Renoprotective effect of the Echinodorus macrophyllus in induced renal injury

Abstract

Objective: Evaluating the renoprotective effect of Echinodorus macrophyllus in acute kidney injury induced by cyclophosphamide in rats. Methods: Experimental research with Wistar rats, male adults, distributed into groups, namely: Controladministration of 1.5 ml sodium chloride 0.9% intraperitoneally; Echinodorous -administration of 2g/kg of Echinodorus macrophyllus by gavage for five days; Cyclophosphamide -administration of cyclophosphamide 150mg/kg intraperitoneally; and Cyclosphosphamide + Echinodorus -administration of Echinodorus macrophyllus and cyclophosphamide. Renal function (creatinine clearance) and the oxidative metabolites (peroxides and urinary substances reactive to thiobarbituric acid, thiols in kidney tissue) were evaluated. Results: Preconditioning with Echinodorus macrophyllus elevated the creatinine clearance and reduced the levels of oxidative metabolites. Conclusion: The antioxidant action of Echinodorus macrophyllus has demonstrated renoprotective effects evidenced by the reduction of oxidative stress in acute renal injury induced by cyclophosphamide in rats. ResumoObjetivo: Avaliar o efeito renoprotetor do Echinodorus macrophyllus na lesão renal aguda induzida pela ciclofosfamida em ratos. Métodos: Pesquisa experimental com ratos Wistar, machos e adultos que foram distribuídos nos grupos: Controle -administração de 1,5 ml de cloreto de sódio a 0,9,% por via intraperitoneal, Echinodorousadministração de 2g/kg de Echinodorus macrophyllus por gavagem durante cinco dias, Ciclofosfamida -administração de ciclofosfamida 150mg/kg por via intraperitoneal, Ciclosfofamida + Echinodorusadministração de Echinodorus macrophyllus e ciclofosfamida. Foram avaliados a função renal (clearance de creatinina) e os metabólitos oxidativos (peróxidos e substâncias reativas ao ácido tiobarbitúrico urinários, tióis no tecido renal). Resultados: O pré-condicionamento com Echinodorus macrophyllus elevou o clearance de creatinina e reduziu os níveis dos metabólitos oxidativos. Conclusão: A ação antioxidante do Echinodorus macrophyllus demonstrou efeito renoprotetor evidenciado pela redução do estresse oxidativo na lesão renal aguda induzida pela ciclofosfamida em ratos.

Phytocompounds from Amazonian Plant Species against Acute Kidney Injury: Potential Nephroprotective Effects

“…Traditionally, the Amazonian population uses extracts from the leaves of E. macrophyllus, from infusion, decoction, or maceration methods, in water or alcohol, to treat urinary system disorders, as they are known to be powerful diuretic agents. In view of popular usage, Nascimento et al [171] demonstrated that preconditioning with E. macrophyllus attenuated cyclophosphamide-induced acute kidney injury in rats as evidenced by increased creatinine clearance and reduced oxidative metabolites in urine and increased reserve of antioxidant enzymes in renal tissue.…”