Insecticide resistance traits differ among and within host races in Aphis gossypii

Carletto, Jérôme; Martin, Thibaud; Vanlerberghe‐Masutti, Flavie; Brévault, Thierry

doi:10.1002/ps.1874

Cited by 101 publications

(81 citation statements)

References 52 publications

(75 reference statements)

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“…However, there were no marked changes in the LC 50 values of two susceptible A. gossypii strains to dimethoate when it was reared away from insecticide exposure in the laboratory. These results suggested that the parthenogenetic mode of reproduction in A. gossypii allowed rapid changes in resistance development to dimethoate over time [15]. The resistance development trend is in accordance with those studied in A. gossypii selected for imidacloprid in the laboratory [47] [48].…”

Section: Discussionsupporting

confidence: 84%

“…Detoxification is conferred by esterases (CarE and AChE), GSTs and MFOs. These enzymes are very important in many OP-resistant insect pests [15] [52] [53]. In this study, biochemical results revealed that an additive interaction of increased CarE, GSTs and PNOD activities in A. gossypii is correlated with resistance to dimethoate indicating metabolic resistance is the one of the means of dimethoate detoxification in the resistant strains.…”

Section: Discussionmentioning

confidence: 64%

“…In A. gossypii, resistance to OPs is due to enhanced metabolic destruction by detoxifying enzymes viz., carboxylesterases (CarE), glutathione S-transferases (GSTs), cytochrome P450-mediated p-Nitroanisole O-demethylase (PNOD) [4] [14] [15] and altered acetylcholinesterase (AChE) carrying point mutations [5] [16]. Generally, CarE combines with OPs (sequester) and hydrolyses insecticide esters forming non-toxic products and thereby prevents access of OPs to the target site, AChE [17].…”

Section: Introductionmentioning

confidence: 99%

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Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, Aphis gossypii(Hemiptera: Aphididae)

Lokeshwari¹,

Kumar²,

Manjunatha³

et al. 2016

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The melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a highly polyphagous sap sucking pest on wide varieties of crops including cotton and vegetables. It is a notorious vector of many plant viruses that are persistently and non-persistently transmitted. In nature, aphids are regulated by their natural enemies. However, chemical control remains a major management tool even though resistance to insecticides has been documented worldwide. A better understanding of mechanisms by which insecticide resistance occurs and its early detection is desirable to develop effective management strategies. The present investigation was conducted to study the development of resistance to an organophosphate (OP) compound-dimethoate, identify biochemical mechanism(s) involved in resistance and study cross-resistance to imidacloprid in laboratory selected A. gossypii strains in comparison to susceptible strains. Bioassay studies revealed that the LC50 values increased dramatically with dimethoate selection in resistant strains and the resistance ratio (RR) was 270-, 243-and 210-fold greater than that of the susceptible strains by 30 th generation. Further, biochemical assays revealed enhanced activities of carboxylesterases (CarE), glutathione S-transferases (GSTs) and cytochrome P450-mediated p-Nitroanisole O-demethylase (PNOD) in resistant strains supporting their role in dimethoate detoxification. This study thus revealed that enhanced activity of detoxifying enzymes viz., CarE, GSTs and PNODs is one of the mechanisms underlying dimethoate resistance in A. gossypii collected from South India. Interestingly, the possibility of negatively correlated cross-resistance to imidacloprid was identified in three OPresistant strains exhibiting 2.97-, 2.56-and 3.76-fold sensitivity to imidacloprid (a novel neonicotinoid). This indicated that the latter was less affected by the resistance mechanism(s) present.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

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Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, Aphis gossypii(Hemiptera: Aphididae)

Lokeshwari¹,

Kumar²,

Manjunatha³

et al. 2016

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“…22) e super-kdr mutation Met918 to r has previously been reported in A. gossypii. 23) Here we have provided direct evidence of an independent mechanism conferring pyrethroid resistance in A. gossypii in the form of kdr.…”

Section: Discussionmentioning

confidence: 69%

Detection of kdr pyrethroid resistance in the cotton aphid, Aphis gossypii (Hemiptera: Aphididae), using a PCR-RFLP assay

Marshall

Moran

Chen

et al. 2012

Journal of Pesticide Science

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Two point mutations, Leu1014 to Phe and Met918 to r, located in the domain II region of the para-type voltage gated sodium channel, confer knockdown resistance (kdr) to pyrethroid insecticides in several insect species. PCR ampli cation of this region in a pyrethroid-resistant and pyrethroid-susceptible strain of Aphis gossypii and use of a restriction fragment length polymorphism assay on PCR products (PCR-RFLP) provide the rst direct evidence of the Leu1014 to Phe kdr mutation in a eld isolate of A. gossypii.

