Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation

Osuna, M. D.; Goulart, Ives Clayton Gomes dos Reis; Vidal, Ribas Antônio; Kalsing, Augusto; Santaella, J.P. Ruiz

doi:10.1590/s0100-83582012000300025

Cited by 21 publications

(41 citation statements)

References 19 publications

(17 reference statements)

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“…Contrary to earlier classifications, numerous recent investigations have clearly shown that, with the exception of the D2078G and C2088R mutations that confer broad resistance to all herbicides tested, the levels of resistance depend on specific amino acid changes, the number of resistant alleles, weed species, plant growth stages and recommended field rates of herbicides. For instance, plants with the I2041N were found to be sensitive to cycloxydim in A. myosuroides but resistant in P. paradoxa.…”

Section: Mechanism Of Resistancecontrasting

confidence: 64%

Resistance to acetyl‐CoA carboxylase‐inhibiting herbicides

Kaundun

2014

Pest Management Science

196

222

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Resistance to acetyl-CoA carboxylase herbicides is documented in at least 43 grass weeds and is particularly problematic in Lolium, Alopecurus and Avena species. Genetic studies have shown that resistance generally evolves independently and can be conferred by target-site mutations at ACCase codon positions 1781, 1999, 2027, 2041, 2078, 2088 and 2096. The level of resistance depends on the herbicides, recommended field rates, weed species, plant growth stages, specific amino acid changes and the number of gene copies and mutant ACCase alleles. Non-target-site resistance, or in essence metabolic resistance, is prevalent, multigenic and favoured under low-dose selection. Metabolic resistance can be specific but also broad, affecting other modes of action. Some target-site and metabolic-resistant biotypes are characterised by a fitness penalty. However, the significance for resistance regression in the absence of ACCase herbicides is yet to be determined over a practical timeframe. More recently, a fitness benefit has been reported in some populations containing the I1781L mutation in terms of vegetative and reproductive outputs and delayed germination. Several DNA-based methods have been developed to detect known ACCase resistance mutations, unlike metabolic resistance, as the genes remain elusive to date. Therefore, confirmation of resistance is still carried out via whole-plant herbicide bioassays. A growing number of monocotyledonous crops have been engineered to resist ACCase herbicides, thus increasing the options for grass weed control. While the science of ACCase herbicide resistance has progressed significantly over the past 10 years, several avenues provided in the present review remain to be explored for a better understanding of resistance to this important mode of action.

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Section: Mechanism Of Resistancecontrasting

confidence: 64%

Resistance to acetyl‐CoA carboxylase‐inhibiting herbicides

Kaundun

2014

Pest Management Science

196

222

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“…The plastidic ACCase gene sequences from a total of nine individual plants surviving fluazifop‐P‐butyl or butroxydim were analysed in comparison with those of the susceptible plants. The primer pair ELEIN1781F/ELEIN1781R (Osuna et al ., ) amplified a 600 bp DNA fragment covering the known mutation site 1781, and the primer pair ELEIN2027f/ELEIN2027r amplified an 832 bp fragment with the known mutation sites 1999, 2027, 2041, 2078, 2088 and 2096. Sequence alignment revealed an amino acid substitution of Trp‐2027‐Cys in R individuals, resulting from a G to T change at the third position of the Trp codon (TGG).…”

