Repair of alveolar cleft defect with mesenchymal stem cells and platelet derived growth factors: A preliminary report

Behnia, Hossein; Khojasteh, Arash; Soleimani, Masoud; Tehranchi, Azita; Atashi, Amir

doi:10.1016/j.jcms.2011.02.003

Cited by 151 publications

(115 citation statements)

References 28 publications

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“…Our results matched with Behania et al [18] study although short time evaluation of this study. In the opposite side, Khojasteh et al [17] concluded that mesenchymal stem cells enhance bone regeneration in critical size defects more than platelet derived growth factors.…”

Section: Discussionsupporting

confidence: 93%

“…The palatal mucoperiosteal flaps were reflected and sutured to each other to repair the palatal mucosa of palatal side of the cleft if needed. During preparation of the recipient site, in group I the required amount of blood was aspirated and centrifuged for PRF preparation according to Choukroun's method [17][18][19] and beside that the required amount of bone marrow was aspirated from the ilium. PRF gel (4-6 ml) and bone marrow aspirate (1-2 ml) were mixed with a mount of β-Tricalcium phosphate granules, according to size of defect and placed in recipient bed.…”

Section: Operative Proceduresmentioning

confidence: 99%

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Effect of Platelet-Rich Fibrin on The Regeneration Capacity of Bone Marrow Aspirate In Alveolar Cleft Grafting (Clinical and Radiographic Study)

Al-Ashmawy¹,

Ali²,

Baiomy³

2017

Dentistry

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The present study aimed to evaluate the enhancing effect of platelet-rich fibrin (PRF) on autogenous bone marrow aspirate (BMA) using in a resorbable matrix for repair of alveolar cleft defects. It was conducted on 12 patients (7 males and 5 females) with unilateral alveolar cleft and oronasal leakage. They divided into two random equal groups (group I and II). In group I, alveolar cleft defect packed with a mixture of PRF, β-Tricalcium phosphate, and BMA, and the same mixture without PRF for group II. Our clinical results revealed that, all patients had uneventful wound healing except some cases in group II. While, densitometric analysis showed a high statistical significant difference between two groups (p=0.011737) at 6 months interval but, at 12 months interval difference was not statistically significant (p=0.142480). Also, volumetric measurements of new bone showed that, there was highly statistical significant difference (p=0.037997) between group I and II at 12 month interval. The present study concluded that PRF may enhance the regeneration capacity of the stem cells when it added to BMA. Thereby, they may accelerate bone regeneration in alveolar cleft defects. Moreover, it may be used as membrane to cover grafted alveolar defect.

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Section: Discussionsupporting

confidence: 93%

Section: Operative Proceduresmentioning

confidence: 99%

Effect of Platelet-Rich Fibrin on The Regeneration Capacity of Bone Marrow Aspirate In Alveolar Cleft Grafting (Clinical and Radiographic Study)

Al-Ashmawy¹,

Ali²,

Baiomy³

2017

Dentistry

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“…Moy, Lundgren, and Holmes (1993) found that bone graft substitute materials implanted alone instead of autologous bone graft resulted in a lower success rate compared with the combination of cells and bone graft substitute. This suggests that tissue engineering strategies using cells combined with a supportive biomaterial scaffold may be a more effective treatment for cleft palate repair than biomaterials alone (Behnia, Khojasteh, Soleimani, Tehranchi, & Atashi, 2012; Khojasteh, Eslaminejad, & Nazarian, 2008). …”

Section: Introductionmentioning

confidence: 99%

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Puwanun

Delaine-Smith

Colley

et al. 2017

J Tissue Eng Regen Med

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Biodegradable electrospun polycaprolactone scaffolds can be used to support bone‐forming cells and could fill a thin bony defect, such as in cleft palate. Oscillatory fluid flow has been shown to stimulate bone production in human progenitor cells in monolayer culture. The aim of this study was to examine whether bone matrix production by primary human mesenchymal stem cells from bone marrow or jaw periosteal tissue could be stimulated using oscillatory fluid flow supplied by a standard see‐saw rocker. This was investigated for cells in two‐dimensional culture and within electrospun polycaprolactone scaffolds. From day 4 of culture onwards, samples were rocked at 45 cycles/min for 1 h/day, 5 days/week (rocking group). Cell viability, calcium deposition, collagen production, alkaline phosphatase activity and vascular endothelial growth factor secretion were evaluated to assess the ability of the cells to undergo bone differentiation and induce vascularisation. Both cell types produced more mineralized tissue when subjected to rocking and supplemented with dexamethasone. Mesenchymal progenitors and primary human mesenchymal stem cells from bone marrow in three‐dimensional scaffolds upregulated mineral deposition after rocking culture as assessed by micro‐computed tomography and alizarin red staining. Interestingly, vascular endothelial growth factor secretion, which has previously been shown to be mechanically sensitive, was not altered by rocking in this system and was inhibited by dexamethasone. Rocker culture may be a cost effective, simple pretreatment for bone tissue engineering for small defects such as cleft palate.

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“…The potential of these cells has evoked great enthusiasm and high expectations in both scientific and commercial circles. However, in general clinical applications of stem cells in reconstructive surgery have been limited to small case series with varying results and/or short follow‐up periods 14, 31, 32, 33. Among the published studies, there is a great variability in, for example, stem cell sources, defect sites and sizes, indications, cell carriers, and use of growth factors that makes it difficult to draw a solid conclusion on stem cell effectiveness in bone tissue engineering 26.…”

Section: Discussionmentioning

confidence: 99%

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

Thesleff

Lehtimäki

Niskakangas

et al. 2017

Stem Cells Translational Medicine

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Several alternative techniques exist to reconstruct skull defects. The complication rate of the cranioplasty procedure is high and the search for optimal materials and techniques continues. To report long‐term results of patients who have received a cranioplasty using autologous adipose‐derived stem cells (ASCs) seeded on beta‐tricalcium phosphate (betaTCP) granules. Between 10/2008 and 3/2010, five cranioplasties were performed (four females, one male; average age 62.0 years) using ASCs, betaTCP granules and titanium or resorbable meshes. The average defect size was 8.1 × 6.7 cm2. Patients were followed both clinically and radiologically. The initial results were promising, with no serious complications. Nevertheless, in the long‐term follow‐up, three of the five patients were re‐operated due to graft related problems. Two patients showed marked resorption of the graft, which led to revision surgery. One patient developed a late infection (7.3 years post‐operative) that required revision surgery and removal of the graft. One patient had a successfully ossified graft, but was re‐operated due to recurrence of the meningioma 2.2 years post‐operatively. One patient had an uneventful clinical follow‐up, and the cosmetic result is satisfactory, even though skull x‐rays show hypodensity in the borders of the graft. Albeit no serious adverse events occurred, the 6‐year follow‐up results of the five cases are unsatisfactory. The clinical results are not superior to results achieved by conventional cranial repair methods. The use of stem cells in combination with betaTCP granules and supporting meshes in cranial defect reconstruction need to be studied further before continuing with clinical trials. Stem Cells Translational Medicine 2017;6:1576–1582

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Repair of alveolar cleft defect with mesenchymal stem cells and platelet derived growth factors: A preliminary report

Cited by 151 publications

References 28 publications

Effect of Platelet-Rich Fibrin on The Regeneration Capacity of Bone Marrow Aspirate In Alveolar Cleft Grafting (Clinical and Radiographic Study)

Effect of Platelet-Rich Fibrin on The Regeneration Capacity of Bone Marrow Aspirate In Alveolar Cleft Grafting (Clinical and Radiographic Study)

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

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