Reovirus‐induced tenosynovitis in chickens the influence of age at infection

Jones, Richard C.; Georgiou, K.

doi:10.1080/03079458408418546

Cited by 57 publications

(24 citation statements)

References 20 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…age-related resistance to infection 23,24 . Young animals are more susceptible to ARV infections probably because their immune system is not yet fully developed 25 .…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Avian Reovirus Infections in Chickens in Western Provinces of Turkey

Erol¹,

Şengül²

2012

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

SummaryIn this study, seroprevalence of avian reovirus (ARV) infections in broiler and broiler breeder chicken farms in Aydin, İzmir and Manisa provinces were investigated. Serum samples (n = 631) collected from 8 broiler breeder and 18 broiler flocks were tested for ARV antibodies using a commercially available ARV Enzyme Linked Immunosorbent Assay (ELISA) kit. The sampled flocks were not vaccinated against ARV. The blood samples were taken at the time of slaughtering. Antibodies were found in all flocks tested; 224 of the 295 samples (75.93%) collected from broiler and 322 of the 336 samples (95.83%) collected from the broiler breeder chickens were seropositive. In total, 546 of the 631 serum samples (86.53%) were seropositive. Lameness and respiratory system disorders were observed in all of the broiler flocks but none of the broiler breeder flocks. Seropositivity in Izmir province (91.48%) was higher than seropositivity in Manisa and Aydin provinces (74.59 and 70.58%, respectively). These results suggest that ARV infections which may cause lameness, serious respiratory system disorders, and decreased productivity in broilers are very common in chickens in the Aegian region of Turkey. The infection is often subclinical in broiler breeders. These results highlight the need for regular implementation of programs to combat the ARV infection in western Turkey. Seropozitiflik oranlarının İzmir ilinde (%91.48), Manisa ve Aydın illerine nazaran daha yüksek olduğu tespit edildi (sırasıyla %74.59 ve %70.58). Sonuç olarak, broiler yetiştiriciliğinde önemli solunum sistemi semptomlarına, topallığa ve verim düşüklüklerine yol açan reovirusların Ege Bölgesi'nde çok yaygın olduğu görüldü. Özellikle damızlık sürülerde enfeksiyonun yüksek prevalansa sahip olduğu ve çoğu zaman semptom göstermeksizin, gizli bir şekilde devam ettiği belirlendi. Bu araştırmanın sonuçlarına dayanarak, söz konusu virus enfeksiyonuna karşı düzenli mücadele programlarının hazırlanması ve uygulanması gerektiği sonucuna varıldı.

show abstract

“…age-related resistance to infection 23,24 . Young animals are more susceptible to ARV infections probably because their immune system is not yet fully developed 25 .…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Avian Reovirus Infections in Chickens in Western Provinces of Turkey

Erol¹,

Şengül²

2012

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

show abstract

“…A exemplo do que ocorre em outras infecções onde a patologia é agravada pela co-infecção por agentes imunossupressores, quadros gravíssimos podem ocorrer nos casos de infecção mista envolvendo ARV e/ou MS e o vírus da doença infecciosa da bursa (IBDV) que causa severa imunodepressão nos animais (Moradian et al 1990, Sentíes-Cué et al 2005. A persistência do ARV nos tendões esta relacionada à idade em que ocorre a infecção, permanecendo por um período mais longo quando a infecção ocorre em pintinhos de um dia (Jones & Georgiou 1984).…”

Section: Resultsunclassified

Características clínicas e anatomo-histopatologicas da infecção experimental mista por Orthoreovirus aviario e Mycoplasma synoviae em frangos de corte