Section: Resultsmentioning

confidence: 99%

“…(). Published primers (Osuna et al ., ) used to amplify two plastidic ACCase gene fragments in which point mutations known to confer ACCase herbicide resistance in plants have been identified (Délye & Michel, ; Powles & Yu, ; Beckie & Tardif, ). The PCR was conducted in a 25 μL volume that consisted of 1–2 μL containing 50–100 ng of genomic DNA, 0.5 μ m of each primer and 12.5 μL of 2× GoTaq Green Master Mix ® (Promega, Madison, WI, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Control of E. indica is mainly with herbicides, but over‐reliance on herbicides has resulted in resistance evolution in this species in at least eight countries (Heap, ). This includes resistance to dinitroaniline herbicides (Mudge et al ., ), acetyl coA carboxylase (ACCase)‐inhibiting herbicides (Leach et al ., ; Osuna et al ., ), the acetolactate synthase (ALS)‐inhibiting herbicide imazapyr (Valverde et al ., ), the glycine herbicide glyphosate (Lee & Ngim, ), the bipyridilium herbicide paraquat (Buker et al ., ), photosystem II inhibitors (Brosnan et al ., ), and most recently, the glutamine synthetase‐inhibiting herbicide glufosinate (Chuah et al ., ; Jalaludin et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Multiple resistance across glufosinate, glyphosate, paraquat and ACCase‐inhibiting herbicides in an Eleusine indica population

Jalaludin

Powles

2014

Weed Research

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An Eleusine indica population was previously reported as the first global case of field-evolved glufosinate resistance. This study re-examines glufosinate resistance and investigates multiple resistance to other herbicides in the population. Dose-response experiments with glufosinate showed that the resistant population is 5-fold and 14-fold resistant relative to the susceptible population, based on GR 50 and LD 50 R/S ratio respectively. The selected glufosinate-resistant subpopulation also displayed a high-level resistance to glyphosate, with the respective GR 50 and LD 50 R/S ratios being 12-and 144-fold. In addition, the subpopulation also displayed a level of resistance to paraquat and ACCase-inhibiting herbicides fluazifop-P-butyl, haloxyfop-P-methyl and butroxydim. ACCase gene sequencing revealed that the Trp-2027-Cys mutation is likely responsible for resistance to the ACCase inhibitors examined. Here, we confirm glufosinate resistance and importantly, we find very high-level glyphosate resistance, as well as resistance to paraquat and ACCase-inhibiting herbicides. This is the first confirmed report of a weed species that evolved multiple resistance across all the three non-selective global herbicides, glufosinate, glyphosate and paraquat.

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“…Fluazifop Haloxyfop Cyhalofop Sethoxydim Clethodim Tepraloxydim (Powles and Yu, 2010;Osuna et al, 2012;Kukorelli et al, 2013) Cys2088Arg…”

Section: Eleusine Indicamentioning

confidence: 99%

ACCase-inhibiting herbicides: mechanism of action, resistance evolution and stewardship

Takano

Ovejero

Belchior³

et al. 2021

Sci. agric. (Piracicaba, Braz.)

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Herbicides play an important role in preventing crop yield losses due to both their weed interference ability and their capacity for increasing soil conservation in no-till systems. Group A herbicides or acetyl-CoA carboxylase (ACCase) are essential tools the selective management of glyphosate resistance in grass weed species. In this review, we describe important aspects of ACCase biology and herbicides targeting this enzyme, along with a discussion on stewardship programs to delay the evolution of herbicide resistance which can evolve either through target site and/or non-target site mechanisms. Sixteen-point mutations have been reported to confer resistance to ACCase inhibitors. Each mutation confers cross resistance to a different group of herbicides. Metabolic resistance can result in resistance to multiple herbicides with different mechanisms of action (MoA), and herbicide detoxification is often conferred by cytochrome P450 monooxigenases and glutathione-Stransferases. Regardless of whether resistance mechanisms are target or non-target site, using herbicides with the same MoA will result in resistance evolution. Therefore, while field surveys and resistance mechanism studies are crucial for designing reactive management strategies, integrated weed management plays a central role in both reactive and proactive mitigation of herbicide resistance evolution.

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Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation

Cited by 21 publications

References 19 publications

Resistance to acetyl‐CoA carboxylase‐inhibiting herbicides

Resistance to acetyl‐CoA carboxylase‐inhibiting herbicides

Multiple resistance across glufosinate, glyphosate, paraquat and ACCase‐inhibiting herbicides in an Eleusine indica population

ACCase-inhibiting herbicides: mechanism of action, resistance evolution and stewardship

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