Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft

Sadeghi, Rokhsareh; Najafi, Mohammad Taghi Mashkouri; Semyari, Hassan; Mashhadiabbas, Fatemeh

doi:10.1007/s00056-016-0067-8

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…Autogenous grafts integrate faster, and the volume of bone seen for SS and MS groups at 2 months was seen within one month for particulate autogenous graft in one study. 28 However the values obtained in this study were slightly lower and higher to those observed by Takauti 29 (78%) and Sadeghi 30 (23%) in rabbits at 2 and 3 months for ceramics and nano-hydroxyapatite respectively. The possibility of thermal damage to the material appears remote in this study.…”

Section: Discussioncontrasting

confidence: 52%

Solving the Variable-Particle Size Quandary of Bone Mills. Development of an Automated Milling System to Generate Graft Particles of Definite Sizes

Chandra¹

2023

Preprint

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There are situations where block grafts have to be milled to convert them into particulate grafts of definite sizes. The objectives of this study were to evaluate the quality of graft particles generated in two sizes from a custom-built automated milling system (AMS) and their biocompatibility in an animal model. A Monetite block was milled in an AMS to generate small (SS group; 0.5-0.8 mm) and medium size (MS group; 1.0-1.2 mm) particles. Measures of particle count, Ferets diameter (dF), particle distribution and size were recorded. Biocompatibility of particles was tested in a rabbit tibial defect model. The average particle size was significantly smaller in the SS group than the MS group. There were significant to highly significant differences between SS and MS groups in measures of particle count. SS and MS groups had maximum percentage of particles in the 0.6-1mm (71%) and >1mm (70%) ranges respectively. The device was able to generate small and medium-size graft particles which were distinct from each other.

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Section: Discussioncontrasting

confidence: 52%

Solving the Variable-Particle Size Quandary of Bone Mills. Development of an Automated Milling System to Generate Graft Particles of Definite Sizes

Chandra¹

2023

Preprint

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“…Compared with the traditional HA, nanohydroxyapatite (nHA) has a larger surface area and better bioactivity and loading capacity. There were sufficient preclinical and clinical findings to support the osteogenic effect of nHA in tissue engineering (Shirmohammadi et al, 2014;Kamboj et al, 2016;Dayashankar et al, 2017;Sadeghi et al, 2017). Chitosan has been used for periodontal treatments in many studies.…”

Section: Discussionmentioning

confidence: 99%

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Liao

Shu

et al. 2020

Front. Cell. Infect. Microbiol.

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The recovery of impaired periodontium is still a challenge to the treatment of periodontitis. This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration. The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration. The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis. Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo. The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 µg/mL rhAm. Tryptic Soy Broth culture medium was used as a baseline control. Osteogenic effect of rhAm (20 µg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 µg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media. The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control. In the animal model, 4week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo. The root slices were treated with rhAm (20 µg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 µg/mL rhAm). The root slices treated with osteogenic medium alone were used as the baseline control. The analyses showed that the mHA/CS particles were 2 µm in diameter and had a uniform pore size. Liao et al. A Research on Periodontal Regeneration The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m 2 /g. The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis. The mHA/CS scaffold sustainably released rhAm. The mHA/CS loaded with 20 µg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro. Additionally, it promoted the formation of cementum-like tissue in vivo. Our findings suggest that mHA/CS loaded with 20 µg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.

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“…The field of nanotechnology can be applied to scaffolds such as HA to create nHA, which was shown to enhance bone regeneration and to accelerate healing after sinus augmentation [60]. In fact, nHA displayed similar effects in bone regeneration compared to freeze dried bone allografts in a rabbit model [63]. In addition, nHA used in the augmentation of extraction sockets was shown to reduce the degree of gingival invaginations [64].…”

Section: Bone Regeneration For Dental Implants and Mandibular Defectsmentioning

confidence: 99%

Nanomaterials in Craniofacial Tissue Regeneration: A Review

Tao

Pham

et al. 2019

Applied Sciences

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Nanotechnology is an exciting and innovative field when combined with tissue engineering, as it offers greater versatility in scaffold design for promoting cell adhesion, proliferation, and differentiation. The use of nanomaterials in craniofacial tissue regeneration is a newly developing field that holds great potential for treating craniofacial defects. This review presents an overview of the nanomaterials used for craniofacial tissue regeneration as well as their clinical applications for periodontal, vascular (endodontics), cartilage (temporomandibular joint), and bone tissue regeneration (dental implants and mandibular defects). To enhance periodontal tissue regeneration, nanohydroxyapatite was used in conjunction with other scaffold materials, such as polylactic acid, poly (lactic-co-glycolic acid), polyamide, chitosan, and polycaprolactone. To facilitate pulp regeneration along with the revascularization of the periapical tissue, polymeric nanofibers were used to simulate extracellular matrix formation. For temporomandibular joint (cartilage) engineering, nanofibrous-type and nanocomposite-based scaffolds improved tissue growth, cell differentiation, adhesion, and synthesis of cartilaginous extracellular matrix. To enhance bone regeneration for dental implants and mandibular bone defects, nanomaterials such as nanohydroxyapatite composite scaffolds, nanomodified mineral trioxide aggregate, and graphene were tested. Although the scientific knowledge in nanomaterials is rapidly advancing, there remain many unexplored data regarding their standardization, safety, and interactions with the nanoenvironment.

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Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft

Abstract: Based on the results of this experimental study, both NanoBone and FDBG exhibited a similar effect on bone formation.

Cited by 14 publications

References 39 publications

Solving the Variable-Particle Size Quandary of Bone Mills. Development of an Automated Milling System to Generate Graft Particles of Definite Sizes

Solving the Variable-Particle Size Quandary of Bone Mills. Development of an Automated Milling System to Generate Graft Particles of Definite Sizes

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Nanomaterials in Craniofacial Tissue Regeneration: A Review

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