Research on Hypnotic and Anticonvulsant Activities of Total Alkaloids in Leaves of Eucommia ulmoides

Li, Xin; Yang, Ling; Wang, Liping; Shao-yang, Liu; Liu, Yan

doi:10.1016/s1674-6384(14)60020-4

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…E. ulmoides is also occasionally planted in botanical gardens in Europe, North America and elsewhere. It shows various pharmacological properties, including anti-oxidative [1], protection against cytotoxicity [2], benefits the liver and kidney [3], lowers blood hypertension [4], hypoglycemic [5], hypnotic and anticonvulsant activities [6], neuroprotective effects [7], weight loss [8], strengthening tendons and bones [9], and other pharmacological effects [10]. The bark of E. ulmoides is one of the oldest tonic traditional Chinese medicines and officially listed in the Chinese Pharmacopoeia.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment

Guo

Zhao

et al. 2015

Molecules

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A continuous process based on the combination of ultrasounds and/or microwaves pretreatments followed by enzymatic hydrolysis and simultaneous extraction (EHSE) has been proposed to recover genipin from Eucommia ulmoides bark. At first, in the pretreatment step, the mixture of 1.0 g dried bark powder and 10 mL deionized water were irradiated by microwave under 500 W for 10 min. Then, in hydrolysis step, the optimal conditions were as follows: 0.5 mg/mL of cellulase concentration, 4.0 pH of enzyme solution, 24 h of incubation time and 40 °C of incubation temperature. After incubation, 10 mL ethanol was added to extract genipin for 30 min by ultrasound. After EHSE treatment, the yield of genipin could reach 1.71 μmol/g. Moreover, scanning electron micrographs illustrated that severe structural disruption of plant was obtained by EHSE. The results indicated that the EHSE method provided a good alternative for the preparation of genipin from Eucommia ulmoides bark as well as other herbs.

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