Repair of rabbit cartilage defect based on the fusion of rabbit bone marrow stromal cells and Nano-HA/PLLA composite material

Abstract

Objective To assess the effect of the fusion of rabbit bone marrow stromal cells (rBMSCs) and Nano-hydroxyapatite/poly (l-lactic acid) (Nano-HA/PLLA) in repairing the rabbit knee joint with full-thickness cartilage defect. Method The rBMSCs were isolated and cultured in vitro, and the third generation of rBMSCs was co-cultured with the Nano-HA/PLLA to construct the tissue-engineered cartilage (TEC). Eighteen New Zealand white rabbits were selected and randomly divided into three groups, namely, TEC group, Nano-HA/PLLA group, and control group. A cartilage defect model with the diameter of 4.5 mm and depth of 5 mm was constructed on the articular surface of medial malleolus of rabbit femur. General observation, histological observation, and Wakitani's histological scoring were conducted in the 12th and 24th week postoperatively. Results The results of TEC group indicated that new cartilage tissue was formed on the defect site and subchondral bone achieved physiological integration basically. Histological and immunohistochemical analyses indicated the generation of massive extracellular matrix. In contrast, limited regeneration and reconstruction of cartilage was achieved in the Nano-HA/PLLA group and control group, with a significant difference from the TEC group (p < 0.05). Moreover, the effect of cartilage repair was positively correlated with time. Conclusion The porous Nano-HA/PLLA combined with BMSCs promoted the repair of weight-bearing bone of adult rabbit's knee joint with cartilage defect.

Glycol chitosan/oxidized hyaluronic acid hydrogels functionalized with cartilage extracellular matrix particles and incorporating BMSCs for cartilage repair

“…Articular cartilage lacks an intrinsic capacity for self-regeneration because of avascular structure and no innervation tissue nature [1,2]. Currently, many treatment methods are conducted to address the problem of cartilage repairs, such as autografts and allografts.…”

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

“…Due to the high biocompatibility, strong toughness, and cell adhesive ability of nano-HAP, many kinds of literatures have reported that nano-HAP possessed effective repair capability for articular cartilage (Reddi et al, 2011;Zhou et al, 2015;Zhu et al, 2017). A magnetic nanocomposite hydrogel made of nano-HAP particles, Fe 2 O 3 nanoparticles, and PVA in a ratio of 1:0.5:10 was fabricated by using the ultrasonic dispersion approach and then lyophilized by a freeze-thawing crosslinking process, as shown in Figure 5A.…”

Microfluidic-based screening of resveratrol and drug-loading PLA/Gelatine nano-scaffold for the repair of cartilage defect

“…The density of resveratrol-loading PLA/Gelatine 3D nanofibre sponge was 92.68 mg/cm 3 , which was calculated by formula (1). The nanofibre sponges had good elasticity and mechanical strength and could play a certain role in supporting the repair of cartilage defect.…”

“…Articular cartilage defect is one of the most common diseases resulted from trauma, degeneration, inflammation, and neoplasm [1][2][3]. Due to the lack of blood supply and single cell component, it is difficult to cure after cartilage injury.…”