Research Article Transfer of microsatellite markers from other Arecaceae species to <i>Syagrus</i> <i>romanzoffiana</i> (Arecaceae)

Abstract

The species Syagrus romanzoffiana, a native palm tree of South America, is widely distributed and well adapted to southern Brazil. It is an important economic, cultural, and ecological resource, being the preferred food of diverse animal species, involving complex ecological relationships. However, despite numerous molecular studies on native plants, specific molecular markers, such as expressed sequence tagsimple sequence repeats (EST-SSRs), are lacking, and there are few SSR markers for this species. Molecular data about individuals and their populations offers new perspectives for management, conservation, and economic exploitation. Here we tested 21 microsatellite loci known from three species of the family Arecaceae in three native populations of S. romanzoffiana in the state of Rio Grande do Sul, in southern Brazil. Among the 21 primer pairs tested, 12 produced an amplification product. Five loci were chosen and subjected to diversity tests. The populations presented between three and nine alleles per locus, and acceptable genetic diversity values; the expected heterozygosity ranged from 0.460 to 0.864, while the observed heterozygosity ranged from 0.100 to 1.000. We conclude that various microsatellite markers developed for other species of the family Arecaceae are suitable for molecular analysis of S. romanzoffiana populations.

Variation in palm tree plastidial simple sequence repeats, characterization, and potential use

“…The most abundant repetitive motifs found in the 52 species studied were AT and TTC/AAC/TAT for the di-and trimers, respectively. A total of 38,086 SSRs were found in the Arecaceae family in public domains (Palliyarakkal et al 2012), whereas 1,563 sequences were found in Syagrus romanzoffiana alone (Laindorf et al 2019). The most frequent motifs in these studies were di-and trimers.…”

“…In contrast, our study showed a higher number of SSRs comprising monomers. The discrepancy in the values found in different studies can be attributed to the type of data filtering used, as well as the increase in the amount of information available in databases, or even changes in the pattern of the strings (Laindorf et al 2019).…”

Transferibilidade De Marcadores Ssr, Diversidade E Estrutura Genética Em Syagrus Oleracea

“…Os amplicons para os marcadores transferidos apresentam tamanhos diferentes em comparação com os relatados na espécie original (LAINDORF et al, 2019), neste estudo os tamanhos dos fragmentos variaram de 50 a 350pb, diferindo dos tamanhos descritos na literatura. A diferença de tamanho entre os fragmentos amplificados permite detectar o polimorfismo, essa diferença se deve ao número de repetições dentro dos microssatélites (RALLO et al, 2000).…”

“…(NEIVA et al,2016). Outro estudo com a palmeira S. romanzoffiana em populações naturais coletadas no sul do Brasil a heterozigosidade observada também apresentou valores bem inferiores dos esperados (LAINDORF et al, 2019); já para palmeira S. coronata, estudo realizado com três populações no estado da Bahia encontrou média do número de alelos por locus no valor de 9,8 e heterozigosidade observada superior à esperada (SIMPLICIO et al, 2017), porém não foram descritas as condições de conservação das áreas em que foram coletadas as amostras.…”

Isolation of microsatellite markers for Copernicia prunifera (Miller) H. E. Moore (Arecaceae)

“…Also, they are easily obtained through PCR (polymerase chain reaction) assays, enabling rapid evaluation of many individuals for a large number of loci (Nazareno et al 2011). They can also be transferred between species of the same genus (Laindorf et al 2019), and once developed, can be shared between different laboratories.…”