A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

Abstract

In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.

Safety Assessment of One Lactiplantibacillus plantarum Isolated from the Traditional Chinese Fermented Vegetables—Jiangshui

“…Red blood cells and hemolysin (an immunogenic novel toxin with hemolytic), when activated by complement, can dissolve the antibody and then damage the red blood cells, resulting in a cascade of events such as anemia [ 27 , 28 ]. To determine the type of hemolysis, blood agar plates were used.…”

“…During the entire treatment, there was no effect of oral administration on the body weight and mortality in both control and experimental groups. In preceding studies, the feeding of 10 10 CFU/mL of LAB isolates did not influence body weight, general signs, and food consumption during the acute oral toxicity testing [ 28 , 36 ]. The serum biochemistry results in Table 3 showed that all groups exhibited normal ranges of hematological parameters [ 37 ].…”

Blautia producta displays potential probiotic properties against dextran sulfate sodium-induced colitis in mice

“…Previous studies exhibited that probiotics have promising potential in reducing the incidence and mortality of colitis [7,8]. Blautia is widely distributed in the feces and intestines of mammals and has attracted growing interest due to its potential correlations with alleviation of metabolic and inflammatory diseases, and inhibition of specific bacterial growth [9][10][11]. Previous studies revealed that the abundance of Blautia is positively associated with the improvement of lipid metabolism [12] and the protein and gene expressions of ZO-1 and occluding [13].…”

The Characterization of a Novel D-allulose 3-Epimerase from Blautia produca and Its Application in D-allulose Production

“…The human gut bacteria Blautia produca isolated from feces whose species belonging to the Lachnospiraceae family had rapidly attracted more attention for its capabilities to alleviate inflammatory, metabolic disorders and its antibacterial properties against particular microorganisms since its discovery [ 44 , 45 ]. It could be used as a probiotic in food, medicinal and culinary preparations [ 46 ].…”