et al. 2012

View full text Add to dashboard Cite

Pesq. Vet. Bras. 32(8):687-691, agosto 2012 687 RESUMO.-A artrite infecciosa em frangos de corte representa um problema sanitário e econômico de grande impacto, provocando perdas de produtividade e nos processos de produção e industrialização. Os principais agentes etiológicos associados aos casos de artrites e tenossinovites infecciosas em aves são Mycoplasma synoviae (MS) e Orthoreovirus aviario (ARV). Esse trabalho propôs investigar as alterações anatomohistopatológicas causadas pela infecção experimental concomitante por Mycoplasma synoviae e Orthoreovirus aviario em frangos de corte e conϐirmar a presença dos agentes através das técnicas de PCR e imunoϐluorescência indireta (RIFI Infectious arthritis in broiler represents an economic and health problem resulting in severe losses due to retarded growth and down grading at slaughterhouse. The most common agents associated with cases of infectious arthritis in poultry are Mycoplasma synoviae and Avian orthoreovirus. This study proposed to investigate the histopathological changes caused by mixed infection with Mycoplasma synoviae and Avian orthoreovirus in broilers and conϐirm the presence of the agents through PCR and immunoϐluorescence assay (IFA). We used 16 broiler chickens, housed in bed, with supply of food and water ad libitum. Ten-day-old broilers were infected with Mycoplasma synoviae and Avian orthoreovirus. Clinically, they showed lethargy and swelling of the hock joint. After 30 days, we proceeded to their euthanasia and necropsy. Histological lesions were observed due to the mixed infection with Mycoplasma synoviae and Avian orthoreovirus in different tissues. The histopathology of the joints was characterized by inϐiltration of heterophil leucocytes in the synovial membrane and the digital ϐlexor tendon. The inϐlammatory process was also found in trachea, lungs, air sac, liver, spleen, pericardium and proventriculus. The use of IFA was necessary to verify the presence of both agents in the hock joints, identifying the antigens of Mycoplasma synoviae and Avian orthoreovirus. The presence of M. synoviae was detected by PCR in trachea, lung, air sacs, synovial membrane and synovial ϐluid. Avian orthoreovirus was detected with PCR in liver, spleen, synovial membrane and digital ϐlexor tendon. In conclusion, this investigation suggests that a synergistic relationship exists between Mycoplasma synoviae and Avian orthoreovirus.

show abstract

“…These features make the new challenge model widely applicable. It has been shown that there is an age-related resistance to tenosynovitis caused by avian reovirus infection (Jones & Georgiou, 1984), but that it is therefore important to protect chickens at an early age. Maternal antibodies in progeny from vaccinated breeder flocks offer this protection (Van der Heide et al, 1976;Eidson et al, 1979;Wood & Thornton, 1981;Jones & Nwajei, 1985, Takase et al, 1996aMeanger et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

A reovirus challenge model applicable in commercial broilers after live vaccination

Loon¹,

Suurland²,

Marel³

2002

Avian Pathology

View full text Add to dashboard Cite

The efficacy of live reovirus vaccines may be determined by challenge via the foot pad route 3 to 4 weeks after vaccination. Swelling and discoloration in the foot pad and shank are scored for a period of 14 days. The major disadvantages of this challenge model are the subjective judgement of gross foot pad and/or shank lesions, that it is very difficult to induce lesions in broilers, and that it causes animal suffering. Other reovirus challenge models are based on reisolation of the virus from different tissues or on scoring microscopic lesions in the tendons. Some disadvantages of these models are that they either cannot be used after vaccination with live reovirus because they cannot discriminate between vaccine and challenge virus or that the microscopic lesions scored need not necessarily be related to the challenge virus but may have been induced by other factors. Therefore, we have attempted to develop a reovirus challenge model that was an improvement on the existing ones, using isolation of reovirus from different organs followed by specific detection of the challenge virus with a monoclonal antibody that can discriminate between challenge and vaccine virus. The reovirus challenge model was examined in specific pathogen free (SPF) White Leghorn chickens and commercial broilers. In vivo studies were conducted to examine the efficacy of an attenuated reovirus vaccine in SPF White Leghorn chickens and commercial broilers with maternal immunity against reovirus. No challenge virus could be detected in any of the organs of the vaccinated chickens 3 and 10 days after challenge. In contrast, challenge virus could be isolated from the unvaccinated control group. At an increased challenge dose all unvaccinated challenge control birds were positive, while the vaccinated chickens were protected. It was shown that 1-day-old vaccination in the presence of maternal immunity was effective. It seemed that protection induced in broilers by the attenuated reovirus vaccine may not have been entirely humoral because in protected birds no antibodies against reovirus were detected by enzyme-linked immunosorbent at the time of challenge. Protection in these birds might therefore have been induced by cellular immunity.

show abstract

Reovirus‐induced tenosynovitis in chickens the influence of age at infection

Cited by 57 publications

References 20 publications

Seroprevalence of Avian Reovirus Infections in Chickens in Western Provinces of Turkey

Seroprevalence of Avian Reovirus Infections in Chickens in Western Provinces of Turkey

Características clínicas e anatomo-histopatologicas da infecção experimental mista por Orthoreovirus aviario e Mycoplasma synoviae em frangos de corte

A reovirus challenge model applicable in commercial broilers after live vaccination

Contact Info

Product

Resources

About