Determination of the Effect of Oral Dosage of Labna Product Supplemented with Fe₃O₄ Conjugated with Chitosan Nanoparticles on Growth Parameters, Liver and Kidney Parameters in Anaemic Rats Induced by Phenylhydrazine

Abstract

The research aims to investigate the effect of Fe3O4-NPs and its conjugated with chitosan nanoparticles on the rat’s body weights, liver enzymes, and the kidney function parameters in the serum of male albino rats induced with anaemia by phenyl-hydrazine. The rats were separated into six groups in five replicates, each of which included (T1) the control group, (T2) anaemia induced group, T3 group animals given Labneh enrichment with 2.5 mg of Fe3O4-NPs ml/day, T4 group animals given Labneh enrichment with 2.5 mg of Ch-NPs ml/day, T5 group animals given Labneh enrichment with 2.5 mg of each Fe3O4-NPs conjugated with Ch-NPs ml/day, T6 group animals given Labneh enrichment with 2.5 mg of Hamvir ml/day, and were fed for 30 days. The results showed a significant decrease (p≤0.05) in the Rat body weight gains of the anaemia-induced Rats group T2 (-1g) compared with the T1 group (22g). The liver enzyme concentration of AST, ALT and ALP was seen as a significant increase in the anaemia group T2 and became at 57.0, 62.6 and 82.0 compared to the T1 group at 38.3, 47.0 and 63.6 (IU/L) respectively. Also, the Creatinine and Urea of kidney function parameters were increased to 2.85 and 62.0 compared to the T1 group at 0.89 and 38.3 mg/dl respectively. The results of the oral dose of rats treatment groups T3, T4, and T5 and the treatment by drug Hamvir T6 were shown to significantly ameliorate the parameters of body weights, liver enzymes AST, ALT, ALP and kidney parameters of Creatinine and Urea parameters to its becomes at the values near of its in T1 value.