Removal of escherichia coli from water by systems based on insoluble poly(glycidyl methacrylate)‐G‐tetraethylenepentamine and ‐G‐polyethylenimine

Abstract

A study was made of the removal of Escherichia coli (E. coli) from water by systems based on insoluble poly(glycidy1 methacrylate)-g-tetraethylenepentamine and -gpolyethylenimine, i. e., the reaction products of insoluble poly(glycidy1 methacrylate) (PGMA) beads with tetraethylenepentamine (TEP) or polyethylenimine having molecular weights of about 600 (PEI600). The polymers (PGMA-TEP and PGMA-PEI600) were brought into contact with E. coli in sterilized physiological saline. These polymers made the viable cell numbers decrease in this medium. It was confirmed by observation of polymer surfaces with a scanning electron microscope that this decrease was caused by adsorption of the bacterial cells onto these polymers. The decrease coefficients of viable cell numbers (D) for E. coli by these polymers were 53 ml g-' h-' for PGMA-TEP and 62 ml g-' h-' for PGMA-PEI600, being compared with D by other polymers studied previously. ZUSAMMENFASSUNG:Die Fahigkeit von zwei Polymeren zur Adsorption von Escherichia coli (E. coli) in sterilisierter physiologischer Kochsalzlasung wurde untersucht. Als Polymere wurden die Reaktionsprodukte von unldslichen Poly(glycidylmethacry1at)-Kugelchen (PGMA) mit Tetraethylenpentamin (TEP) bzw. Polyethylenimin mit einem Molekulargewicht von 600 (PEI600) verwendet. Die Zahl der vermehrungsfahigen Zellen nahm in Gegenwart dieser Polymeren ab. Elektronenmikroskopisczh konnte nachgewiesen werden, dal3 diese Abnahme durch Adsorption der Bakterien auf der Polymeroberflache verursacht wird. Die Koeffizienten (D) der Abnahme der vermehrungsfahigen Zellen (E. coli) betragen 53 und 62 ml g-' h-' fur PGMA-TEP bzw. PGMA-PEI600. Sie werden mit den Koeffizienten bereits untersuchter Polymerer verglichen.

Antibacterial and Bacterium Adsorbing Macromolecules

“…Then, PEI600 or TEP was incor-porated to the MR polymer beads (150-300 lm). [95] Their probable structures are shown as 68 ( Figure 43). Since the beads were so hard and scarcely swell in solvents, the amounts of reacted PEI600 and TEP were small, particularly gel type beads reacted scarcely with TEP or PEI600.…”

Synthesis and antibacterial activity of immobilized quaternary ammonium salts

“…So far, insoluble carriers such as cellulose, starch, agarose, porous glass, and cross-linked styrene/divinylbenzene have been used as carriers for the immobilization of antibiotics, l6,I7 metallorganic compounds, 7*8 and quaternary ammonium salts?, [18][19][20][21] In addition, the antimicrobial activity of cross-linked 4-vinyl alkylpyridinium salts has been reported, 22 but later investigations demonstrated that this effect was due to adsorption of cells to the surface.23 Recently, insoluble polymer beads based on crosslinked polystyrene-polyethyleneimine polymers, 24,25 on poly ( glycidyl methacrylate ) -tetraethylenepentamine, 26 and on activated alumina-supported poly ( styrene-co-chloromethylstyrene ) 27 were investigated for the removal of bacteria from water. A decrease of viable cell numbers was observed, due to the adsorption of cells to the surface of these polymers.…”

Preparation of resins having various phosphonium groups and their adsorption and elution behavior for anionic surfactants

“…Recently, insolubilized agents having antibacterial activity have been developed 1–7. Insolubilized agents having positively charged groups, such as quaternary ammonium groups and phosphonium groups, or triethylenetetramine side chains, thiol groups, and phenol derivatives are reported to exhibit high antibacterial activity 8–12.…”