Repurposing the Antibacterial Activity of Etoposide─A Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite

Ganesan, Vidhya; Gowri, M.; Devaraj, Muthu; Kandasamy, Sangeetha; Manogaran, Prasath; Kumar, G. Suresh; Govindan, R.; Kattimani, Vivekanand; Girija, E. K.

doi:10.1021/acsbiomaterials.1c01481

Cited by 7 publications

(5 citation statements)

References 66 publications

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“…Similarly, etoposide-A was repurposed for its anti-biofilm activity against S. aureus and has shown excellent activity in removing the biofilm which formed on hydroxyapatite. (34) Also, the drug penfluridol was repurposed for its antibacterial and antibiofilm activities against E. faecalis, (35) with promising results.…”

Section: Discussionmentioning

confidence: 99%

Repurposing of fluoxetine for antibacterial activities in catheter-associated urinary tract biofilm infections: an in vitro analysis

Poyil,

Shafqat,

Alfaki

2023

Univ Med

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BackgroundUrinary tract infections are often initiated by indwelling catheters and bring about serious consequences, especially when they are caused by multidrug-resistant bacterial pathogens. The biofilms of uropathogens such as Enterococcus faecalis and Escherichia coli pose serious challenges. Therefore the scientific world is trying to experiment with alternative drugs to replace conventional antibiotics as the latter are more prone to cause the development of antibacterial resistance. Here, we evaluate the repurposing of the antidepressant fluoxetine as an antibacterial agent against the mentioned pathogens. MethodsTo repurpose fluoxetine for its antibacterial activity against Enterococcus faecalis and Escherichia coli, the agar diffusion method was used. The minimal inhibitory concentration was found by the microdilution method. The drug was also analyzed as a coating on catheters to evaluate its efficiency against biofilm formation by pathogens. Results The drug fluoxetine showed potential antibacterial and anti-biofilm activities. Its minimum inhibitory concentration was found to be 18.75 µg/mL and 37.5 µg/mL against Enterococcus faecalis and Escherichia coli respectively. The antibiofilm activity on polystyrene surfaces was also remarkable as it reduced the formation of Enterococcus faecalis and Escherichia coli biofilms by 70% and 74%, after being treated with 1x MICs and 2x MICs respectively. Conclusions Fluoxetine - one of the drugs of choice in treating depression, when repurposed, has shown considerable antibacterial and antibiofilm effects against two of the major catheter-associated urinary tract infection-causing bacteria - viz. Enterococcus faecalis and Escherichia coli. Therefore, further studies are needed to understand its applicability as an antibacterial agent.

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Section: Discussionmentioning

confidence: 99%

Repurposing of fluoxetine for antibacterial activities in catheter-associated urinary tract biofilm infections: an in vitro analysis

Poyil,

Shafqat,

Alfaki

2023

Univ Med

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“…The antibacterial effect and controlled release of 3-methoxyazetidin-2-one were achieved by the colony forming unit (CFU) method. The number of colonies formed after an incubation of 24 h was counted by the hand counting method . All the bacterial experiments were performed in duplicate, and pure solvent served as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…The number of colonies formed after an incubation of 24 h was counted by the hand counting method. 46 All the bacterial experiments were performed in duplicate, and pure solvent served as a negative control.…”

Section: Effect Of Ph On the Size And Charge Behavior Of The Nanoform...mentioning

confidence: 99%

3-Methoxyazetidin-2-one Functionalized CuO–CB Microfibrils: A Drug Formulation with Controlled Release and Enhanced Synergistic Antibacterial Activities

Vijeata

Chaudhary

Bhalla

et al. 2023

ACS Appl. Bio Mater.

