Renal glucose reabsorption during hypertonic glucose infusion in female streptozotocin-induced diabetic rats

Noonan, William T.; Shapiro, V.M.; Banks, Robert O.

doi:10.1016/s0024-3205(01)01090-6

Cited by 38 publications

(22 citation statements)

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“…Albumin endocytosis by mouse kidney cells visualized by confocal microscopy and fluorescence (Figures 7 and 8) showed no injury or damage. Our results unequivocally confirm the ability of podocytes to reabsorb protein by endocytosis and are in keeping with morphological observations that podocytes have many coated membrane pits and a large number of multivesicular bodies and other endosomal/lysosomal structures within their cell body, suggestive of high endocytic activity and vesicular trafficking [64,65].…”

Section: Discussionsupporting

confidence: 89%

Patho-physiological studies of human blood with kidney dysfunction and therapeutics of albumin functionalized silver nanoparticles in mice kidney cells in vitro

Sironmani¹,

Bhattarai²

2018

Trends Med

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The incidence of acute kidney injury and disease is increasing alarmingly. Inflammatory or nutritional status are associated with kidney disease/ Serum albumin is the marker to identify malnutrition in patients with chronic kidney disease. Treatment with albumin functionalized green synthesized silver nanoparticles help maintain and restore normal cellular function in the kidney. The role of albumin in kidney cell metabolism was studied in-vitro and showed a profound influence of albumin with or without nanoparticles on the level of protein, glucose, and catalase activity. The albumin-nanoparticle treated cells were observed to be healthier as evidenced from the morphology of the cells. Elevated levels of 66 kDa, 59 kDa, 45 kDa, 34, 29 kDa proteins were observed. The proteins may be Heat shock proteins which has cytoprotective properties. The antimicrobial properties of the silver nanoparticles control the infection and inflammation. This novel nanopreparation could lead to the new therapeutics in the treatment of renal injury restoring renal function. Research highlight• Hypoalbuminemia, is highly prevalent in kidney failure and is associated with an increased mortality risk reflecting either the inflammatory or nutritional status of patients• To understand the relationship between malnutrition and serum albumin dosed through functionalized silver nanoparticles the current study was carried out in patients with illness and in organ culture using mice kidney in vitro.• The Albumin functionalized silver nanoparticles were effective combating inflammation and degradation of cellular and extracellular matrix, transporting nutrition to the damaged nephrons prompting self-repair to maintain the status of protein and fat metabolism to avoid dialysis and to support the kidney to work properly preventing acute renal failure.• Besides, the nanoformulation strengthen the immune system to fight chronic problems reducing the oxidative stress and infection preventing acute renal failure.

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Section: Discussionsupporting

confidence: 89%

Patho-physiological studies of human blood with kidney dysfunction and therapeutics of albumin functionalized silver nanoparticles in mice kidney cells in vitro

Sironmani¹,

Bhattarai²

2018

Trends Med

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“…Although this is the first demonstration of down-regulation of organic cation transporters in a diabetes model, adaptations of the facilitative glucose transporters have been demonstrated in STZ-induced diabetes in both functional and molecular studies (Dominguez et al,1994;Chin et al, 1997;Noonan et al, 2001). Goralski et al (2001) reported enhanced transport of the antiviral drug amantadine after 4 days of STZ-induced diabetes via a bicarbonate-dependent transporter the nature of which is yet to be defined.…”

Section: Reduced Roct1 and Roct2 Expression In Diabetes 953mentioning

confidence: 88%

Reduced Expression of Organic Cation Transporters rOCT1 and rOCT2 in Experimental Diabetes

