Repair of critical‐size bone defects using bone marrow stromal cells: a histomorphometric study in rabbit calvaria. Part I: Use of fresh bone marrow or bone marrow mononuclear fraction

Abstract

Both methods using bone marrow stromal cells contributed to enhancing bone healing, especially that using the bone marrow mononuclear fraction. The use of a barrier membrane seemed to have a synergistic effect.

Critical size defects for bone regeneration experiments in rabbit calvariae: systematic review and quality evaluation using ARRIVE guidelines

“…In ARRIVE grading, the maximum possible score is 36 points, but the highest score in the present review only reached 29 points for quality (Pelegrine et al. ). Furthermore, the differences between the maximum possible score (reporting quality) vs. the value achieved showed that none of the categories met the necessary quality needed to compare or reproduce future work.…”

“…; Pelegrine et al. ) also showed incomplete healing at all times. These findings are due to the selection of defect size, which, according with the results of the present review, is critical for each specific experimental procedure as follows:…”

“…Critical size defects of 15 mm diameter and supra-critical defects (18, 20 and 17 9 17 mm) showed incomplete healing in all studies (Moghadam et al 2004;Jan et al 2006;Tuusa et al 2008;Nagata et al 2009;Peters & Courtemanche 2009;Ma et al 2010;Sohn et al 2010;Azevedo-Neto et al 2012;Dumas et al 2012;Faratzis et al 2012;Gomes et al 2012;Oliveira et al 2012;Hassanein et al 2013), at different experimental times, while subcritical defects (6, 8, 10, 11, 10 9 10, 15 9 10, 15 9 13 and 20 9 10 mm; Clokie et al 2002;Haddad et al 2006;Pripatnanont et al 2007Pripatnanont et al , 2009Tamimi et al 2008;Ereno et al 2010;Humber et al 2010;Sohn et al 2010;Borie et al 2011;Behfarnia et al 2012;Pelegrine et al 2013) also showed incomplete healing at all times. These findings are due to the selection of defect size, which, according with the results of the present review, is critical for each specific experimental procedure as follows:…”

“…; Pelegrine et al. ). Defects of 10 mm could be critical beyond 8 weeks (Tamimi et al. ). Defects of 11 mm are critical up to 12 weeks (Sohn et al.…”

Bone healing after biodegradable mini-plate fixation

“…It is known that the rabbit metabolism is three times faster than that of humans 18 . Thus, the healing periods of four and 12 weeks were chosen for the comparisons because they were long enough to see the effects of the resorbable system on bone formation, such as bone incorporation, resorption of materials, bone remodeling, and amount of bone regeneration.…”