Reproductive Outcomes After Vitrification of Human Blastocysts Coming From Three Different IVF Programs

Pérez-Albalá, Sonia; Santos, Ma José de los; Galán, Arancha; Zulategui, J.; Remohı́, J.; Escribá, Marı́a José

doi:10.1016/j.fertnstert.2005.07.429

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Electron Microscopy1

Vitrification and Warming Procedures1

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“…Thus, a total of 11 day-6 human blastocysts were included in the fine microscopic study. The group size difference was due to the expected loses as a result of vitrification, earlier estimated at 40% (24,25).…”

Section: Electron Microscopymentioning

confidence: 97%

“…Blastocyst vitrification was performed following our previously reported procedure (24,25), based on the method of Yokota and co-workers (26), with some minor modifications. In short, 16 day-5, abnormal blastocysts were placed for 2 minutes in 1 mL of Dulbecco phosphate-buffered saline solution supplemented with 20% (v/v) serum (hereafter s-PBS).…”

Section: Vitrification and Warming Proceduresmentioning

confidence: 99%

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