Repair of critical size defects in the rat cranium using ceramic‐reinforced PLA scaffolds obtained by supercritical gas foaming

Montjovent, Marc-Olivier; Mathieu, Laurence; Schmoekel, Hugo G.; Mark, Silke; Bourban, P.-E.; Zambelli, Pierre‐Yves; Laurent-Applegate, Lee Ann; Pioletti, Dominique P.

doi:10.1002/jbm.a.31208

Cited by 79 publications

(56 citation statements)

References 46 publications

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“…One advantage of combining ceramic and polymer is to control resorption rates [36]. In a pilot study in cranial defects in rats, the same PLA/ceramic scaffolds as used in this study did not show signs of alteration during the first 3 months after implantation (unpublished results), whereas fragmentation was observed after 18 weeks [6]. In the present work, and confirming these observations, changes in scaffold morphology were not noticed before 6 months post-surgery in the cranial model.…”

Section: Discussionmentioning

confidence: 63%

“…However, in the case of the PLA/TCP biocomposite, fetal bone cell development did not seem to be affected by differences in porosity and pore size in vitro [2]. In a preliminary in vivo study concerning PLA/TCP obtained by supercritical gas foaming, only one type of porous structure, porosity and pore size was tested and bone repair was assessed [6]. The aim of the present study was to evaluate the osteogenicity of fetal bone cells in vivo when associated to PLA/TCP scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, this technique allows producing biocompatible samples without the use of potentially toxic organic solvents [4,5]. These structures were recently tested in vivo for host tissue induced reactions as well as for their osteoconductive properties [6].…”

Section: Introductionmentioning

confidence: 99%

“…Modelling on parietal bone has been applied on rats from different strains and at various ages [10,[15][16][17][18]. Three months old Sprague-Dawley rats did not spontaneously fill 8 mm diameter lacunae 18 weeks post-surgery [6].…”

Section: Introductionmentioning

confidence: 99%

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Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

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Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promote bone repair in vivo when combined with porous scaffolds based on poly(L-lactic acid) (PLA) obtained by supercritical gas foaming and reinforced with 5 wt.% β-tricalcium phosphate (TCP).Bone regeneration was assessed by radiography and histology after implantation of PLA/TCP scaffolds alone, seeded with primary fetal bone cells, or coated with demineralized bone matrix. Craniotomy critical size defects and drill defects in the femoral condyle in rats were employed. In the cranial defects, polymer degradation and cortical bone regeneration were studied up to 12 months postoperatively. Complete bone ingrowth was observed after implantation of PLA/TCP constructs seeded with human fetal bone cells. Further tests were conducted in the trabecular neighborhood of femoral condyles, where scaffolds seeded with fetal bone cells also promoted bone repair.We present here a promising approach for bone tissue engineering using human primary fetal bone cells in combination with porous PLA/TCP structures. Fetal bone cells could be selected regarding osteogenic and immune-related properties, along with their rapid growth, ease of cell banking and associated safety.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

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“…246,247 Both PGA and PLA scaffolds has been investigated as a slowdelivery carrier for growth factors in several in vitro and in vivo studies, and demonstrated the ability to promote healing and osseointegration compared with control scaffolds. [248][249][250] However, due to the low modulus of PLA, it must be either copolymerized with a higher modulus polymer, or made into a composite with a different material. PGA, the simplest linear aliphatic polyester is highly crystalline (45-55%), has a high melting point (220 C), and a glass transition temperature of $35 C. PGA alone has a high modulus (7 GPa), and completely degrades in vivo within 4-6 months.…”

Section: Mechanisms Of Polymer Biodegradationmentioning

confidence: 99%

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Porter

Ruckh

Popat

2009

Biotechnology Progress

672

499

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Critical‐sized defects in bone, whether induced by primary tumor resection, trauma, or selective surgery have in many cases presented insurmountable challenges to the current gold standard treatment for bone repair. The primary purpose of a tissue‐engineered scaffold is to use engineering principles to incite and promote the natural healing process of bone which does not occur in critical‐sized defects. A synthetic bone scaffold must be biocompatible, biodegradable to allow native tissue integration, and mimic the multidimensional hierarchical structure of native bone. In addition to being physically and chemically biomimetic, an ideal scaffold is capable of eluting bioactive molecules (e.g., BMPs, TGF‐βs, etc., to accelerate extracellular matrix production and tissue integration) or drugs (e.g., antibiotics, cisplatin, etc., to prevent undesired biological response such as sepsis or cancer recurrence) in a temporally and spatially controlled manner. Various biomaterials including ceramics, metals, polymers, and composites have been investigated for their potential as bone scaffold materials. However, due to their tunable physiochemical properties, biocompatibility, and controllable biodegradability, polymers have emerged as the principal material in bone tissue engineering. This article briefly reviews the physiological and anatomical characteristics of native bone, describes key technologies in mimicking the physical and chemical environment of bone using synthetic materials, and provides an overview of local drug delivery as it pertains to bone tissue engineering is included. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009

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Testing Natural Biomaterials in Animal Models

Costa-Pinto¹,

Santos²,

Neves³

et al. 2016

Biomaterials From Nature for Advanced Devices and Therapies

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Animal models have been extensively developed in the last decades in biomedical field. Their use has shown particular relevance in fields such as cell biology, genetics, anatomy and development, biochemistry, infection and immunity, cancer research, drugs and vaccine development, tissue engineering and regenerative medicine.Despite major advances regarding in vitro models aiming to mimic the complexity and cellular interaction existing within tissues [1-3], in vivo testing is essential to safely investigate the biological performance of newly developed devices when implanted in a living system. A better characterization of such response at cellular and molecular level is required, and has been extensively investigated in the last decades [4][5][6][7]. However, the complexity of in vivo responses to implanted biomaterials renders this assessment a challenging issue to address. * Both authors equally contributed for the writing of this book chapter.Biomaterials from Nature for Advanced Devices and Therapies, First Edition. Edited by Nuno M. Neves and Rui L. Reis.

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Repair of critical size defects in the rat cranium using ceramic‐reinforced PLA scaffolds obtained by supercritical gas foaming

Cited by 79 publications

References 46 publications

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Testing Natural Biomaterials in Animal Models

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