Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi

Rameshgar, Fariborz; Khajehali, Jahangir; Nauen, Ralf; Bajda, Sabina; Jonckheere, Wim; Dermauw, Wannes; Leeuwen, Thomas Van

doi:10.1016/j.pestbp.2019.03.008

Cited by 18 publications

(13 citation statements)

References 42 publications

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“…For the Tetranychus species, several mutations in the VGSC have also been associated with decreased sensitivity towards Type I and Type II pyrethroids: point mutations M918L/T or L925M in the intracellular linker connecting transmembrane segments IIS4 and IIS5 (where M918L was found in combination with F1534S in IIIS6), L1024V at the terminus of the IIS6 transmembrane segment of the sodium channel and F1538I in domain IIIS6 ( M. domestica numbering) [ 16 , 17 , 18 , 19 , 20 , 21 ] ( Figure 1 ) ( Table S1 ). Similar VGSC mutations have also been reported for other spider mites such as Panonychus citri and Panonychus ulmi , but also in other mites and ticks, including the cattle tick Rhipicephalus microplus , the Varroa mite, the poultry red mite Dermanyssus gallinae and predatory mites such as Phytoseiulus persimilis [ 22 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Introductionsupporting

confidence: 69%

“…To examine whether the difference in susceptibility to bifenthrin could be explained by target-site resistance mutations, we screened for known mutations in the VGSC and found that three out of four bifenthrin-resistant strains carried the L1024V mutation in the VGSC (MR-VL, MR-VLi and ES1). Next to the L1024V mutation, the most resistant strain in this study, ES1, also showed the presence of the F1538I mutation, which has been frequently associated with pyrethroid resistance [ 16 , 17 , 18 , 19 , 30 , 91 , 92 , 93 , 94 ]. We did find the F1538I mutation in the VGSC of the susceptible strain UK4, but it was at a low frequency and therefore probably did not result in resistance in our bioassays.…”

Section: Discussionmentioning

confidence: 93%

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High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

Beer

Vandenhole

Njiru

et al. 2022

Biology

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Pyrethroids are widely applied insecticides in agriculture, but their frequent use has provoked many cases of resistance, in which mutations in the voltage-gated sodium channel (VGSC), the pyrethroid target-site, were shown to play a major role. However, for the spider mite Tetranychus urticae, it has also been shown that increased detoxification contributes to resistance against the pyrethroid bifenthrin. Here, we performed QTL-mapping to identify the genomic loci underlying bifenthrin resistance in T. urticae. Two loci on chromosome 1 were identified, with the VGSC gene being located near the second QTL and harboring the well-known L1024V mutation. In addition, the presence of an L925M mutation in the VGSC of a highly bifenthrin-resistant strain and its loss in its derived, susceptible, inbred line indicated the importance of target-site mutations in bifenthrin resistance. Further, RNAseq experiments revealed that genes encoding detoxification enzymes, including carboxyl/choline esterases (CCEs), cytochrome P450 monooxygenases and UDP-glycosyl transferases (UGTs), were overexpressed in resistant strains. Toxicity bioassays with bifenthrin (ester pyrethroid) and etofenprox (non-ester pyrethroid) also indicated a possible role for CCEs in bifenthrin resistance. A selection of CCEs and UGTs were therefore functionally expressed, and CCEinc18 was shown to metabolize bifenthrin, while teturUGT10 could glycosylate bifenthrin-alcohol. To conclude, our findings suggest that both target-site and metabolic mechanisms underlie bifenthrin resistance in T. urticae, and these might synergize high levels of resistance.

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Section: Introductionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 93%

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

Beer

Vandenhole

Njiru

et al. 2022

Biology

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“…Tabela 2. Studije koje potvrđuju pojavu rezistentnosti kod P. ulmi na određene aktivne materije koje su danas u upotrebi prema Badieinia et al (2020) Aktivna materija Literaturni izvor abamektin* Nauen et al, 2001;Çağatay et al, 2014;Rameshgar et al, 2019bdikofol Pree et al, 2005fenazakvin Auger et al, 2003fenpiroksimat Nauen et al, 2001Yaghoobi et al, 2021heksitiazoks* Thwaite, 1991Nauen et al, 2001;Pree et al, 2005klofentezin* Thwaite, 1991Nauen et al, 2001;Pree et al, 2005;Kramer andNauen, 2011 piretroidi Nauen et al, 2001;Rameshgar et al, 2019apiridaben* Nauen et al, 2001Kramer andNauen, 2011 spirodiklofen* Kramer andNauen, 2011;Bajda et al, 2015tebufenpirad* Auger et al, 2003 aktivne materije koje su registrovane u Srbiji za suzbijanje P. ulmi na jabuci prema (Grupa autora, 2020) Primera radi, spirodiklofen i spiromesifen su dva ciklična keto-enolna jedinjenja koja pokazuju veoma dobro akaricidno delovanje na fi tofagne grinje i nekoliko štetnih vrsta insekata (Dekeyser, 2005). Prema Bretschneider et al (2012) i Bajda et al (2015, ova jedinjenja inhibiraju acetil-CoA karboksilazu (ACCase) i ometaju biosintezu lipida kod grinja i insekata.…”

