Relationship between intracellular pH and antibiotic biosynthesis in Fusidium coccineum

Shipanova, I.; Bartoshevich, Yu. E.; Sibel'dina, L A; Zaslavskaya, P. L.; Michtchenko, A.

doi:10.1007/bf00218458

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…This curve has been referred to in many other NMR studies on both yeasts and fungi [16,20,21], and shows the pH dependence of the chemical shift of P i measured in a medium made up to approximate concentrations of the major cationic components in yeast [22]. The perfusion buffer pH (pH ex ) was monitored by pH electrode measurements.…”

Section: Analysesmentioning

confidence: 89%

“…Cytoplasmic and vacuolar pH values were determined by comparing the pH‐sensitive chemical shifts of cytoplasmic ( P cyt ) and vacuolar inorganic phosphate ( P vac ) with a calibration curve for inorganic phosphate (P i ). This curve has been referred to in many other NMR studies on both yeasts and fungi [16,20,21], and shows the pH dependence of the chemical shift of P i measured in a medium made up to approximate concentrations of the major cationic components in yeast [22]. The perfusion buffer pH (pH ex ) was monitored by pH electrode measurements.…”

Section: Methodsmentioning

confidence: 98%

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Intracellular pH homeostasis in the filamentous fungus Aspergillus niger

Hesse

Ruijter

Dijkema

et al. 2002

European Journal of Biochemistry

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Intracellular pH homeostasis in the filamentous fungus Aspergillus niger was measured in real time by 31 P NMR during perfusion in the NMR tube of fungal biomass immobilized in Ca 2+ -alginate beads. The fungus maintained constant cytoplasmic pH (pH cyt ) and vacuolar pH (pH vac ) values of 7.6 and 6.2, respectively, when the extracellular pH (pH ex ) was varied between 1.5 and 7.0 in the presence of citrate. Intracellular metabolism did not collapse until a DpH over the cytoplasmic membrane of 6.6-6.7 was reached (pH ex 0.7-0.8). Maintenance of these large pH differences was possible without increased respiration compared to pH ex 5.8. Perfusion in the presence of various hexoses and pentoses (pH ex 5.8) revealed that the magnitude of DpH values over the cytoplasmic and vacuolar membrane could be linked to the carbon catabolite repressing properties of the carbon source. Also, larger DpH values coincided with a higher degree of respiration and increased accumulation of polyphosphate. Addition of protonophore (carbonyl cyanide m-chlorophenylhydrazone, CCCP) to the perfusion buffer led to decreased ATP levels, increased respiration and a partial (1 lM CCCP), transient (2 lM CCCP) or permanent (10 lM CCCP) collapse of the vacuolar membrane DpH. Nonlethal levels of the metabolic inhibitor azide (N 3 -, 0.1 mM) caused a transient decrease in pH cyt that was closely paralleled by a transient vacuolar acidification. Vacuolar H + influx in response to cytoplasmic acidification, also observed during extreme medium acidification, indicates a role in pH homeostasis for this organelle. Finally, 31 P NMR spectra of citric acid producing A. niger mycelium showed that despite a combination of low pH ex (1.8) and a high acidsecreting capacity, pH cyt and pH vac values were still well maintained (pH 7.5 and 6.4, respectively).

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Section: Analysesmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 98%

Intracellular pH homeostasis in the filamentous fungus Aspergillus niger

Hesse

Ruijter

Dijkema

et al. 2002

European Journal of Biochemistry

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“…Cytoplasmic and vacuolar pH values of A. niger were determined by comparing the pH-sensitive chemical shifts of cytoplasmic and vacuolar inorganic phosphate resonance (P cyt and P vac , respectively), using a calibration curve for inorganic phosphate (P i ) (10), as utilized in previous work (24,27,38). The pH of the perfusion buffer was determined using a pH electrode.…”

Section: Methodsmentioning

confidence: 99%

The Weak Acid Preservative Sorbic Acid Inhibits Conidial Germination and Mycelial Growth of Aspergillus niger through Intracellular Acidification

Plumridge

Hesse

Watson

et al. 2004

Appl Environ Microbiol

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The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10 5 /ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h developed from the same spore inoculum was threefold lower. The MIC for conidia and, to a lesser extent, mycelia was shown to be dependent on the inoculum size. A. niger is capable of degrading sorbic acid, and this ability has consequences for food preservation strategies. The mechanism of action of sorbic acid was investigated using 31 P nuclear magnetic resonance (NMR) spectroscopy. We show that a rapid decline in cytosolic pH (pH cyt ) by more than 1 pH unit and a depression of vacuolar pH (pH vac ) in A. niger occurs in the presence of sorbic acid. The pH gradient over the vacuole completely collapsed as a result of the decline in pH cyt . NMR spectra also revealed that sorbic acid (3.0 mM at pH 4.0) caused intracellular ATP pools and levels of sugar-phosphomonoesters and -phosphodiesters of A. niger mycelia to decrease dramatically, and they did not recover. The disruption of pH homeostasis by sorbic acid at concentrations below the MIC could account for the delay in spore germination and retardation of the onset of subsequent mycelial growth.

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“…A fungal species belonging to the Ascomycota family, strain SA-1FC was first isolated from soil in 2017. Fusidium coccineum has been widely used as a strain that produces a substance called Fusidic acid in the past, and many studies are focused on the production and analysis of fusidic acid [1][2][3]. However, there are no research results on the effect of Fusidium coccineum on the skin, and in this study, various effects and functional substances of skin cells were screened as metabolites of Fusidium coccineum.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, effective substances were found for skin cell regeneration-recovery-soothingmoisturizing, etc., and results were derived for efficacy and material properties. Although FC can produce antibiotics, such as fusidic acid for inhibiting skin infection, special processes are required to extract these products [1,2]. In this study, we cultured FC SA-1FC under conditions that maximized its proliferation after approximately 7 days.…”

Section: Introductionmentioning

confidence: 99%

β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

Kim

Kim²,

Kim

et al. 2020

Appl Biol Chem

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Soil has been used for treatment of wound and skin diseases and for cosmetic purposes. Fusidium coccineum (FC) SA-1FC (Ascomycota) is a fungus found in nature, and its by-products are present in humid soils with plant humus. This study investigates the medium of fermented FC as a covering for all skin problems, including dryness, inflammation, and wounds. A preliminary study revealed that an alcohol extract of FC had a skin-enhancing effect, and thin-layer chromatography revealed a major component in a non-polar fraction. Here we identify a major compound isolated from a non-polar fraction as β-glucogallin. The mRNA levels of filaggrin and HAS3 are upregulated by FC and β-glucogallin treatment in keratinocytes and immortalized human keratinocytes cells. In addition, FC and β-glucogallin exert anti-inflammatory effects by suppressing expression of interleukin-4/poly(I:C)-induced chemokines and inflammatory cytokines. In fibroblasts, Hs68 cells, FC and β-glucogallin stimulate cell migration. These results suggest that FC and β-glucogallin can enhance skin barrier function.

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Relationship between intracellular pH and antibiotic biosynthesis in Fusidium coccineum

Cited by 8 publications

References 7 publications

Intracellular pH homeostasis in the filamentous fungus Aspergillus niger

Intracellular pH homeostasis in the filamentous fungus Aspergillus niger

The Weak Acid Preservative Sorbic Acid Inhibits Conidial Germination and Mycelial Growth of Aspergillus niger through Intracellular Acidification

β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects

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