Resistance rate and novel virulence factor determinants of Arcobacter spp., from cattle fresh meat products from Iraq

Jasim, Saade Abdalkareem; Al-Abodi, Hiba Riyadh; Ali, Wala’a Shawkat

doi:10.1016/j.micpath.2020.104649

Cited by 19 publications

(11 citation statements)

References 31 publications

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“…In contrast to our findings, the prevalence of Arcobacter isolated from chicken meat was higher than the prevalence found in Iran (26%) (Khodamoradi and Abiri, 2020), Germany (26.8%) (Lehmann et al, 2015), and Tunisia (13.42%) (Jribi et al, 2020), but similar to that found in Beijing (73.33%) (Wang et al, 2016). Jasim et al (2021) reported that (120/1,293) the prevalence of positive beef samples in Iraq was 9.25%, which was lower than that in the present study. Mottola et al (2021) reported that its prevalence in ready-to-eat vegetables was 14.5% (16/110), which was lower than that in the present…”

Section: Discussioncontrasting

confidence: 90%

“…The prevalence of Arcobacter in broiler chickens was reported as 26.0% (26/100) in Iran (Khodamoradi and Abiri, 2020 ), 55.7% (54/97) in the south of Chile (Vidal-Veuthey et al, 2021 ), and 73.33% (44/60) in Beijing, China (Wang et al, 2016 ). Moreover, Arcobacter is also found in beef, pork, vegetables, and seafood (Mottola et al, 2016 , 2021 ; Córdoba-Calderón et al, 2017 ; Kim et al, 2019 ; Zhang et al, 2019 ; Jasim et al, 2021 ), which represent possible transmission sources to humans. However, there have been a few reports regarding Arcobacter isolated from various sources in China.…”

Section: Introductionmentioning

confidence: 99%

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Genetic characteristics, antimicrobial resistance, and prevalence of Arcobacter spp. isolated from various sources in Shenzhen, China

Zhou

et al. 2022

Front. Microbiol.

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Arcobacter spp. is a globally emerging zoonotic and foodborne pathogen. However, little is known about its prevalence and antimicrobial resistance in China. To investigate the prevalence of Arcobacter spp. isolated from various sources, 396 samples were collected from human feces, chicken cecum, and food specimens including chicken meat, beef, pork, lettuce, and seafood. Arcobacter spp. was isolated by the membrane filtration method. For 92 strains, the agar dilution method and next-generation sequencing were used to investigate their antimicrobial resistance and to obtain whole genome data, respectively. The virulence factor database (VFDB) was queried to identify virulence genes. ResFinder and the Comprehensive Antibiotic Resistance Database (CARD) were used to predict resistance genes. A phylogenetic tree was constructed using the maximum likelihood (ML) method with core single-nucleotide polymorphisms (SNPs). We found that 27.5% of the samples (n = 109) were positive for Arcobacter spp., comprising Arcobacter butzleri (53.0%), Arcobacter cryaerophilus (39.6%), and Arcobacter skirrowii (7.4%). Chicken meat had the highest prevalence (81.2%), followed by seafood (51.9%), pork (43.3%), beef (36.7%), lettuce (35.5%), chicken cecum (8%), and human fecal samples (0%, 0/159). Antimicrobial susceptibility tests revealed that 51 A. butzleri and 40 A. cryaerophilus strains were resistant to streptomycin (98.1, 70%), clindamycin (94.1, 90%), tetracycline (64.7, 52.5%), azithromycin (43.1%, 15%), nalidixic acid (33.4, 35%), and ciprofloxacin (31.3, 35%) but were susceptible to erythromycin, gentamicin, chloramphenicol, telithromycin, and clindamycin (≤10%). A. skirrowii was sensitive to all experimental antibiotics. The virulence factors tlyA, mviN, cj1349, ciaB, and pldA were carried by all Arcobacter spp. strains at 100%, and the following percentages were cadF (95.7%), iroE (23.9%), hecB (2.2%), hecA, and irgA (1.1%). Only one A. butzleri strain (F061-2G) carried a macrolide resistance gene (ereA). One A. butzleri and one A. cryaerophilus harbored resistance island gene clusters, which were isolated from pork and chicken. Phylogenetic tree analysis revealed that A. butzleri, A. cryaerophilus, and A. skirrowii were separated from each other. To our knowledge, this is the first report of the isolation of Arcobacter spp. from vegetables and seafood in China. The resistance island gene cluster found in pork and chicken meat and the presence of virulence factors could be a potential risk to human health.

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Section: Discussioncontrasting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Genetic characteristics, antimicrobial resistance, and prevalence of Arcobacter spp. isolated from various sources in Shenzhen, China

Zhou

et al. 2022

Front. Microbiol.

