Characterisation of imidacloprid resistance in the bird cherry‐oat aphid, <i>Rhopalosiphum padi,</i> a serious pest on wheat crops

Wang, Kang; Zhang, Meng; Huang, Yanna; Yang, Zhuolin; Su, Shiliang; Chen, Maohua

doi:10.1002/ps.4834

Cited by 67 publications

(66 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytochrome P450 enzyme levels were measured using the heme peroxidation method (Wang et al, 2018;William and Janet, 1997). Briefly, the total reaction volume was 650 μl, consisting of enzyme sample (40 μl), potassium phosphate buffer (160 μl, 0.625 M, pH 7.0), 50 μl H 2 O 2 (3%) and 400 μl of 3,3',5,5'-tetra-methylbenzidine (TMBZ) solution (Sigma…”

Section: Enzyme Activity Assaysmentioning

confidence: 99%

Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi

Rameshgar

Khajehali

Nauen

et al. 2019

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

show abstract

Section: Enzyme Activity Assaysmentioning

confidence: 99%

Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi

Rameshgar

Khajehali

Nauen

et al. 2019

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

show abstract

“…The control of this aphid has depended largely on the use of chemically synthesized pesticides, 20 such as imidacloprid, which is a neonicotinoid that targets sucking pests, such as aphids and the green leaf bug. 21 It has been widely applied in wheat fields to control wheat aphid in China. As a result, S. avenae has developed serious resistance to imidacloprid.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed microRNAs under imidacloprid exposure in Sitobion avenae Fabricius

Zhang

Xie

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: MicroRNAs (miRNAs), which are short single-stranded non-coding RNAs, regulate the expression of target genes, especially those involved in the regulation or metabolism of endogenous or xenobiotic compounds. Results: De novo assemblies of the transcriptome of Sitobion avenae Fabricius under control conditions and under imidacloprid treatment were obtained using Illumina short-read sequencing technology. Fifty-seven miRNAs, of which 36 were known and 21 were novel, were identified. Quantitative analysis of miRNA levels showed that 5 miRNAs were significantly up-regulated, and 11 miRNAs were significantly down-regulated in the nymphs of S. avenae treated with imidacloprid in comparison with those of the control. Analysis of the candidate target genes in S. avenae that could be regulated by these miRNAs were also carried out. The functions of the miRNAs, which could potentially regulate target genes that participate in metabolism, regulatory or detoxification pathways in S. avenae, were clarified based on Gene Ontology and KEGG pathway analysis. The effects of the miRNAs api-miR-1000, api-miR-316, and api-miR-iab-4 on susceptibility of S. avenae to imidacloprid was determined. Modulation of the abundances of api-miR-1000, api-miR-316, and api-miR-iab-4 by the addition of the correspondign inhibitors to the artificial diet significantly changed the susceptibility of S. avenae to imidacloprid, which further demonstrated the effect of these miRNAs on the regulation or metabolism of insecticides.Conclusion: The results of this study suggested that miRNAs differentially expressed in response to imidacloprid could play a critical regulatory role in the resistance of S. avenae to imidacloprid.

show abstract

“…Imidacloprid, a hloronicotinyl insecticide that targets aphids, leafhoppers, whiteflies and other sucking pests, has been widely used for the control of wheat aphid in China. Consequently, wheat aphid has evolved serious resistance to imidacloprid . Previous studies have demonstrated that metabolic and target‐site mechanisms are associated with imidacloprid resistance in many insect pests .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, wheat aphid has evolved serious resistance to imidacloprid. [6][7][8][9] Previous studies have demonstrated that metabolic and target-site mechanisms are associated with imidacloprid resistance in many insect pests. 10,11 For example, the R81T mutation of the nAChR 1 subunit confers resistance to imidacloprid in cotton aphid 5,[12][13][14] and overexpression of P450 genes is associated with imidacloprid resistance in Nilaparvata lugens and Leptinotarsa decemlineata.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA interference

Liu

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Insecticides act as toxins, inhibitors of digestion and deterrents, and affect the expression of many genes in insects. To assess key genes associated with the detoxification or regulation of imidacloprid in greenbug, Schizaphis graminum (Rondani), the transcriptome and digital gene expression (DGE) profile were analyzed using Illumina sequencing. RESULTS In total, 48 763 494 clean reads were obtained by sequencing. Expression profile analysis showed that 2782 unigenes were differently expressed between the imidacloprid treatment and control groups. After exposure to imidacloprid, the expression levels of 1846 unigenes were upregulated and 936 were downregulated in comparison with controls. Expression patterns of the top 20 highly expressed genes show that they could be involved in the detoxification of imidacloprid. Silencing of multidrug resistance‐associated gene (MRA), GATA‐binding gene (GAT) and takeout‐like precursor gene (TLP) resulted in increasing susceptibility to imidacloprid. CONCLUSIONS The differentially expressed genes in S. graminum have potential regulatory or detoxification roles in response to imidacloprid. These results should be useful in understanding the molecular mechanisms of greenbug adaption to imidacloprid. © 2018 Society of Chemical Industry

show abstract

Characterisation of imidacloprid resistance in the bird cherry‐oat aphid, Rhopalosiphum padi, a serious pest on wheat crops

Abstract: Multiple mechanisms confer imidacloprid resistance in R. padi. © 2017 Society of Chemical Industry.

Cited by 67 publications

References 60 publications

Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi

Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi

Identification of differentially expressed microRNAs under imidacloprid exposure in Sitobion avenae Fabricius

Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA interference

Contact Info

Product

Resources

About