Resistance mechanisms and associated mutations in acetylcholinesterase genes in Sitobion avenae (Fabricius)

Chen, Maohua; Han, Zhaojun; Qiao, Xianfeng; Qu, Mingjing

doi:10.1016/j.pestbp.2006.07.009

Cited by 44 publications

(31 citation statements)

References 18 publications

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“…In A. gossypii , esterase and glutathione S ‐transferase activity is commonly involved in resistance to organophosphates 23, 25, 41, 51, 52. In the present study, esterases could be involved in resistance to organophosphates; however, the high level of resistance to dimethoate (and hypersensitivity to carbofuran) is more likely related to the altered target site 21, 29, 50, 53…”

Section: Discussionmentioning

confidence: 47%

Insecticide resistance traits differ among and within host races in Aphis gossypii

Carletto¹,

Martin

Vanlerberghe‐Masutti³

et al. 2009

Pest Management Science

103

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Section: Discussionmentioning

confidence: 47%

Insecticide resistance traits differ among and within host races in Aphis gossypii

Carletto¹,

Martin

Vanlerberghe‐Masutti³

et al. 2009

Pest Management Science

103

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show abstract

“…A GST activity test was performed following a slightly modified version of a previously reported method . The enzyme source was prepared with 10 apterous adults in 1 mL of pre‐chilled PBS (0.1 mol L −1 , pH 7.6, containing 1.0 × 10 ‐3 mol L −1 EDTA) on ice and centrifuged at 4°C and 12 000 g for 10 min.…”

Section: Experimental Methodsmentioning

confidence: 99%

Characterisation of imidacloprid resistance in the bird cherry‐oat aphid, Rhopalosiphum padi, a serious pest on wheat crops

Wang

Zhang

Huang

et al. 2018

Pest Management Science

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“…Presently, the control of S. avenae and R. padi is primarily dependent on the application of insecticides. But the resistance of both aphid species developed slowly so few sprays are currently needed for the control of both S. avenae and R. padi (Chen et al 2007). Organophosphate and carbamate insecticides that are antiacetylcholinesterase (AChE) agents are widely used for insect control.…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on Acetylcholinesterase Characteristics between the Aphids,Sitobion avenaeandRhopalosiphum padi

Gao

2013

Journal of Insect Science

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The aphids Sitobion avenae (Fabricius) and Rhopalosiphum padi (Linnaeus) (Hemiptera: Aphidiae) are serious pests on grain crops and usually coexist on late period of wheat growth in China. Bioassays showed that R. padi was more susceptible than S. avenae to pirimicarb that is used for wheat aphid control, and the determination of acetylcholinesterase (AChE, EC 3.1.1.7) sensitivity showed that the sensitivity of AChE to pirimicarb was significantly higher in R. padi than in S. avenae (Lu and Gao 2009). AChE is the target enzyme of the carbamates, including pirimicarb, hence, to understand the mechanism responsible for the tolerance difference to carbamate insecticides of S. avenae and R. padi, we purified AChE from both aphid species using procainamide affinity column and characterized the AChE. The purification factor and yield from S. avenae (234.7-fold and 92.9%) were far higher than that from R. padi 17.3-fold and 13.9%. The results of substrate and inhibitor specificities of purified enzyme from both S. avenae and R. padi indicated that the purified enzyme was a typical AChE. The crude AChE extract from S. avenae was 5.4-, 4.3- and 8.1-fold less sensitive to inhibition by pirimicarb, methomyl and thiodicarb, respectively, than that from R. padi, whereas for the purified AChE, S. avenae was only 1.6-, 1.3- and 1.7-fold less sensitive to inhibition by pirimicarb, methomyl and thiodicarb, respectively, than R. padi. This suggests that eserine and BW284C51 may bind with other proteins, such as carboxylesterase, in the crude extract to reduce their inhibition against AChE. These results are useful for planning the chemical control of aphids on wheat.

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Resistance mechanisms and associated mutations in acetylcholinesterase genes in Sitobion avenae (Fabricius)

Cited by 44 publications

References 18 publications

Insecticide resistance traits differ among and within host races in Aphis gossypii

Insecticide resistance traits differ among and within host races in Aphis gossypii

Characterisation of imidacloprid resistance in the bird cherry‐oat aphid, Rhopalosiphum padi, a serious pest on wheat crops

Comparative Studies on Acetylcholinesterase Characteristics between the Aphids,Sitobion avenaeandRhopalosiphum padi

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