8.78 strands. Keywords: BAP, Dendrobium bigiante, Growmore.

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RESPON MULTIPLIKASI ANGGREK BULAN (Phalaenopsis sp.) TERHADAP PENAMBAHAN BEBERAPA KONSENTRASI BAP (Benzyl Amino Purine) PADA MEDIA IN VITRO

Cited by 4 publications

References 0 publications

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

An Overview: Effect of Plant Growth Regulatory on Orchid Propagation through The Thin Cell Layer technique

The Effect of Growmore and BAP (Benzyl Amino Purine) Concentrations on The Growth of Dendrobium bigiante agrihorti Orchid In-Vitro

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