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trans-1-(4′-Methoxyphenyl)-3-methoxy-4-phenyl 3-methoxyazetidin-2-one (or 3-methoxyazetidin-2-one) is one of the important β-lactam derivatives with an ample range of bacterial activities yet few restrictions. To enhance the competency of the chosen 3-methoxyazetidin-2-one, microfibrils composed of copper oxide (CuO) and filter scraps of cigarette butts (CB) were chosen in the current work for developing a potential release formulation. The preparation of CuO-CB microfibrils required a simple reflux technique and a subsequent calcination treatment. The loading of 3-methoxyazetidin-2-one was processed via controlled magnetic stirring followed by centrifugation with microfibrils of CuO-CB. To confirm the loading efficiency, the 3methoxyazetidin-2-one@CuO-CB complex was analyzed by scanning electron microscopy, transmission electron microscopy, and infrared spectroscopy. Compared to the CuO nanoparticles, the release profile of CuO-CB microfibrils indicates only 32% of the drug release in the first 1 h at pH 7.4. As a model organism, E. coli has been utilized for in vitro drug release dynamic studies. Based on the observed drug release data, it was found that the prepared formulation evades premature drug release and triggers the on-demand release of drug inside bacterial cells. The controlled drug release by 3-methoxyazetidin-2-one@CuO-CB microfibrils over a period of 12 h further ascertained the excellent bactericide delivery mechanism to combat deadly bacterial resistance. Indeed, this study provides a strategy to combat antimicrobial resistance and eradicate bacterial disease via nanotherapeutics.

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“…Therefore, eggshell-derived HAP presented a great SSA and pore volume with good pore characteristics of the porous structure. It is an ideal candidate for various applications, such as adsorbents or solid substrates, to prepare heterogeneous catalysts [34,35]. The TEM technology was used to examine the surface crystal morphology of CuSO 4 @HAP, which further con rms the size of the nanoparticles and veri es its mesoporous structure.…”

Section: Characterization Of Cuso 4 @Hapmentioning

confidence: 99%

Fast catalytic degradation of organic dyes in aqueous media using copper sulfate deposited on biowaste-derived hydroxyapatite

Yarahmadi

Rezakazemi

Sheibani

et al. 2023

Preprint

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Conversion of biowastes into valuable products will assist sustainable development and waste management. Eggshell could be used as a bio-template for the synthesis of hydroxyapatite and widely applied in the field of catalytic processes. In this study, copper sulfate supported on hydroxyapatite (CuSO4@HAP) was rationally fabricated using biowaste eggshells (ES) by hydrothermal method and it was used for the fast catalytic degradation of organic pollutant dyes (OPDs) such as methylene blue (MB), 4-nitrophenol (4-NP) and 4-nitroaniline (4-NA) in the presence of sodium borohydride (NaBH4) in aqueous media. The physical and chemical properties of CuSO4@HAP were characterized via FTIR, XRD, BET, TEM, FE-SEM/EDS, and elemental mapping analyses. Results indicated that the synthesized catalyst was crystalline with great purity, homogeneous with fine particles, and mesoporous with a high specific surface area. Besides, the reaction progress was evaluated using a double-beam UV-vis spectrophotometer. The reaction conversion (%) under optimal conditions rapidly increased to an equilibrium state (99% for 4-NP, 96% for 4-NA, and 99.5% for MB) within a short time (t < 10 min). In addition, the catalytic rate constant of MB, 4-NP, and 4-NA in the presence of NaBH4 and CuSO4@HAP was calculated to be about 0.514, 0.492, and 0.317 min− 1, respectively. This study demonstrates the potential use of natural HAP-derived catalyst from biowaste eggshell with high efficiency and stability for degrading OPDs under mild reaction conditions. Additionally, CuSO4@HAP could be effectively separated and reused for four cycles with almost the same catalytic efficacy.

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Repurposing the Antibacterial Activity of Etoposide─A Chemotherapeutic Drug in Combination with Eggshell-Derived Hydroxyapatite

Cited by 7 publications

References 66 publications

Repurposing of fluoxetine for antibacterial activities in catheter-associated urinary tract biofilm infections: an in vitro analysis

Repurposing of fluoxetine for antibacterial activities in catheter-associated urinary tract biofilm infections: an in vitro analysis

3-Methoxyazetidin-2-one Functionalized CuO–CB Microfibrils: A Drug Formulation with Controlled Release and Enhanced Synergistic Antibacterial Activities

Fast catalytic degradation of organic dyes in aqueous media using copper sulfate deposited on biowaste-derived hydroxyapatite

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