Research on Magnetic Separation Methods for the Extraction of Nucleic Acids

Abstract

The magnetic separation system is an important part of the automatic testing equipment. There are two methods to magnetize, internal magnetic attracting and external magnetic attracting. We compare the difference of the realization methods and the magnetic field distribution of the two ways. Then we select appropriate method to magnetize.

Effects of different quantities of antibody conjugated with magnetic nanoparticles on cell separation efficiency

“…MNPs have shown promise in biotechnology due to their low toxicity, superparamagnetism, high surface area and simple separation [9,10,11,12]. While, the high viscosity and large sizes of microbeads prevented their efficient interaction with cell surfaces, the higher surface area rather than microparticles and an efficient nano sizes of nanoparticles facilitate the bioseparation and purification of a variety of biomacromolecules and cells [13] including proteins [14], nucleic acids [15], viruses [16], bacteria [17,18], and cancerous cells [19]. They have also been applied for drug delivery [20,21], magnetic resonance imaging [22,23,24,25], hyperthermia [26], diagnosis and treatment in various cancers [27,28,29,30], and magnetic separation [31,32].…”

Applications of magnetic materials separation in biological nanomedicine

“…In this case, MNPs have no attraction for each other, thereby reducing the risk of particle aggregation . In summary, the above mentioned characteristics provide distinct features for their wide use at the time being in various applications belonging to the field of biomedical separation and purification, for instance, they are used as carriers for cell separation , nucleic acids isolation , protein purification , enzyme immobilization , blood detoxification , and rare cancer cell detection .…”