Repro‐protective role of royal jelly in phenylhydrazine‐induced hemolytic anemia in male mice: Histopathological, embryological, and biochemical evidence

Abstract

To estimate the repro‐protective effect of royal jelly (RJ) on phenylhydrazine (PHZ)‐induced anemia's detrimental effects, 24 mature mice were divided into control group (0.10 mL normal saline; intra‐peritoneally), RJ group (100 mg/kg/day; orally), experimental anemia (EA) group that received only PHZ (6 mg/100 g/48 h; intra‐peritoneally), and RJ + EA (according to the previous prescription) group. After 35 days, testicular histoarchitecture, RNA damage in germinal cells, sperm characteristics, testicular total anti‐oxidant capacity and malondialdehyde as well as serum testosterone levels, pre‐implantation embryo development and cyclin D1 and c‐myc mRNA levels at two‐cell, morula and blastocyst stages were analyzed. Spermatogenesis indices were ameliorated following RJ co‐administration. Moreover, RJ co‐treatment reduced germinal cells RNA damage, improved sperm characteristics, boosted pre‐implantation embryo development and restored androgenesis, and oxidant/anti‐oxidant status. Co‐administration of RJ also decreased mRNA levels of cyclin D1 and up‐regulated those of c‐myc in two‐cell embryos, morulas and blastocysts. The findings suggest that RJ can play a repro‐protective role in PHZ‐induced anemia in mice through anti‐oxidant defense system reinforcement and androgenesis restoration as well as cyclin D1 and c‐myc expressions regulation.

Antioxidant and Anti-Anemic Effects of Ethanol Leaf Extracts of Mucuna poggei and Telfairia occidentalis in Phenyl-Hydrazine-Induced Anemia in Wistar Albino Rats

“…MDA levels were reported to be higher during hepatotoxicity and hepatocarcinogenesis caused by PHZ. 28 In a different study, evaluation of MDA levels in serum revealed a significant rise in the PHZ group compared with the control group. Recent research has shown that one of the key mechanisms of PHZ in the development of hemolytic anemia is an increase in MDA concentrations.…”

Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

“…[3,34] The administration of Vit-C was capable of restoring the reduction in testis weight, probably by alleviating the testicular toxicity evoked by CTL. [34,39,40] Here, CTL administration resulted in a marked reduction in sperm count, viability, and motility and also contributed to a substantial increase in abnormal morphology. These morphological abnormalities were marked in the tail rather than the head, in accordance with previous research.…”

“…[18,44] These beneficial effects were primarily attributed to the antioxidant and testosterone booster properties of Vit-C that improve testicular performance. [10,17,40,44,45] Likewise, researchers reported a positive effect of Vit-C on sperm volume, motility, viability, morphology, and DNA integrity against arsenic-induced toxicity in animals and humans. [8,9,17,46] A recent cohort study also revealed positive relations between men's dietary intake of Vit-C and fertilization rate.…”

“…[ 3,34 ] The administration of Vit‐C was capable of restoring the reduction in testis weight, probably by alleviating the testicular toxicity evoked by CTL. [ 34,39,40 ]…”

Determination of the Effect of Oral Dosage of Labna Product Supplemented with Fe3O4 Conjugated with Chitosan Nanoparticles on Growth Parameters, Liver and Kidney Parameters in Anaemic Rats Induced by Phenylhydrazine

“…The animals were raised under the supervision of specialized veterinary staff, taking into account the aspect of hygiene. Experimental animals were distributed into six groups each one including five animals, that included: T1 healthy control group (without treatment, which was given water and food only for the duration of the experiment), T2 induced anaemia infection group (given 40 mg by subcutaneous intraperitoneal of phenyl hydrazine/kg of body weight each day for 48 hours) [11], T3 group animals given Labneh enrichment with 2.5 mg of Fe 3 O 4 -NPs ml/day, T4 group animals given Labneh enrichment with 2.5 mg of Ch-NPs ml/day, T5 group animals given Labneh enrichment with 2.5 mg of each Fe 3 O 4 -NPs conjugated with Ch-NPs ml/day, T6 group animals given Labneh enrichment with 2.5 mg of Hamvir ml/day. Diets and water were free of ad labtum given, and 0.1 mg of phenyl hydrazine was given for each kg of body weight animal by subcutaneous intraperitoneal throughout the experiment.…”