EuRBG10 involved in indole alkaloids biosynthesis in Eucommia ulmoides induced by drought and salt stresses

Zuo, Yunjuan; Li, Bairu; Guan, Suixia; Jia, Jingyu; Xu, Xinjie; Zhang, Zilong; Lu, Zheng; Li, Xin; Pang, Xinyue

doi:10.1016/j.jplph.2022.153813

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…SCINA 66 and known marker genes were employed for cell type identification. In this study, PCA 67 and UMAP 68 were used as dimensionality reduction algorithms.…”

Section: Methodsmentioning

confidence: 99%

Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence

Li,

et al. 2024

Nat Commun

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The senescence of fruit is a complex physiological process, with various cell types within the pericarp, making it highly challenging to elucidate their individual roles in fruit senescence. In this study, a single-cell expression atlas of the pericarp of pitaya (Hylocereus undatus) is constructed, revealing exocarp and mesocarp cells undergoing the most significant changes during the fruit senescence process. Pseudotime analysis establishes cellular differentiation and gene expression trajectories during senescence. Early-stage oxidative stress imbalance is followed by the activation of resistance in exocarp cells, subsequently senescence-associated proteins accumulate in the mesocarp cells at late-stage senescence. The central role of the early response factor HuCMB1 is unveiled in the senescence regulatory network. This study provides a spatiotemporal perspective for a deeper understanding of the dynamic senescence process in plants.

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“…SCINA 66 and known marker genes were employed for cell type identification. In this study, PCA 67 and UMAP 68 were used as dimensionality reduction algorithms.…”

Section: Methodsmentioning

confidence: 99%

Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence

Li,

et al. 2024

Nat Commun

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“…Including the induction of plant osmoregulation, ion transport, antioxidants and other related genes [16,17,18]. Studies have found that the application of exogenous plant growth regulators under stress can improve plant resistance [19,20,21,22]. Melatonin, a hormone-like substance widely distributed in higher plants, plays an important role in alleviating abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Melatonin Strengthens Saline-alkali Stress Tolerance in M9-T337 Seedlings by Initiating a Variety of Physiological and Biochemical Pathways

Xian,

Zhang,

Wang

et al. 2024

Preprint

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Melatonin (MT) is an important phytohormone that significantly regulates the growth and development of plants. Previous studies confirmed the effectiveness of MT in improving plant stress tolerance. In this study, annual M9-T337 seedlings were selected as subjects and five treatments were applied: conventional control (CK), in which only half the concentration of Hoagland was applied; Saline-alkali stress treatment (SA, 100 mmol·L-1 Saline-alkali solution); melatonin treatment (MT, CK + 200 μmol·L-1 exogenous MT); Saline-alkali + melatonin treatment (MS, SA + 200 μmol·L-1 exogenous MT); and Saline-alkali stress + melatonin + inhibitor treatment (HS, additional 100 μmol·L-1 p-CPA treatment to MS). The results showed that Saline-alkali stress negatively affected the growth of M9-T337 seedlings by reducing photosynthetic capacity, increasing Na+, promoting reactive oxygen species such as H2O2, and changing the osmotic content and antioxidant system. However, the application of exogenous MT effectively alleviated Saline-alkali damage and significantly promoted the growth of M9-T337 seedlings. It significantly increased plant height, diameter, root length, root surface area, volume and activity. Furthermore, MT alleviated osmotic stress by accumulating proline, soluble sugars, soluble proteins and starch. Furthermore, MT improved photosynthetic capacity by delaying chlorophyll degradation and regulating gas exchange parameters as well as fluorescence parameters in leaves. Furthermore, MT improved the Na+/K+ ratio to reduce ion toxicity by upregulating the expression of Na+ transporter genes (MhCAX5, MhCHX15, MhSOS1, and MhALT1) and downregulating the expression of K+ transporter genes (MhSKOR and MhNHX4). In addition, MT can increase antioxidant enzyme activity (SOD, POD, CAT, AAO, APX and MDH) in the ASA-GSH cycle and increase AsA, GSH and GSSG levels to counteract the accumulation of reactive oxygen species (ROS). such as H2O2 and O2-, reducing oxidative damage. Exogenous MT promotes root growth under salt-alkaline stress by increasing root activity and responding synergistically with IAA, GA3 and ZT to salt-alkaline stress. Our results confirm that MT has the potential to alleviate Saline-alkali stress by promoting root growth, increasing biomass accumulation and photosynthetic capacity, strengthening the antioxidant defense system, maintaining ionic balance, the ascorbate-glutathione cycle and the Osmoregulation facilitates and regulates endogenous hormone levels in M9-T337 seedlings.

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“…Under the global climate change situation, the growth and development of trees are increasingly threatened by drought and salt stress ( Polle and Chen, 2015 ; Choat et al., 2018 ; McDowell et al., 2020 ; Yu et al., 2023 ). Recent researches on the response of E. ulmoides to salt and drought stress mainly focus on the morphology, physiology and molecular biology ( Wang et al., 2018 ; Li et al., 2019 ; Zuo et al., 2022 ), however, its response patterns of NSCs and its composition under stress is still not clear. As well as we known, no study has been conducted to compare the similarities and differences of its NSCs response patterns under both salt and drought stress simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Growth and non-structural carbohydrates response patterns of Eucommia ulmoides under salt and drought stress

Zhang,

Qin,

Kan

et al. 2024

Front. Plant Sci.

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IntroductionSalinity and droughts are severe abiotic stress factors that limit plant growth and development. However, the differences and similarities of non-structural carbohydrates (NSCs) responses patterns of trees under the two stress conditions remain unclear.MethodsWe determined and compared the growth, physiology, and NSCs response patterns and tested the relationships between growth and NSCs concentrations (or pool size) of Eucommia ulmoides seedlings planted in field under drought and salt stress with different intensities and durations.Results and discussionWe found that drought and salt stress can inhibit the growth of E. ulmoides, and E. ulmoides tended to enhance its stress resistance by increasing proline concentration and leaf thickness or density but decreasing investment in belowground biomass in short-term stress. During short-term drought and salt stress, the aboveground organs showed different NSCs response characteristics, while belowground organs showed similar change characteristics: the starch (ST) and NSCs concentrations in the coarse roots decreased, while the ST and soluble sugar (SS) concentrations in the fine roots increased to enhance stress resistance and maintain water absorption function. As salt and drought stress prolonged, the belowground organs represented different NSCs response patterns: the concentrations of ST and SS in fine roots decreased as salt stress prolonged; while ST in fine roots could still be converted into SS to maintain water absorption as drought prolonged, resulting in an increase of SS and a decrease of ST. Significant positive relationships were found between growth and the SS and total NSCs concentrations in leaves and branches, however, no significant correlations were found between growth and below-ground organs. Moreover, relationships between growth and NSCs pool size across organs could be contrast.ConclusionOur results provide important insights into the mechanisms of carbon balance and carbon starvation and the relationship between tree growth and carbon storage under stress, which were of great significance in guiding for the management of artificial forest ecosystem under the context of global change.

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EuRBG10 involved in indole alkaloids biosynthesis in Eucommia ulmoides induced by drought and salt stresses

Cited by 9 publications

References 33 publications

Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence

Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence

Exogenous Melatonin Strengthens Saline-alkali Stress Tolerance in M9-T337 Seedlings by Initiating a Variety of Physiological and Biochemical Pathways

Growth and non-structural carbohydrates response patterns of Eucommia ulmoides under salt and drought stress

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