Section: Mehanizmi Rezistentnosti I Načini Delovanja Akaricida Kod P Ulmiunclassified

Factors and mechanisms of acaricide resistance in European red mite (Panonychus ulmi Koch)

Petrović¹,

Ivanović²,

Manojlović³

et al. 2021

Biljni lekar

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Inadequate choice and application of control measures for agricultural pests, ectoparasites of livestock and domestic animals, vectors and reservoirs of various diseases of human medicine and veterinary importance in urban areas has caused the resistance occurrence and cross-resistance in a large number of arthropods. Their control is becoming increasingly difficult and challenging. For example, the use of acaricides is indispensable in the control of European red mite (Panonychus ulmi, Acari: Tetranychide), but also for other species from this family that are all significant pests in agricultural production, horticulture and forestry. The resistance occurrence and maintenance and cross-resistance in arthropods is a global phenomenon. The aim of this paper was to present the resistance factors and mechanisms in Panonychus ulmi control and to list the active substances for which there was an evidence that resistance has been acquired, all with the goal of pointing out the problem of resistance in this species and highlight the ways to prevent it. The occurrence and evolution of resistant arthropod populations could have multiple consequences that can be observed from an agricultural, economic, health, and environmental perspective.

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“…7 Many mutations which are in different position of insect sodium channel genes were reported in insect field populations. [8][9][10][11] Some mutations have been proved to reduce the combination between insect sodium channels with pyrethroid in Xenopus oocytes. 12,13 Some mutations are identified in multiple insect species, but some mutations are found only in single species.…”

Section: Introductionmentioning

confidence: 99%

“…A major molecular mechanism of insect resistance to pyrethroids is known as knockdown (kdr) 7 . Many mutations which are in different position of insect sodium channel genes were reported in insect field populations 8–11 . Some mutations have been proved to reduce the combination between insect sodium channels with pyrethroid in Xenopus oocytes 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Two classic mutations in the linker‐helix IIL45 and segment IIS6 of Apolygus lucorum sodium channel confer pyrethroid resistance

Wang

Lin

Wang

et al. 2020

Pest Management Science

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BACKGROUND: Pyrethroids are classified as type I and type II for distinct symptomology. Voltage-gated sodium channel is a primary target of pyrethroids. Mutations of the insect sodium channel have been identified to result in resistance to pyrethroids. Double mutation (L 1002 F/M 906 I) was detected in field-strain of Apolygus lucorum (Meyer-Dür). Although, it was illuminated the function of the same position mutation in other pests, it is necessary to demonstrate the role in A. lucorum. RESULTS: In this study, we examined the effects of mutations on channel gating and pyrethroid sensitivity in Xenopus oocytes. L 1002 F, M 906 I and L 1002 F/M 906 I all shifted the voltage dependence of activation in the depolarizing direction. L 1002 F, M 906 I and L 1002 F/M 906 I all reduced the amplitude of tail currents induced by type I (bifenthrin and permethrin) and type II (⊗-cyhalothrin and deltamethrin). The double mutation, L 1002 F/M 906 I, reduced integral channel modification by 10-fold compared with the L 1002 F and M 906 I mutations alone, respectively. Computational analysis based on the model of dual pyrethroid receptors, the two resistance mutations, L 1002 F and M 906 I are facing two opposite sides of this newly identified pocket. Both mutations affect the optimal binding of the ligands by changing the shape of the pocket but in different ways. CONCLUSION: Our results illustrate the distinct effect of mutations on pyrethroids. It is predicted with computer modeling that these mutations allosterically affect pyrethroid binding.

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Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi

Abstract: Point mutations in the voltage-gated sodium channel gene associated with pyrethroid resistance in Iranian populations of the European red mite Panonychus ulmi.

Cited by 18 publications

References 42 publications

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

High-Resolution Genetic Mapping Combined with Transcriptome Profiling Reveals That Both Target-Site Resistance and Increased Detoxification Confer Resistance to the Pyrethroid Bifenthrin in the Spider Mite Tetranychus urticae

Factors and mechanisms of acaricide resistance in European red mite (Panonychus ulmi Koch)

Two classic mutations in the linker‐helix IIL45 and segment IIS6 of Apolygus lucorum sodium channel confer pyrethroid resistance

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