Grover¹,

Buckley²,

Buckley³

et al. 2004

J Pharmacol Exp Ther

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Recent reports have documented a functional deficit of organic cation transport in diabetic rats by an unknown mechanism. This study was designed to test the hypothesis that experimental diabetes decreases expression of organic cation transporters at the basolateral membrane. Streptozotocin-induced diabetic rats were maintained for varying durations after induction of diabetes. A second group of agematched control rats was maintained in a parallel manner. Kinetic analysis of tetraethylammonium accumulation in freshly isolated proximal tubular cells indicated a significantly lower V max value for the diabetics versus controls with no statistical difference in K m values between the two groups. Cortex sections were processed by standard procedures for Northern and immunoblot analysis. Protein expression of the organic cation transporters rOCT1 and rOCT2 progressively decreased with increasing duration of diabetes. After 21 days of diabetes, rOCT1 and rOCT2 were maximally reduced by 50 and 70%, respectively. Quantification of mRNA expression revealed that the roct1 transcript remained unchanged, whereas the roct2 transcript was decreased by 50% after 14 days of diabetes. Treatment with insulin prevented the reductions in transporter levels. These results support the hypothesis by demonstrating that experimental diabetes decreased expression of both rOCT1 and rOCT2 protein and also of roct2 mRNA accumulation. On the other hand, roct1 mRNA levels were unaffected by the diabetic state. This suggests that differences in rOCT2 protein may result from transcriptional and/or translational changes, whereas rOCT1 deficits may be due to posttranscriptional alterations.

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“…Most mechanistic studies of renal glucose transport in diabetes have been performed in animal models (7)(8)(9)(10)(11)(12). The proximal tubular high-capacity and low-affinity SGLT2 plays a key role in renal apical glucose reabsorption (5), and a mutation of the SGLT2 gene is associated with glucosuria (37).…”

Section: Discussionmentioning

confidence: 99%

“…Over the past 2 decades, animal models have contributed enormously to our understanding of the role of epithelial glucose transporters in the renal dysfunction of diabetes (7)(8)(9)(10). SGLT2 is increased in the alloxan-induced diabetic rat (11), and several groups have shown that renal proximal tubular GLUT2 is increased in streptozotocininduced diabetic rats (12)(13).…”

mentioning

confidence: 99%

Glucose Transporters in Human Renal Proximal Tubular Cells Isolated From the Urine of Patients With Non–Insulin-Dependent Diabetes

et al. 2005

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The bulk of glucose that is filtered by the renal glomerulus is reabsorbed by the glucose transporters of the proximal convoluted tubular epithelium. However, it has been difficult to investigate this in diseases such as type 2 diabetes because of the inability to isolate primary renal cells from patients without a renal biopsy. We report here a method for the immunomagnetic isolation and novel primary culture of human exfoliated proximal tubular epithelial cells (HEPTECs) from fresh urine. The primary isolates are highly enriched and differentiated and express characteristic proximal tubular phenotypic markers. They continue to express the proximal tubular markers CD13/aminopeptidase-N, sodium glucose cotransporter (SGLT) 2, and alkaline phosphatase through up to six subsequent subcultures in a similar way to human proximal cells isolated from renal biopsies. In a hyperglycemic environment, HEPTECs isolated from patients with type 2 diabetes expressed significantly more SGLT2 and the facilitative glucose transporter GLUT2 than cells from healthy individuals. We also demonstrated a markedly increased renal glucose uptake in HEPTECs isolated from patients with type 2 diabetes compared with healthy control subjects. Our findings indicate for the first time in a human cellular model that increased renal glucose transporter expression and activity is associated with type 2 diabetes. Diabetes 54:3427-3434, 2005

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Renal glucose reabsorption during hypertonic glucose infusion in female streptozotocin-induced diabetic rats

Cited by 38 publications

References 0 publications

Patho-physiological studies of human blood with kidney dysfunction and therapeutics of albumin functionalized silver nanoparticles in mice kidney cells in vitro

Patho-physiological studies of human blood with kidney dysfunction and therapeutics of albumin functionalized silver nanoparticles in mice kidney cells in vitro

Reduced Expression of Organic Cation Transporters rOCT1 and rOCT2 in Experimental Diabetes

Glucose Transporters in Human Renal Proximal Tubular Cells Isolated From the Urine of Patients With Non–Insulin-Dependent Diabetes

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