Repair Kinetics of the Stratum corneum under Repeated Insults

Uhoda, Emmanuelle; Franchimont, Claudine; Debatisse, B.; Wang, X.; Pierard, Gérald

doi:10.1159/000083463

Cited by 21 publications

(18 citation statements)

References 71 publications

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“…have been employed in formulations as a means to alter the barrier properties of the stratum corneum layer of skin and aid in better permeation of the active agent. These permeation enhancers tend to disrupt the lipid structure of the SC layer, thereby causing damage which takes a long recovery time (22). Physical enhancement techniques like iontophoresis, sonophoresis, and microdermabrasion overcome the stratum corneum barrier effectively.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Microchannels Created by Metal Microneedles: Formation and Closure

Kalluri

Kolli²,

Banga

2011

AAPS J

120

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Abstract. Transdermal delivery of therapeutic agents for cosmetic therapy is limited to small and lipophilic molecules by the stratum corneum barrier. Microneedle technology overcomes this barrier and offers a minimally invasive and painless route of administration. DermaRoller ® , a commercially available handheld device, has metal microneedles embedded on its surface which offers a means of microporation. We have characterized the microneedles and the microchannels created by these microneedles in a hairless rat model, using models with 370 and 770 μm long microneedles. Scanning electron microscopy was employed to study the geometry and dimensions of the metal microneedles. Dye binding studies, histological sectioning, and confocal microscopy were performed to characterize the created microchannels. Recovery of skin barrier function after poration was studied via transepidermal water loss (TEWL) measurements, and direct observation of the pore closure process was investigated via calcein imaging. Characterization studies indicate that 770 μm long metal microneedles with an average base width of 140 μm and a sharp tip with a radius of 4 μm effectively created microchannels in the skin with an average depth of 152.5±9.6 μm and a surface diameter of 70.7±9.9 μm. TEWL measurements indicated that skin regains it barrier function around 4 to 5 h after poration, for both 370 and 770 μm microneedles. However, direct observation of pore closure, by calcein imaging, indicated that pores closed by 12 h for 370 μm microneedles and by 18 h for 770 μm microneedles. Pore closure can be further delayed significantly under occluded conditions.

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Section: Resultsmentioning

confidence: 99%

Characterization of Microchannels Created by Metal Microneedles: Formation and Closure

Kalluri

Kolli²,

Banga

2011

AAPS J

120

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“…Experimentally, tape stripping compromises the barrier function [10][11][12] , and the recovery rate of TEWL refl ects its repair kinetics [13][14][15] . Surfactants also alter barrier function [15,16] .…”

mentioning

confidence: 99%

“…The aim of the present study was to assess the effects of paste formulations on skin barrier repair after controlled tape strippings and sustained surfactant challenge following a previously described design [15] . Attention was paid to the amount of the products applied to the compromised skin, and to the infl uence, if any, of miconazole nitrate incorporated in the formulation.…”

mentioning

confidence: 99%

Dynamics of Skin Barrier Repair following Topical Applications of Miconazole Nitrate

Xhauflaire-Uhoda

Vroome²,

Cauwenbergh³

et al. 2006

Skin Pharmacol Physiol

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The skin barrier function (SBF) is an important aspect of skin biology, particularly in the elicitation of inflammation. The SBF recovery rate after tape stripping and surfactant challenge can be assessed by measuring the transepidermal water loss (TEWL). Previous clinical studies have shown some inflammatory effect after topical applications of miconazole. The aim of this study was to compare the effect of pastes (petrolatum and 15% zinc oxide) containing or not miconazole nitrate on controlled impaired SBF. Fifteen volunteers were enrolled. In each subject, successive cyanoacrylate skin surface strippings were harvested from 5 sites of the volar forearm until TEWL raised above 15 g/cm²/h on all test sites. In addition, one daily soak session with a 0.2% dishwashing liquid further damaged the SBF. Each of the test formulations was applied twice daily for 5 days at two dosages, namely 1 and 2 mg/cm², on randomized test sites. Another site remained untreated. TEWL was measured daily for 5 days. A fastened SBF repair was observed on all treated sites, particularly where the largest amount of the products had been applied. A faster SBF recovery rate was obtained at the site receiving the miconazole nitrate paste. We conclude that the occlusive effect of a paste helped mitigate SBF defect. The adjunction of miconazole nitrate improved the efficacy.

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“…In addition, TEWL was measured using a Tewameter® (C+K electronic) [7, 16, 17]. The Tewameter® recorded values after 30 s contact of the probe with the skin [17].…”

Section: Methodsmentioning

confidence: 99%

“…The Tewameter® recorded values after 30 s contact of the probe with the skin [17]. Values were expressed as g/m 2 /h.…”

Section: Methodsmentioning

confidence: 99%

Dew Point Effect of Cooled Hydrogel Pads on Human Stratum Corneum Biosurface

2007

Self Cite

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Background: Cooled hydrogel pads are used to prevent overheating effects of laser therapy. They do not induce cold injuries to the skin, but their more subtle physiological effects have not been thoroughly studied. Purpose: To describe the changes in transepidermal water loss and electrometric properties of the skin surface following application of cooled hydrogel pads. Measurements were performed on normal forearm skin of 27 healthy volunteers and on freshly excised skin from abdominoplasty. Methods: LaserAid® hydrogel pads cooled to 4°C were placed for 15 min on the forearm skin. Measurements of transepidermal water loss (TEWL) and electrometric properties (Corneometer®, Nova DPM 900®) were performed before application and after removal of the cooled pads. Results: A consistent increase in corneometer units, dermal phase meter (DPM) values and TEWL were recorded at removal of the cooled hydrogel pads. Both the in vivo and in vitro assessments brought similar information. Discussion: The similar changes disclosed in vitro and in vivo suggest that a common physical process is operating in these conditions. The observed phenomenon is opposite to the predicted events given by the Arrhenius law probably because of the combination of cooling and occlusion by the pads. A dew point effect (air temperature at which relative humidity is maximal) is likely involved in the moisture content of the stratum corneum. Thus, the biological impact of using cooling hydrogel pads during laser therapy is different from the effect of a cryogenic spray cooling procedure. The better preservation of the water balance in the stratum corneum by the cooled hydrogel pads could have a beneficial esthetic effect on laser treated areas.

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Repair Kinetics of the Stratum corneum under Repeated Insults

Cited by 21 publications

References 71 publications

Characterization of Microchannels Created by Metal Microneedles: Formation and Closure

Characterization of Microchannels Created by Metal Microneedles: Formation and Closure

Dynamics of Skin Barrier Repair following Topical Applications of Miconazole Nitrate

Dew Point Effect of Cooled Hydrogel Pads on Human Stratum Corneum Biosurface

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