Research progress on Traditional Chinese Medicine syndromes of diabetes mellitus

Wang, Jingkang; Ma, Quantao; Li, Yaqi; Li, Pengfei; Wang, Min; Wang, Tieshan; Wang, Chunguo; Wang, Ting; Zhao, Baosheng

doi:10.1016/j.biopha.2019.109565

Cited by 80 publications

(46 citation statements)

References 56 publications

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“…After thousands of years of development, numerous herbal formulae of Traditional Chinese Medicine (TCM) have been widely applied in multiple diseases, including cancer, 8 diabetes mellitus, 9 and so on. Besides, over the last few decades, TCM has been playing a more and more significant role in Chinese clinical practice especially during the COVID-19 pandemic in 2019.…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology Prediction and Molecular Docking-Based Strategy to Discover the Potential Pharmacological Mechanism of Huai Hua San Against Ulcerative Colitis

Liu¹,

Liu²,

Tong³

et al. 2021

DDDT

109

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Background: Huai Hua San (HHS), a famous Traditional Chinese Medicine (TCM) formula, has been widely applied in treating ulcerative colitis (UC). However, the interaction of bioactives from HHS with the targets involved in UC has not been elucidated yet. Aim: A network pharmacology-based approach combined with molecular docking and in vitro validation was performed to determine the bioactives, key targets, and potential pharmacological mechanism of HHS against UC. Materials and Methods: Bioactives and potential targets of HHS, as well as UC-related targets, were retrieved from public databases. Crucial bioactive ingredients, potential targets, and signaling pathways were acquired through bioinformatics analysis, including protein-protein interaction (PPI), as well as the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Subsequently, molecular docking was carried out to predict the combination of active compounds with core targets. Lastly, in vitro experiments were conducted to further verify the findings. Results: A total of 28 bioactive ingredients of HHS and 421 HHS-UC-related targets were screened. Bioinformatics analysis revealed that quercetin, luteolin, and nobiletin may be potential candidate agents. JUN, TP53, and ESR1 could become potential therapeutic targets. PI3K-AKT signaling pathway might play an important role in HHS against UC. Moreover, molecular docking suggested that quercetin, luteolin, and nobiletin combined well with JUN, TP53, and ESR1, respectively. Cell experiments showed that the most important ingredient of HHS, quercetin, could inhibit the levels of inflammatory factors and phosphorylated c-Jun, as well as PI3K-Akt signaling pathway in LPS-induced RAW264.7 cells, which further confirmed the prediction by network pharmacology strategy and molecular docking. Conclusion:Our results comprehensively illustrated the bioactives, potential targets, and molecular mechanism of HHS against UC. It also provided a promising strategy to uncover the scientific basis and therapeutic mechanism of TCM formulae in treating diseases.

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Section: Introductionmentioning

confidence: 99%

Network Pharmacology Prediction and Molecular Docking-Based Strategy to Discover the Potential Pharmacological Mechanism of Huai Hua San Against Ulcerative Colitis

Liu¹,

Liu²,

Tong³

et al. 2021

DDDT

109

View full text Add to dashboard Cite

show abstract

“…The relationship between the change of intestinal microbiota environment and the occurrence of metabolic diseases such as obesity and diabetes is moving from correlation to causation [16]. Intestinal microbiota disorder, such as the decrease of beneficial bacteria and the increase of harmful bacteria, is easy to occur in prediabetes and type 2 diabetes (T2D) patients [17]. Specific changes in intestinal microbiota are considered to be one of the causes of metabolic diseases [16,18].…”

Section: Introductionmentioning

confidence: 99%

Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats

Cai

Zhou

Song

et al. 2020

BioMed Research International

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Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes. Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats. Rats were given 50 mg/kg 3DG by intragastric administration for two weeks. Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence. The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively. 3DG treatment did not significantly change the richness and evenness but affected the composition of intestinal microbiota. At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria. Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group. The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group. In addition, 45 KEGG pathways were altered after two-week intragastric administration of 3DG. Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides. Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance. The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.

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“…Recently, Traditional Chinese Medicine (TCM) has achieved good efficacy in the treatment of DN. Numerous TCMs have been used in clinical practice and have shown promising results in the treatment of diabetes and its complications (31,32). Unlike a single compound that has only one active chemical component, TCM is usually a complex combination of chemicals that work synergistically.…”

Section: Discussionmentioning

confidence: 99%

Dendrobium mixture attenuates renal damage in rats with diabetic nephropathy by inhibiting the PI3K/Akt/mTOR pathway

et al. 2021

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notable glomerular hypertrophy, an increase in mesangial matrix content and renal interstitial fibrosis. Moreover, DMix notably reduced kidney damage. The results demonstrated that DMix inhibited the phosphorylation of PI3K, Akt and mTOR in the kidney tissues of rats with DN, and increased the protein and mRNA expression levels of LC3 and Beclin-1. Therefore, it was suggested that DMix has protective effects on the kidney of rats with DN, which may be associated with the inhibition of the PI3K/Akt/mTOR signaling pathway and activation of renal autophagy by this traditional medicine.

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Research progress on Traditional Chinese Medicine syndromes of diabetes mellitus

Cited by 80 publications

References 56 publications

Network Pharmacology Prediction and Molecular Docking-Based Strategy to Discover the Potential Pharmacological Mechanism of Huai Hua San Against Ulcerative Colitis

Network Pharmacology Prediction and Molecular Docking-Based Strategy to Discover the Potential Pharmacological Mechanism of Huai Hua San Against Ulcerative Colitis

Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats

Dendrobium mixture attenuates renal damage in rats with diabetic nephropathy by inhibiting the PI3K/Akt/mTOR pathway

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