Resolution of ( RS )-Proglumide using Lipase from Candida cylindraceae

Muralidhar, R. V.; Chirumamilla, RR; Ramachandran, V. N.; Marchant, Roger; Nigam, Poonam Singh Nee

doi:10.1016/s0968-0896(01)00409-6

Cited by 17 publications

(6 citation statements)

References 15 publications

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“…Lipases have been widely studied for their properties and utilization in many industries [71,72,73,74,75]. Pectinases have established their role in the fruit and juice industries [76].…”

Section: Enzymes With Special Characteristics In Biotechnologymentioning

confidence: 99%

Microbial Enzymes with Special Characteristics for Biotechnological Applications

2013

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This article overviews the enzymes produced by microorganisms, which have been extensively studied worldwide for their isolation, purification and characterization of their specific properties. Researchers have isolated specific microorganisms from extreme sources under extreme culture conditions, with the objective that such isolated microbes would possess the capability to bio-synthesize special enzymes. Various Bio-industries require enzymes possessing special characteristics for their applications in processing of substrates and raw materials. The microbial enzymes act as bio-catalysts to perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. The special characteristics of enzymes are exploited for their commercial interest and industrial applications, which include: thermotolerance, thermophilic nature, tolerance to a varied range of pH, stability of enzyme activity over a range of temperature and pH, and other harsh reaction conditions. Such enzymes have proven their utility in bio-industries such as food, leather, textiles, animal feed, and in bio-conversions and bio-remediations.

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“…Lipases have been widely studied for their properties and utilization in many industries [71,72,73,74,75]. Pectinases have established their role in the fruit and juice industries [76].…”

Section: Enzymes With Special Characteristics In Biotechnologymentioning

confidence: 99%

Microbial Enzymes with Special Characteristics for Biotechnological Applications

2013

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“…Hydrolase enzymes are gaining attention because of their wider utilisation as biocatalysts. Esterases and lipases are two major classes of hydrolases, but both share certain common features, for example, the racemic resolution of compounds, however, they also have their own unique properties [11,12]. The three-dimensional structure of both enzymes shows a characteristic-hydrolase fold [13,14].…”

Section: Hydrolase Enzymesmentioning

confidence: 99%

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

Dahiya

Nigam

2022

Sustainability

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Reactions catalysed by sustainably produced enzymes can contribute to the bioeconomy supporting several industries. Low-value compounds can be transformed into added-value products or high-resolution chemicals could be prepared in reactions catalysed by biocatalyst esterase enzymes. These enzymes can be synthesised by purposely isolated or genetically modified strains of microorganisms. Enzymes belonging to the hydrolase family catalyse the formation and hydrolysis of ester bonds to produce the desired esterified molecule. The synthesis of homo-chiral compounds can be accomplished either by chemical or biocatalytic processes, the latter being preferred with the use of microbial esterases. For varied applications, esterases with high stability and retained activity at lower and higher temperatures have been produced with strains isolated from extreme environments. For sustainable production of enzymes, higher productivity has been achieved by employing fast-growing Escherichia coli after incorporating plasmids of required characteristics from specific isolates. This is a review of the isolated and engineered strains used in the biosynthesis of esterase of the desired property, with the objective of a sustainable supply of enzymes, to produce products of industrial importance contributing to the economy.

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“…The P-value was used to verify the significance of each coefficient and to describe the degree of interactions strength between the variables (Muralidhar et al, 2001). A smaller P-value means the corresponding coefficient is more significant (Muralidhar et al, 2001).…”

Section: Optimization Of Extraction Yield and Purity Of Tdfmentioning

confidence: 99%

Optimization of processing parameters for extraction of total, insoluble and<br>soluble dietary fibers of defatted rice bran

Daou

Zhang

2013

Emir. J. Food Agric

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Response surface methodology was used to optimize the processing parameters for extraction of total dietary fiber (TDF), insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) of defatted rice bran (DRB). The studied independent factors were concentration of NaOH solution (varying from 0.15 to 0.25 mol/L), soaking time (from 60 to 90 min), α-amylase enzyme -substrate (E: S) ratio (from 0.6:100 to 0.9:100 g: g of dry DRB) and alcalase enzyme concentration (from 3.5:100 to 4.7 g: g of dry DRB)) whereas; the dependent variables were extraction yield and purity of TDF, IDF and SDF. Therefore, the three-level four-factor Box-Behnken design was used to establish the optimum conditions and the generated regression quadratic polynomial models and adequacy of each dependent variable were significant (p < 0.0001) with regression coefficient R2 (> 0.90) and lack of-fit was not significant. Moreover, ANOVA showed that most of the linear, interaction and quadratic regression coefficient values were significant (p < 0.05). The optimum processing parameters observed for extraction of TDF, IDF and SDF with high yield and purity were: 0.15 mol/L NaOH solution concentration, 64.3 min soaking time, 0.68:100 and 3.52:100 (g: g) α-amylase and alcalase enzyme -substrate ratio (E: S), respectively. Moreover, the alkali pretreatment was the factor amongst the others that significantly (p<0.05) affected the purity of Fiber fractions but did not contribute to improve their yields.

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Resolution of ( RS )-Proglumide using Lipase from Candida cylindraceae

Cited by 17 publications

References 15 publications

Microbial Enzymes with Special Characteristics for Biotechnological Applications

Microbial Enzymes with Special Characteristics for Biotechnological Applications

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

Optimization of processing parameters for extraction of total, insoluble and<br>soluble dietary fibers of defatted rice bran

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