Removal of Nonspecifically Bound Proteins on Microarrays Using Surface Acoustic Waves

Cular, Stefan; Branch, Darren W.; Bhethanabotla, Venkat R.; Meyer, Grant D.; Craighead, Harold G.

doi:10.1109/jsen.2008.917478

Cited by 54 publications

(55 citation statements)

References 26 publications

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“…In the former study, 3 ultrasound was applied to samples in test tubes in an ultrasonic water bath, whereas in the latter study, 4 ultrasound was applied to microarrays by SAW.…”

Section: Discussionmentioning

confidence: 99%

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Adaptive Focused Acoustics (AFA) Improves the Performance of Microtiter Plate ELISAs

Green¹,

Rudd

Laugharn

2014

SLAS Discovery

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We investigated the use of Adaptive Focused Acoustics (AFA) technology to improve the performance of microtiter plate enzyme-linked immunosorbent assays (ELISAs). Experiments were performed with commercially available AFA instrumentation and off-the-shelf 96-well microtiter plate sandwich ELISAs. AFA was applied over a range of acoustic energies, temperatures, and durations to the antigen/antibody binding step of an ELISA for measuring HIV-1 p24 in tissue culture samples. AFA-mediated antigen/antibody binding was enhanced up to 2-fold over passive binding at comparable temperatures and was superior or comparable at low temperature (8-10 °C) to passive binding at 37 °C. Lower nonspecific binding (NSB), lower inter-and intra-assay coefficients of variation (CVs), higher Z′ factors, and lower limits of detection (LODs) were measured in AFA-mediated assays compared with conventional passive binding. In a more limited study, AFA enhancement of antigen/antibody binding and lower NSB was measured in an ELISA for measuring IGFBP-3 in human plasma. We conclude from this study that application of AFA to antigen/antibody binding steps in microtiter plate ELISAs can enhance key assay performance parameters, particularly Z′ factors and LODs. These features render AFA-mediated binding assays potentially more useful in applications such as high-throughput screening and in vitro diagnostics than assays processed with conventional passive antigen/antibody binding steps.

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“…In the former study, 3 ultrasound was applied to samples in test tubes in an ultrasonic water bath, whereas in the latter study, 4 ultrasound was applied to microarrays by SAW.…”

Section: Discussionmentioning

confidence: 99%

“…4 SAW is not, however, an acoustic technology that can be readily applied to samples in the wells of a microtiter plate.…”

Section: Research-article2014mentioning

confidence: 99%

Adaptive Focused Acoustics (AFA) Improves the Performance of Microtiter Plate ELISAs

Green¹,

Rudd

Laugharn

2014

SLAS Discovery

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“…Nowadays, these sensors have been applied and used for biological sensor (biosensor) application to detect the DNA and protein [3,4]. This project is important because current biosensor have limitation in the term of low sensitivity and low accuracy in biomolecular detection [5]. For this reason these types of sensor were chosen for general biomedical applications due to its advantages in high sensitivity, ease of integration into electronic circuits, and all around robustness [6,7].…”

Section: Introductionmentioning

confidence: 99%

Hydrothermal growth of titania nanowires for SAW device sensing area

et al. 2017

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Abstract. Synthesis of titania or titanium dioxide (TiO2) is attracted to energy and environmental applications. Here, the growth of nanostructure TiO2 nanowires on Si (100) substrates by using the two-step method. Different seed layers of TiO2 were deposited by spin coating and annealing, followed by the growth of TiO2 nanowires by using the hydrothermal method. The sol-gel technique was used in preparing the TiO2 solution for the thin film deposition purpose. Acetic acid, hydrochloric acid and tris (2-aminoethyl) amine were used as a stabilizer to synthesize three different TiO2 seed layers. The aim of this study was to understand the role of polycrystalline size on thin film towards the diameter of nanowires grown as a sensing area in Surface Acoustic Wave (SAW) Biosensor. The morphology and structure of the thin film and TiO2 nanowires were characterized using X-Ray diffraction (XRD), scanning electron microscope (SEM), field emission scanning electron microscope (FESEM) and atomic force microscopy (AFM).

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“…Nonspecific binding dramatically reduces the sensitivity and selectivity of biosensors [1]. Our previous experimental and numerical studies indicate that the acoustic streaming phenomenon, i.e., fluid motion induced from high intensity sound waves, can be used effectively to remove nonspecifically bound proteins to allow reuse of SAW biosensors (Fig.…”

Section: Introductionmentioning

confidence: 99%

Influence of non-newtonian fluid dynamics on SAW induced acoustic streaming in view of biological applications

Sankaranarayanan

Singh

Bhethanabotla

2011

2011 IEEE SENSORS Proceedings

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Surface acoustic wave (SAW) devices are finding increasing use in medical diagnostic applications, such as detection of specific proteins in bodily fluids for detection of pathologies. These devices can also be used in Lab-On-a-Chip devices for biological applications that utilize micro-fluidics for detection, transport, mixing, and biological assays. In applications aimed at biological sensing, the sensing medium such as blood exhibits a nonNewtonian behavior. In biosensing applications of SAW devices, SAW induced acoustic streaming which refers to fluid motion induced by high frequency sound waves, is an important phenomenon that can be used for the removal of non-specifically bound proteins from the device surface. Acoustic streaming also finds use in a wide variety of other applications such as detection of ovarian cysts and detection of blood clotting via ultrasound and convective transport in microfluidic applications of SAW devices. This work reports on the influence of non-Newtonian fluid dynamics on the acoustic streaming and fluid velocity profiles in SAW devices, using a computational fluid-structure interaction finite element model.

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Removal of Nonspecifically Bound Proteins on Microarrays Using Surface Acoustic Waves

Cited by 54 publications

References 26 publications

Adaptive Focused Acoustics (AFA) Improves the Performance of Microtiter Plate ELISAs

Adaptive Focused Acoustics (AFA) Improves the Performance of Microtiter Plate ELISAs

Hydrothermal growth of titania nanowires for SAW device sensing area

Influence of non-newtonian fluid dynamics on SAW induced acoustic streaming in view of biological applications

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