Respiratory system of Fusidium coccineum and regulation of biosynthesis of fusidic acid and sporogenesis

Navashin, S M; Bartoshevich, Yu. E.; Telesnina, G. N.; Zvjagilskaya, R. A.; Dmitriyeva, S. V.; Sazykin, Yu. O.; Krakhmaleva, I. N.

doi:10.1007/bf00499491

Cited by 5 publications

(2 citation statements)

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“…In this study, we cultured FC SA-1FC under conditions that maximized its proliferation after approximately 7 days. In general, the mycelium was well formed and the growth time was 7 days [3]. Also, β-glucogallin was isolated from FC culture medium as a new active compound.…”

Section: Introductionmentioning

confidence: 99%

“…A fungal species belonging to the Ascomycota family, strain SA-1FC was first isolated from soil in 2017. Fusidium coccineum has been widely used as a strain that produces a substance called Fusidic acid in the past, and many studies are focused on the production and analysis of fusidic acid [1][2][3]. However, there are no research results on the effect of Fusidium coccineum on the skin, and in this study, various effects and functional substances of skin cells were screened as metabolites of Fusidium coccineum.…”

Section: Introductionmentioning

confidence: 99%

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β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

Kim

Kim²,

Kim

et al. 2020

Appl Biol Chem

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Soil has been used for treatment of wound and skin diseases and for cosmetic purposes. Fusidium coccineum (FC) SA-1FC (Ascomycota) is a fungus found in nature, and its by-products are present in humid soils with plant humus. This study investigates the medium of fermented FC as a covering for all skin problems, including dryness, inflammation, and wounds. A preliminary study revealed that an alcohol extract of FC had a skin-enhancing effect, and thin-layer chromatography revealed a major component in a non-polar fraction. Here we identify a major compound isolated from a non-polar fraction as β-glucogallin. The mRNA levels of filaggrin and HAS3 are upregulated by FC and β-glucogallin treatment in keratinocytes and immortalized human keratinocytes cells. In addition, FC and β-glucogallin exert anti-inflammatory effects by suppressing expression of interleukin-4/poly(I:C)-induced chemokines and inflammatory cytokines. In fibroblasts, Hs68 cells, FC and β-glucogallin stimulate cell migration. These results suggest that FC and β-glucogallin can enhance skin barrier function.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

Kim

Kim²,

Kim

et al. 2020

Appl Biol Chem

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Bioenergetic parameters of Acremonium chrysogenum

Telesnina¹,

Bartoshevish²,

Krakhmaleva³

et al. 1990

J. Basic Microbiol.

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Bioenergetic parameters of two isogenic strains of Acremonium chrysogenum markedly differing in their capacity for biosynthesis of cephalosporin C were studied. It was shown that in the high productive strain the system of oxidative phosphorylation played the dominant role in the cellular energy supply (the index of the substrate consumption eficiency amounted to 0.82). In both strains terminal oxidation of the reduction equivalents mainly occurred via the respiration chain with cytochrome oxidase as the terminal component. In the low productive strain an alternative cyanide resistant pathway was observed, in addition to the classic cytochrome chain.A procedure suitable for the isolation of stable protoplasts from the mycelium of A . chrysogenum was developed. It was proved to be useful in the isolation of functionally active mitochondria possessing theoretical phosphorylation efficiency and high phosphate acceptor respiration control. It was concluded that there are three oxidative phosphorylation sites in the respiration chain of the high productive strain of A . chrysogenum. . 1981) in Fusidium coccineum, an imperfect fungus, there were peculiar changes in the respiration system were associated with antibiotic production, i.e. there was a shift to the alternative cyanideresistant oxidation pathway. Furthermore, an increase of antibiotic production has been demonstrated by supplementing the cultivation medium with chloramphenicol as an inducer of the cyanide-resistant pathway. Moreover, salicyl hydroxamate as an inhibitor of this pathway inhibited the antibiotic synthesis. Our aim was to demonstrate analogous features in the synthesis of cephalosporin C by Acremonium chrysogenum. Therefore, respiration activities of two isogenic strains of A . chrysogenum distinguished by high and low productivity were investigated. Correlations between bioenergetic processes and antibiotic biosynthesis Materials and methodsStock culture: Two strains of A. chrysogenum were used: the initial strain 226 A and its mutant 12/2 309 A 20 times more active in biosynthesis of cephalosporin C than the initial strain. The strains were maintained on agar containing (g/l) : peptone 0.1, sucrose 0.4 and malt extract 0.1.Submerged cultivation: The inoculum material was grown with intensive aeration in 750 ml flasks containing 50 ml of the complex medium at 28 "C for 3 days. The inoculum (1 per cent of the volume) was added to the fermentation medium consisting of follows (g/l): sucrose 0.6, KH,PO, 0.002, L-asparagine 0.075, . 7 H 2 0 0. I , MnSO, . 4 H20 0.01, pH 6.4. The mycelium was grown in ERLENMEYER flasks (volume 750 ml) containing 50 ml of the medium at 24 "C for 2-6 days depending on the aim of the experiment.18 J.

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Relationship between intracellular pH and antibiotic biosynthesis in Fusidium coccineum

Shipanova

Bartoshevich

Sibel'dina

et al. 1995

Appl Microbiol Biotechnol

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Respiratory system of Fusidium coccineum and regulation of biosynthesis of fusidic acid and sporogenesis

Cited by 5 publications

References 5 publications

β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

Bioenergetic parameters of Acremonium chrysogenum

Relationship between intracellular pH and antibiotic biosynthesis in Fusidium coccineum

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