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“…These active ingredients should be rotated to avoid the development of resistance, which was previously documented in citrus infesting aphids (Takada and Murakami, 1968;SeungSong et al, 1995;Nauen and Elbert, 2003;Li and Han, 2004;Toda et al, 2004;Carletto et al, 2010).…”

Section: Chemical Controlmentioning

confidence: 97%

Integrated Pest Management of Aphis spiraecola (Hemiptera: Aphididae) in clementines: enhancing its biological control

Marco¹

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A ma mare "la entrada del neolítico es la del abuso y la del sojuzgamiento, y en ella seguimos, inadaptados" Félix Rodríguez de la FuenteAgraïments Agraixc als meus directors Alberto Urbaneja i Alejandro Tena tota la feina que han fet i que m'han empentat a fer per aconseguir este primer escaló.Gracies Alberto per la teua confiança i dedicació des de abans del primer dia de doctorat, després de cinc anys i una tesi, ara ja podem ser amics.Gracies Alejandro per estar pendent cada dia, amb eixa dedicació e il•lusió.He disfrutat molt dels nostres debats científics. Ara ja soc mig enginyer?També he de agrair a Josep Jaques la seua col•laboració en gran part de la tesi. Gracies Josep per aportar de la teua clarividència. Es molt fàcil aprendre amb tres mostres com estos.Vull agrair infinitament a Pablo Bru tot el temps dedicat i tota la faena que hem compartit al camp. Sense la seua ajuda gran part de la tesis no haguera sigut possible. I per suposat a tots aquells que també han compartit hores de sol i calor, fang, pluja i fred o inclús rossejades de aplicacions quimiques. Vull agrair de una manera especial a dos persones que foren molt importants en dos moments claus de la meua carrera com Entomòleg. Sense la seua espenta en eixe instant, no estaria ací. Gracies Manel Porcar i Paco Beitia.I també a totes aquelles persones que durant mes de deu anys en convertiren de Biòleg a Entomòleg. Gracies a Lara Navarro, Teresa Oltra, Olaf Strauss, Axel Gruppe, Rosa Vercher i Ferran Garcia Marí, Tatiana Pina, Alfonso Hermoso de Mendoza, Maria Jesus Verdú, Jose Manuel Michelena, Paul Rugman-Jones i un llarg etc.Agrair a tots els meus amics i a totes aquelles persones especials que han compartit en mi el temps mentre esta tesi prenia forma. Sense ells, haguera sigut un viatge mes pesat.Especialment a Marta, en tu tot es molt més fàcil, ara et toca a tu.Nomes hem falta agrair a ma mare i mon pare tot el que han fet per a que el meu somni continués endavant, i sobre tot per els valors que m'ensenyaren de humilitat i perseverança. ResumAphis spiraecola Patch. (Hemiptera: Aphididae) és una de les plagues clau en el cultiu de clementins de la conca Mediterrània. En primavera, este àfid colonitza les brotacions tendres dels clementins i provoca importants pèrdues econòmiques tots els anys. Actualment la gestió integrada d"A. spiraecola en clementins està basada en el control químic ja que es desconeix prou sobre el control biològic d"A. spiraecola en cítrics i els esforços realitzats fins ara s"han centrat en el us i conservació de parasitoids encara que es desconeix les causes de la seua baixa eficàcia. Per altra banda, es coneix molt bé el conjunt de depredadors d"A. spiraecola però el seu impacte sobre les poblacions de l"àfid no s"han documentat. Tenint en compte estos antecedents, els objectius d"esta tesis foren: i) desentrampar les raons per les quals els parasitoids no són efectius; ii) determinar quan i com els depredadors poden controlar les poblacions d"A. spiraecola; i finalment iii) determinar si una coberta de poàcies po...

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Insecticide resistance traits differ among and within host races in Aphis gossypii

Abstract: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.

Cited by 101 publications

References 52 publications

Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, <i>Aphis gossypii</i>(Hemiptera: Aphididae)

Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, <i>Aphis gossypii</i>(Hemiptera: Aphididae)

Detection of <i>kdr</i> pyrethroid resistance in the cotton aphid, <i>Aphis gossypii</i> (Hemiptera: Aphididae), using a PCR-RFLP assay

Integrated Pest Management of Aphis spiraecola (Hemiptera: Aphididae) in clementines: enhancing its biological control

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Insecticide resistance traits differ among and within host races in Aphis gossypii

Abstract: Multiple resistance to a broad range of insecticides and multiple mechanisms of resistance in some clones could explain to some extent the low genetic diversity observed within A. gossypii host races.

Cited by 101 publications

References 52 publications

Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, &lt;i&gt;Aphis gossypii&lt;/i&gt;(Hemiptera: Aphididae)

Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, &lt;i&gt;Aphis gossypii&lt;/i&gt;(Hemiptera: Aphididae)

Detection of <i>kdr</i> pyrethroid resistance in the cotton aphid, <i>Aphis gossypii</i> (Hemiptera: Aphididae), using a PCR-RFLP assay

Integrated Pest Management of Aphis spiraecola (Hemiptera: Aphididae) in clementines: enhancing its biological control

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Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, <i>Aphis gossypii</i>(Hemiptera: Aphididae)

Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, <i>Aphis gossypii</i>(Hemiptera: Aphididae)