Online extraction–HPLC–FRAP system for direct identification of antioxidants from solid Du-zhong brick tea

Shi, Shuyun; Guo, Keke; Tong, Runna; Liu, Yuge; Tong, Chaoying; Peng, Mijun

doi:10.1016/j.foodchem.2019.03.013

Cited by 49 publications

(17 citation statements)

References 25 publications

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“…HPLC with diode array detector (DAD) and MS/MS has been developed for rapid isolation and characterization of bioactive components from complex natural products [8–11]. However, information about comprehensive analysis of homoisoflavonoids, especially for minors, in O. japonicus by HPLC–DAD–MS/MS is restricted because of the existence of multicomponents, restriction of detection sensitivity, and coelution of minor and abundant compounds [12,13]. Therefore, techniques to enrich minors and improve separation resolutions are necessary for profiling homoisoflavonoids in O. japonicus .…”

Section: Introductionmentioning

confidence: 99%

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Deng

Tong

et al. 2020

J of Separation Science

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Roots of Ophiopogon japonicus have been used as a functional food ingredient and traditional Chinese medicine for a long time in China. Homoisoflavonoids are one of the major kinds of bioactive compounds in O. japonicus; however, literature data about its homoisoflavonoids profile are scarce because of the complex ingredients with low abundance. Here, homoisoflavonoid fraction was prepared by petroleum ether extraction. Then, a high-speed countercurrent chromatography offline coupling with high-performance liquid chromatography-diode array detectorquadrupole time-of-flight tandem mass spectrometry was developed for systematic identification of homoisoflavonoids. After that, 39 homoisoflavonoids, including 29 homoisoflavanone and 10 homoisoflavone, were unambiguously or tentatively identified, while 12 of them were reported in O. japonicus for the first time. Finally, eight available homoisoflavonoids were sensitively, precisely, and accurately determined by standard calibration curves, with limit of detection and limit of quantification in the range of 0.05-0.30 μg/mL and 0.12-0.66 μg/mL, relative standard deviation less than 7.3% for intra-and interday variations, and recovery at 94.5-105.2%. Collectively, our developed method is efficient, reliable, and valuable to profile chemical components of complex natural products.

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Section: Introductionmentioning

confidence: 99%

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Deng

Tong

et al. 2020

J of Separation Science

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“…Eucommia ulmoides leaves (EL) are rather ubiquitous as the by-product of traditional Chinese medicinal herb Du-zhong. EL is rich in bioactive compounds (such as flavonoids, chlorogenic acid, aucubin, and geniposidic acid) and has anti-inflammatory, antioxidant, antiviral and hepatoprotective functions [8–12]. Therefore, EL is regarded to be a beneficial antioxidant feed additive.…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with the extract from Eucommia ulmoides leaves changed epithelial restitution and gut microbial community and composition of weanling piglets

Peng¹,

Wang²,

Peng³

et al. 2019

PLoS ONE

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This study was conducted to compare the effects of Eucommia ulmoides leaves (EL) in different forms (EL extract, fermented EL, and EL powder) with antibiotics on growth performance, intestinal morphology, and the microbiota composition and diversity of weanling piglets. Compared to the control group, the antibiotics and EL extract significantly increased the average daily gain and decreased the feed: gain ratio as well as the diarrhea rate (P < 0.05). The EL extract significantly decreased the crypt depth and increased the ratio of villus height to crypt depth (P < 0.05), while the fermented EL group did the opposite (P < 0.05). The crypt depth in the antibiotics group was of similar value to the EL extract group, and was lower than the fermented EL and EL powder groups (P < 0.05). Compared to the control and antibiotics groups, the jejunul claudin-3 mRNA expression and the concentrations of total VFA, Chao 1, and ACE were significantly augmented in the EL extract group of piglets (P < 0.05). The EL extract groups also showed elevated Shannon (P < 0.05) and Simpson (P = 0.07) values relative to the control and antibiotics groups. At the phylum level, the EL extract group exhibited a reduced abundance of Bacteroidetes and an enhanced abundance of Firmicutes. At the genus level, the abundance of Prevotella was augmented in the EL extract group. Moreover, compared with the antibiotic group, the acetate concentration was enhanced in the EL extract and fermented EL groups. Overall, dietary supplementation with the EL extract, but not the fermented EL or EL powder, improved growth performance, jejunul morphology and function, as well as changed colonic microbial composition and diversity, which might be an alternative to confer protection against weanling stress in weanling piglets.

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“…To conduct a detailed quantitative analysis of the standards in the two extracts, HPLC analysis was implemented upon the pure standards with different concentrations ranged from 10 mg L − 1 to 500 mg L − 1 for calibration [40] . As shown in Fig 2 , the retention time peak of these three standards increased with the increase in concentration of GA, EGCG and CAF.…”

Section: Resultsmentioning

confidence: 99%

Exploring community evolutionary characteristics of microbial populations with supplementation of Camellia green tea extracts in microbial fuel cells

Qin

Guo

et al. 2020

Journal of the Taiwan Institute of Chemical Engineers

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Online extraction–HPLC–FRAP system for direct identification of antioxidants from solid Du-zhong brick tea

Cited by 49 publications

References 25 publications

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Dietary supplementation with the extract from Eucommia ulmoides leaves changed epithelial restitution and gut microbial community and composition of weanling piglets

Exploring community evolutionary characteristics of microbial populations with supplementation of Camellia green tea extracts in microbial fuel cells

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