Reproductive toxicity of Roundup WG® herbicide: impairments in ovarian follicles of model organism Danio rerio

Davico, Carla; Pereira, Alice Theresinha Cybis; Nezzi, Luciane; Jaramillo, Michael L.; Melo, Madson Silveira de; Müller, Yara Maria Rauh; Nazari, Evelise Maria

doi:10.1007/s11356-020-11527-z

Cited by 17 publications

(14 citation statements)

References 90 publications

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“…G and R at 10 µg/L had no impact on viability and proliferation of endometrial and myometrial luteal cells in cows at day 8 and 12 of the estrus cycle [37]. In Friesian ewes exposed from PND1 to PND14 to 2 mg/kg/bw/d of Roundup Full II ® , there was decreased endometrial cell proliferation (in the LE, the GE, the SS, and the myometrium) at PND45 accompanied by the inhibition of the production of an important protein for gland development: Foxa2 [34,40]. Moreover, no serum E2 variations were observed but Erα was downregulated in the LE, the GE, and the SS.…”

Section: In Utero and Perinatal Developmentmentioning

confidence: 98%

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

Serra

Estienne

Vasseur³

et al. 2021

Cells

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Glyphosate (G), also known as N-(phosphonomethyl)glycine is the declared active ingredient of glyphosate-based herbicides (GBHs) such as Roundup largely used in conventional agriculture. It is always used mixed with formulants. G acts in particular on the shikimate pathway, which exists in bacteria, for aromatic amino acids synthesis, but this pathway does not exist in vertebrates. In recent decades, researchers have shown by using various animal models that GBHs are endocrine disruptors that might alter reproductive functions. Our review describes the effects of exposure to G or GBHs on the hypothalamic–pituitary–gonadal (HPG) axis in males and females in terms of endocrine disruption, cell viability, and proliferation. Most of the main regulators of the reproductive axis (GPR54, GnRH, LH, FSH, estradiol, testosterone) are altered at all levels of the HPG axis (hypothalamus, pituitary, ovaries, testis, placenta, uterus) by exposure to GBHs which are considered more toxic than G alone due to the presence of formulants such as polyoxyethylene tallow amine (POEA).” In addition, we report intergenerational impacts of exposure to G or GBHs and, finally, we discuss different strategies to reduce the negative effects of GBHs on fertility.

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Section: In Utero and Perinatal Developmentmentioning

confidence: 98%

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

Serra

Estienne

Vasseur³

et al. 2021

Cells

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“…Pyriform cells are very rich in mitochondria owing to their nurse function [ 28 ] and, indeed, would represent an easy target for the herbicide. Mitochondrial effects have been already reported, in Danio [ 48 ] and mice [ 2 ], for example.…”

Section: Discussionmentioning

confidence: 99%

Glyphosate Interference in Follicular Organization in the Wall Lizard Podarcis siculus

Rosati

Chianese

Gregorio

et al. 2023

IJMS

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Glyphosate (Gly) is a broad-spectrum herbicide widely used thanks to its high efficiency and low toxicity. However, evidence exists of its toxic effects on non-target organisms. Among these, the animals inhabiting agricultural fields are particularly threatened. Recent studies demonstrated that exposure to Gly markedly affected the morphophysiology of the liver and testis of the Italian field lizard Podarcis siculus. The present study aimed to investigate the effects of the herbicide on the female reproductive system of this lizard in order to have a full picture of Gly-induced reproductive impairment. The animals were exposed to 0.05 and 0.5 μg/kg of pure Gly by gavage for 3 weeks. The results demonstrated that Gly, at both doses tested, profoundly interfered with ovarian function. It induced germ cells’ recruitment and altered follicular anatomy by anticipating apoptotic regression of the pyriform cells. It also induced thecal fibrosis and affected oocyte cytoplasm and zona pellucida organizations. At the functional levels, Gly stimulated the synthesis of estrogen receptors, suggesting a serious endocrine-disrupting effect. Overall, the follicular alterations, combined with those found at the level of the seminiferous tubules in males, suggest serious damage to the reproductive fitness of these non-target organisms, which over time could lead to a decline in survival.

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“…Moreover, the thickness of the follicular layer and the vitelline envelope were measured in the vitellogenic and previtellogenic oocyte II. Oocyte stage identification was based on the characteristics outlined in Lambert (1970), Selman et al (1993), and Davico et al (2021) and took into account the characteristics of the nucleus, cytoplasm, and yolk granules: Oogonium (OOG—the earliest germ cells, arranged in small clusters, each cell with a large nucleus and a small quantity of cytoplasm), previtellogenic oocyte I (PRE I—large nucleus with multiple nucleoli and a basophilic and homogeneous cytoplasm), previtellogenic oocyte II (PRE II—presence of yolk granules accumulated in the non‐homogeneous cytoplasm), vitellogenic oocyte (VIT—a large amount of yolk granules in the acidophilic cytoplasm, and a nondistinguishable nucleus), and atretic preovulatory oocyte (ATR—composed of degenerating oocyte presenting cell shrinkage and/or rupture of the yolk vesicles and other components of the germ cell cytoplasm).…”

Section: Methodsmentioning

confidence: 99%

In vivo exposure to pyriproxyfen causes ovarian oxidative stress and inhibits follicle maturation in zebrafish

Oliveira

Marins

Mendes

et al. 2023

J of Applied Toxicology

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We analyzed the effects of pyriproxyfen (PPF) on oxidative stress and ovarian morphology in zebrafish. PPF (10−9 M) exposure increased reactive oxygen species generation in ovaries, in association with a decrease in glutathione content. The activities of glutathione S‐transferase, superoxide dismutase, and catalase were increased, while γ‐glutamyltransferase activity was not altered by pesticide treatment. The histology of ovarian tissue showed an increase in the number of previtellogenic oocytes I, and a decrease in the rate of vitellogenic oocyte (VIT) count, suggesting inhibition of follicular maturation. An increase in the thickness of the vitelline envelope was observed in VIT, as was a tendency toward an increase in atresia in the ovary of the PPF‐treated group. These findings indicate that the deleterious effect of PPF on ovarian maturation is mediated by a redox imbalance and oxidative damage. So, PPF acts as an endocrine disruptor chemical and may compromise fish reproduction by reducing female fertility.

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Reproductive toxicity of Roundup WG® herbicide: impairments in ovarian follicles of model organism Danio rerio

Cited by 17 publications

References 90 publications

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

Glyphosate Interference in Follicular Organization in the Wall Lizard Podarcis siculus

In vivo exposure to pyriproxyfen causes ovarian oxidative stress and inhibits follicle maturation in zebrafish

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