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“…To our knowledge, this is the first study to report the presence of A. butzleri in beef liver. Its occurrence is higher in this by-product than what has been reported for retail beef meat in Asian and European countries (Kabeya et al 2004;Scullion et al 2006;De Smet et al 2010;Shah et al 2012;Zacharow et al 2015;Jasim et al 2020). This is an emerging pathogen that has been linked to both gastrointestinal and systemic diseases in humans and whose routes of transmission are still not entirely clear (Mateus et al 2021).…”

Section: Resultsmentioning

confidence: 94%

“…2015; Jasim et al . 2020). This is an emerging pathogen that has been linked to both gastrointestinal and systemic diseases in humans and whose routes of transmission are still not entirely clear (Mateus et al .…”

Section: Resultsmentioning

confidence: 99%

Campylobacter jejuni and other emerging Campylobacteraceae in retail beef liver – an underestimated potential source?

Lopez-Cantillo

Opazo-Capurro

López-Joven

et al. 2022

Letters in Applied Microbiology

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Bovine by‐products, such as liver, could be an underestimated source of Campylobacter jejuni. Therefore, our aims were to evaluate the occurrence of C. jejuni and other Campylobacteraceae in retail beef liver and characterize their antibiotic resistance (ciprofloxacin, tetracycline, erythromycin and gentamicin) and potential genetic relationship by flagellin gene restriction fragment length polymorphism (flaA‐RFLP) and multilocus sequence typing with clinical strains. Seventy‐six out of 206 samples (36·9%) were positive for Campylobacter and related organisms. Arcobacter butzleri was the most frequently isolated species (21·8%), followed by C. jejuni (9·7%), C. fetus (7·8%) and C. coli (1%). The C. jejuni strains showed resistance to tetracycline (17·2%) or ciprofloxacin (6·9%), with only one strain resistant to both antibiotics. Meanwhile, 8·3% of ciprofloxacin resistance was observed in C. fetus. The other species showed no resistance. Most of the clonal complexes (CC) in which the C. jejuni genotypes were grouped (CC‐21, 42, 48 and 52), coincided with genotypes of clinical strains previously reported in Chile. As such, this study provides evidence that beef liver could be an underestimated route for resistant C. jejuni to humans. Further studies should assess whether this food could play a role in the transmission of other emerging Campylobacteraceae such as those reported here.

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“…( Ferreira et al., 2018 ). Multidrug resistance in Arcobacter is scarcely reported but sporadic reduced susceptibility to antibiotics has been observed by several studies ( Ferreira et al., 2013 ; Millar and Raghavan, 2017 ; Šilha et al., 2017 ; Isidro et al., 2020 ; Jribi et al., 2020 ; Jasim et al., 2021 ; Sciortino et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Arcobacter butzleri isolates from poultry in rural Ghana

Zautner

Riedel²,

Bunk³

et al. 2023

Front. Cell. Infect. Microbiol.

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In recent years, Arcobacter butzleri has gained clinical significance as an emerging diarrheagenic pathogen associated with poultry and water reservoirs. The full clinical significance of Arcobacter remains rather speculative due to variable virulence and antibiotic susceptibility of individual strains. The aims of the present study were (i) to identify antibiotic resistance genes (ARGs) in the genome sequences of two multidrug-resistant A. butzleri isolates, (ii) to use multilocus-sequence typing (MLST) to generate a guiding phylogeny of A. butzleri isolates collected in Kumasi, Ghana, (iii) to examine the distribution of ARGs in the test cohort, and (iv) to assess the strain’s virulence and possible antibiotic treatment options for arcobacteriosis based on the genome sequences and the ARG distribution. A total of 48 A. butzleri isolates obtained from poultry were included in the analysis. These isolates were genotyped by MLST and the antibiotic susceptibilities of isolates to ampicillin, ciprofloxacin, tetracycline, gentamicin, and erythromycin were tested by disk diffusion. Whole genome sequence data of two multidrug-resistant (MDR) A. butzleri isolates were obtained by a combination of single-molecule real-time (SMRT) and Illumina sequencing technology. A total of 14 ARGs were identified in the two generated genome sequences. For all 48 isolates, the frequency of these 14 ARGs was investigated by PCR or amplicon sequencing. With 44 different sequence types found among 48 isolates, strains were phylogenetically heterogeneous. Four of 48 isolates showed an ARG constellation indicating a multidrug-resistant phenotype. The virulence genes in the two A. butzleri genomes showed that the species might be characterized by a somewhat lower virulence as Campylobacter species. The phenotypic susceptibility data combined with the distribution of the particular ARGs especially oxa-464 and the T81I point mutation of the quinolone resistance determining region (QRDR) in a significant percentage of isolates indicated that macrolides and tetracycline can be recommended for calculated antibiotic treatment of arcobacteriosis in Ghana, but not ampicillin and quinolones.

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Resistance rate and novel virulence factor determinants of Arcobacter spp., from cattle fresh meat products from Iraq

Cited by 19 publications

References 31 publications

Genetic characteristics, antimicrobial resistance, and prevalence of Arcobacter spp. isolated from various sources in Shenzhen, China

Genetic characteristics, antimicrobial resistance, and prevalence of Arcobacter spp. isolated from various sources in Shenzhen, China

Campylobacter jejuni and other emerging Campylobacteraceae in retail beef liver – an underestimated potential source?

Molecular characterization of Arcobacter butzleri isolates from poultry in rural Ghana

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