Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA

Wong, Wai Shing; Li, Geng Guang; Ning, Wen; Xu, Zeng-Fu; Hsiao, W.L. Wendy; Zhang, Lang Yin; Li, Ning

doi:10.1016/s0168-9452(01)00505-2

Cited by 47 publications

(24 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2B). Transformation positive percentage, the ratio of the number of Southern blotting positive shoots against number of total kanamycin resistant shoots, was 27.3 % in this study, which is lower than those reported for other citrus species, such as 90 % (Cervera et al 1998) and 88 % (Wong et al 2001) for citrange, or 87 % for P. trifoliata (Wong et al 2001). Low kanamycin concentration applied in this study might partially explain the high percentage of escape shoots.…”

contrasting

confidence: 78%

Establishment of an Agrobacterium-mediated transformation system for Fortunella crassifolia

Yang

Hu³

et al. 2007

Biol. plant.

View full text Add to dashboard Cite

Epicotyl segments of kumquat (Fortunella crassifolia Swingle cv. Jindan) were transformed with Agrobacterium tumefaciens GV3101 harboring neomycin phosphotransferase gene (npt II) containing plant expression vectors. Firstly, the explants were cultured in darkness at 25 °C on kanamycin free shoot regeneration medium (SRM) for 3 d, and then on SRM supplemented with 25 mg dm -3 kanamycin and 300 mg dm -3 cefotaxime for 20 d. Finally, they were subcultured to fresh SRM containing 50 mg dm -3 kanamycin monthly and grown under 16-h photoperiod. Sixty five kanamycin resistant shoots were regenerated from 500 epicotyl explants after four-month selection. Shoot tips of 20 strong shoots were grafted to 50-day-old kumquat seedlings and survival rate was 55 %. Among the 11 whole plants, 3 were transgenic as confirmed by Southern blotting. This is the first report on transgenic kumquat plants, and a transformation efficiency of 3.6 % was achieved.

show abstract

contrasting

confidence: 78%

Establishment of an Agrobacterium-mediated transformation system for Fortunella crassifolia

Yang

Hu³

et al. 2007

Biol. plant.

View full text Add to dashboard Cite

show abstract

“…There are a few reports about the introduction of genes of agronomic importance in Citrus, including the gene that encodes for the Citrus tristeza virus coat protein (Gutiérrez-E. et al, 1997;Domínguez et al, 2000;Yang et al, 2000), the HAL2 gene that confers tolerance to salinity (Cervera et al, 2000), LEAFY and APETALA1 genes that promote early flower initiation (Peña et al, 2001), and CS-ACS1 gene that controls the ethylene biosynthesis in Citrus (Wong et al, 2001). Reports on genetic transformation of the main cultivars used in the Brazilian citrus industry are very few and there are no reports of genetic transformation of the mostly used rootstock in Brazil, the Rangpur lime.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

Almeida

Filho

Mendes

et al. 2003

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck). Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod) were used as explants. These were cocultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with Agrobacterium suspension for 5, 10, 20 or 40 minutes; co-cultivation medium was supplemented with acetosyringone at 0, 100 or 200 µmol L -1; Explants ends had a longitudinal terminal incision (2-3 mm); co-cultivation temperatures of 19, 23 or 27°C were imposed. The experimental design was completely randomized in all experiments with five replications, each consisted of a Petri dish (100 x 15 mm) with 30 explants and resulted in a total of 150 explants per treatment. Longitudinal terminal incision in the explant ends did not improve shoot regeneration. However, transgenic plants of all three cultivars were confirmed from explants that had been subjected to inoculation time of 20 minutes, co-culture of three days at 23-27°C, in the absence of acetosyringone. Key words: genetic transformation, organogenesis, micrografting, improvement TRANSFORMAÇÃO GENÉTICA EM Citrus sinensis E Citrus limonia MEDIADA POR Agrobacterium tumefaciens A PARTIR DE SEGMENTOS DE EPICÓTILORESUMO: A transformação genética permite produzir cultivares com características específicas e pode, dessa forma ser associada a programas de melhoramento de citros. O objetivo deste trabalho foi estabelecer protocolos de transformação genética para as laranjas doce 'Valência' e 'Natal' (Citrus sinensis L. Osbeck), bem como para o limão 'Cravo'(Citrus limonia L. Osbeck). Segmentos de epicótilo de plântulas germinadas in vitro (três semanas no escuro e duas semanas sob fotoperíodo de 16h) foram utilizados como explantes. Estes foram co-cultivados com Agrobacterium tumefaciens (EHA-105), realizando-se vários experimentos para avaliar a eficiência do processo de transformação genética: explantes co-cultivados por um, três e cinco dias; tempo de inoculação com a bactéria de 5, 10, 20 e 40 minutos; co-cultivo em meio de cultura contendo 0, 100 e 200 µmol L -1 de acetoseringona; Incisão longitudinal (2-3 mm) nas extremidades do explante; temperatura de co-cultivo 19, 23 e 27°C. Todos os experimentos consistiram de cinco repetições por tratamento, sendo cada repetição representada por uma placa de Petri contendo 30 explantes, perfazendo um total de 150 explantes por tratamento. Plântulas transgênicas dos três cultivares foram obtidas utilizando-se tempo de inoculação de 20 minutos, co-cultivo com Agrob...

show abstract

“…For instance, a 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) gene promoted by ET was identified in melon (ACS1) (Gerchikov et al 2008) and citrus (CS-ACS1) (Wong et al 2011). In lettuce, Ls-ACS1 is induced by ET and auxin (Takahashi et al 2003).…”

Section: Research Articlementioning

confidence: 99%

Organic hydroponics induces systemic resistance against the air-borne pathogen,Botrytis cinerea(gray mould)

Chinta

Eguchi

Widiastuti

et al. 2015

Journal of Plant Interactions

View full text Add to dashboard Cite

Here, we propose that organic hydroponics trigger induced systemic resistance (ISR) in lettuce against air-borne Botrytis cinerea, which causes gray mold. We compared effects of organic and chemical hydroponics, assessed presence of ISR elicitors in the hydroponic nutrient solution, and investigated molecular mechanism of ISR. Organic hydroponics significantly reduced gray mold lesions in lettuce (cultivated hydroponically) and cucumber (cultivated in soil and foliar sprayed with nutrient solution). The 1-aminocyclopropane-1-carboxylic acid synthase gene in lettuce and lipoxygenase and ethylene receptor-related gene in cucumber showed heightened expression, suggesting that the jasmonic acid/ethylene (JA/ET)-signaling pathway was involved in ISR for both crops. Low salicylic acid β-glucoside levels confirmed role of the ISR signaling pathway. ISR in both lettuce and cucumbers indicated that elicitors in organic hydroponics were nonhost-specific and that the JA/ET pathway was activated without microbe-root interaction. Thus, organic hydroponics can be an effective method for both soil-borne and air-borne disease control.

show abstract

Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA

Cited by 47 publications

References 42 publications

Establishment of an Agrobacterium-mediated transformation system for Fortunella crassifolia

Establishment of an Agrobacterium-mediated transformation system for Fortunella crassifolia

Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

Organic hydroponics induces systemic resistance against the air-borne pathogen,Botrytis cinerea(gray mould)

Contact Info

Product

Resources

About