Resistome of a carbapenemase-producing novel ST232 Klebsiella michiganensis isolate from urban hospital effluent in South Africa

King, Tracy; Schmidt, Štefan; Thakur, Siddhartha; Fedorka-Cray, Paula J.; Keelara, Shivaramu; Harden, Lyndy; Essack, Sabiha Y.

doi:10.1016/j.jgar.2021.01.004

Cited by 8 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial adherence to and invasion of host tissue and cells may increase the pathogenicity of bacterial infections, such as urinary tract infections and infective endocarditis [ 35 ]. The predicted defensive virulence genes were mainly involved in the production of stress proteins and in anti-phagocytosis processes, and in capsule biosynthesis to evade the host immune system ( Table 1 ) [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

et al. 2021

View full text Add to dashboard Cite

In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.

show abstract

Section: Resultsmentioning

confidence: 99%

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The geographic distribution showed that K. michiganensis strains were mainly distributed in North America, Europe, East Asia, and Oceania ( Figure 1A ). Although K. michiganensis had also been reported in South Africa before, 6 , 33 the complete genome was not available in the NCBI Genome database, so K. michiganensis reported in South Africa was not included in this study. Sixteen of these strains originated in China.…”

Section: Resultsmentioning

confidence: 99%

“…Since first being discovered in 2012, K. michiganensis has caused several serious and difficult-to-treat nosocomial infections. 2–4 , 6–8 IncHI5 plasmids, a subgroup of IncHI, usually carry a variety of MGEs and drug resistance genes according to previous reports. 9 , 12 , 40 , 41 The K. michiganensis carrying IncHI5 plasmids were identified frequently in the last two years.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Klebsiella michiganensis Carrying Multidrug-Resistant IncHI5 Plasmids in the Southeast Coastal Area of China

Luo

Zhang

Yuan

et al. 2022

IDR

View full text Add to dashboard Cite

Purpose This study aimed to explore the genomic characterization of multidrug-resistant IncHI5-carrying Klebsiella michiganensis strains and detailed genomic dissection of the IncHI5 plasmids. Materials and Methods Through whole-genome sequencing, the IncHI5 plasmid pK92-qnrS was obtained from a single clinical K. michiganensis isolate K92. All complete genomes of K. michiganensis strains from the Genome database of NCBI were collected and used to construct a maximum likelihood (ML) phylogenetic tree. The epidemiology and geographic distribution of all the K. michiganensis strains were conducted. An extensive comparison of the seven IncHI5 plasmids of K. michiganensis (one from this study, six from GenBank) was applied. Results This study revealed that all K. michiganensis strains carrying IncHI5 plasmids from different clonal groups were located in the southeast coastal area of China. The backbone regions of IncHI5 plasmids were composed of replicon ( repHI5B and repFIB ), partition ( parABC ), and conjugal transfer ( tra1 / tra2 ). The main accessory resistant regions of IncHI5 could be divided into two categories, Tn 1696 -related region and Tn 6535 -related region. These seven IncHI5 plasmids carried multiple drug-resistance genes which were all mediated by the mobile genetic elements (MGEs). Conclusion Data presented here help to provide an overall in-depth understanding of epidemiology and geographic distribution of IncHI5-carrying K. michiganensis and the structure and evolutionary history of IncHI5 plasmids.

show abstract

“…K. michiganensis is a multidrug resistant bacteria harboring (KPC)-3 carbapenemase gene, carbapenemase gene blaOXA-181 and other 11 genes encoding different antibiotic resistance. The carbapenemase gene encoding plasmid is originated from Klebsiella pneumoniae [60,61]. Mitra et al [62].…”

Section: Occurrence Of Allochthonous Microorganisms In Traditionally Processed Cheesementioning

confidence: 99%

Microbial Diversity of Traditionally Processed Cheese from Northeastern Region of Transylvania (Romania)

György¹,

Laslo²

2021

Fermentation - Processes, Benefits and Risks

View full text Add to dashboard Cite

The composition and production technology of the cheese are extremely diverse. There are a wide variety of microbial species on their surface, with a much smaller number inside of the product. The microbiota of the cheese may be composed of beneficial microorganisms, spoilage and foodborne pathogens. Identification and characterization of the microorganisms present in these products are important nutrition, food safety and technological aspects. During our work we evaluated the prevalence of allochthonous bacteria and microscopic fungi in traditionally processed cheeses from northeastern region of Transylvania, with classical microbiological culture methods. Based on the results the microbiota of the analysed cheeses was highly diversified. The identified bacteria with the highest prevalence from different selective media, were as follows: Escherichia coli, Enterococcus durans, Enterococcus faecalis, Shigella flexnerii, Proteus vulgaris, Stenotrophomonas maltophilia, Staphylococcus equorum subsp. equorum, Staphylococcus equorum subsp. linens, Halomonas alkaliphila, Kocuria rhizophila, Hafnia paralvei, Bacillus licheniformis and Klebsiella michiganensis.

show abstract

Resistome of a carbapenemase-producing novel ST232 Klebsiella michiganensis isolate from urban hospital effluent in South Africa

Cited by 8 publications

References 14 publications

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

Epidemiology of Klebsiella michiganensis Carrying Multidrug-Resistant IncHI5 Plasmids in the Southeast Coastal Area of China

Microbial Diversity of Traditionally Processed Cheese from Northeastern Region of Transylvania (Romania)

Contact Info

Product

Resources

About