Testing Natural Biomaterials in Animal Models

Costa-Pinto, Ana Rita; Santos, T. C.; Neves, Nuno M.; Reis, Rui L.

doi:10.1002/9781119126218.ch30

Cited by 10 publications

(5 citation statements)

References 127 publications

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“…ECM scaffolds, as dHCM, are envisioned to help and accelerate the remodeling of injured tissues in vivo [ 47 ]. By its subcutaneous implantation in animal models, it is possible to obtain information about the elicited inflammatory response towards the biomaterial implanted and about its integration with the host tissue [ 48 , 49 ]. In summary, a healthy host response after biomaterial implantation is characterized by an acute proinflammatory phase mainly orchestrated by neutrophils, monocytes and macrophages, followed by an anti-inflammatory phase mediated by the transition from M1 to M2 macrophages.…”

Section: Discussionmentioning

confidence: 99%

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration

et al. 2020

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Although some placenta-derived products are already used for tissue regeneration, the human chorion membrane (HCM) alone has been poorly explored. In fact, just one study uses decellularized HCM (dHCM) with native tissue architecture (i.e., without extracellular matrix (ECM) suspension creation) as a substrate for cell differentiation. The aim of this work is to fully characterize the dHCM for the presence and distribution of cell nuclei, DNA and ECM components. Moreover, mechanical properties, in vitro biological performance and in vivo biocompatibility were also studied. Our results demonstrated that the HCM was successfully decellularized and the main ECM proteins were preserved. The dHCM has two different surfaces, the reticular layer side and the trophoblast side; and is biocompatible both in vitro and in vivo. Importantly, the in vivo experiments demonstrated that on day 28 the dHCM starts to be integrated by the host tissue. Altogether, these results support the hypothesis that dHCM may be used as a biomaterial for different tissue regeneration strategies, particularly when a membrane is needed to separate tissues, organs or other biologic compartments.

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Section: Discussionmentioning

confidence: 99%

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration

et al. 2020

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“…In this research, the results revealed through these validated procedures that this unique MA-Kefiran hydrogel should be evaluated, in the future, in animal models, a step midway between in vitro studies and human clinical trials [57]. The in vivo assessment of the developed hydrogel on OA animal models is currently being performed.…”

Section: Biocompatibility Of Ma-kefiran Extractsmentioning

confidence: 98%

Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications

et al. 2021

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Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications.

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“…The foreign body response to biomaterials usually occurs at the implant site with prolonged pro-inflammatory activity. Proinflammatory reactions, M1 cell activation, formation of multinucleated foreign body giant cells and granulomatous inflammation eventually interfere with the mechanical properties of the material and lead to material destruction 31,32 characterized adverse chronic reaction. As seen in Fig.…”

Section: Discussionmentioning

confidence: 99%