Remote nongenetic optical modulation of neuronal activity using fuzzy graphene

Rastogi, Sahil Kumar; Garg, Raghav; Scopelliti, Matteo Giuseppe; Pinto, Bernardo I.; Hartung, J; Kim, Seokhyoung; Murphey, Corban; Johnson, Nicholas; Roman, Daniel San; Bezanilla, Francisco; Cahoon, James F.; Gold, Michael S.; Chamanzar, Maysamreza; Cohen‐Karni, Tzahi

doi:10.1073/pnas.1919921117

Cited by 66 publications

(92 citation statements)

References 65 publications

(111 reference statements)

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“…Although these results suggest dominant photothermal phenomenon in 3DFG, we have identified key distinguishing factors that might be responsible for the observed faradaic response in the current study. The peak power of the laser used during the picosecond-long laser pulse for the photocurrent measurements ranges from 1.5 to 11 W. This range of laser power values are orders of magnitude higher than that reported in the previous work about NT-3DFG ( 26 ). Furthermore, the spot size of the laser used in this work is smaller than that used in the previous study (2 μm versus 20 μm, respectively) ( 26 ).…”

Section: Resultsmentioning

confidence: 65%

“…In a previous work, nanowire-templated 3DFG (NT-3DFG) was shown to exhibit high photothermal response under low-power laser illumination ( 26 ), ascribable to an opto-capacitive rather than faradaic regime ( 27 ). Although these results suggest dominant photothermal phenomenon in 3DFG, we have identified key distinguishing factors that might be responsible for the observed faradaic response in the current study.…”

Section: Resultsmentioning

confidence: 99%

“…These bioelectronics could provide fundamental features such as high mechanical flexibility for in vivo applications, high biocompatibility for long-term experiments, low cost for pharmaceutical screening applications, and absence of noble metals or polluting materials for environmental considerations. Future efforts will include intracellular recording stabilizations via templated growth from wires or tubes ( 18 , 45 , 46 ) or surface chemical modifications ( 26 , 47 ).…”

Section: Resultsmentioning

confidence: 99%

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Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes

et al. 2021

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Graphene with its unique electrical properties is a promising candidate for carbon-based biosensors such as microelectrodes and field effect transistors. Recently, graphene biosensors were successfully used for extracellular recording of action potentials in electrogenic cells; however, intracellular recordings remain beyond their current capabilities because of the lack of an efficient cell poration method. Here, we present a microelectrode platform consisting of out-of-plane grown three-dimensional fuzzy graphene (3DFG) that enables recording of intracellular cardiac action potentials with high signal-to-noise ratio. We exploit the generation of hot carriers by ultrafast pulsed laser for porating the cell membrane and creating an intimate contact between the 3DFG electrodes and the intracellular domain. This approach enables us to detect the effects of drugs on the action potential shape of human-derived cardiomyocytes. The 3DFG electrodes combined with laser poration may be used for all-carbon intracellular microelectrode arrays to allow monitoring of the cellular electrophysiological state.

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Section: Resultsmentioning

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes

et al. 2021

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“…Therefore, the photothermal effect from indirect bandgap semiconductors, such as silicon materials, can be used to for cell modulation. [41] For some nanostructures, such as gold nanorod [42] and carbon nanomaterials, [76,77] the surface plasmon effect can convert almost all absorbed energy to heat, creating a strong temperature gradient for photothermal biological modulation.…”

Section: Connection To Photothermal Processesmentioning

confidence: 99%

“…Photo thermal stimulation of DRG cells at subcellular spatial resolution with laser energy one or two orders of magnitude lower than other platforms, such as gold or silicon, was achieved, because of the high photothermal efficiency and subcellular dimensions of the nanowire graphene composite (Figure 6f). [77] Gold displays good biocompatibility and high optical responses and it experiences heating upon illumination with NIR light through the plasmonic effect. [145,146] The surface of gold can be easily modified with ligands and antibody for highspecificity interfacing; therefore, gold nanomaterials are a good candidate for NIR photothermal neural stimulation.…”

Section: Optocapacitive Effects For Modulationmentioning

confidence: 99%

Nano‐ and Microscale Optical and Electrical Biointerfaces and Their Relevance to Energy Research

Hou

Yang

Shi

et al. 2021

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attributes of both biotic and abiotic materials for unprecedented opportunities in life and energy sciences. [10] Recent developments in micro-and nano-technology have yielded biointerfaces on multiple length scales, ranging from the nanoscale subcellular level to the macroscale level of epidermal electronics. Freestanding nanostructures and nanoscale devices allow cellular and subcellular biointerfaces, while macroscale devices enable the formation of biointerfaces with tissues and organs. Furthermore, a range of nano-and micro-fabrication methods for 3D structures has been developed, including 3D printing, [12][13][14][15][16][17][18] colloidal selfassembly, [19,20] controlled folding, [21][22][23] and templated growth. [24][25][26] Early progress in bioelectronics enabled integrating electronic elements with cellular scaffolds for monitoring and control of tissue functions and activities in a 2D configuration. However, biological tissues inherently adopt a complex 3D architecture, in which cells inhabit a complex microenvironment comprising extracellular matrix (ECM) and neighboring cells. 3D materials with defined geometries, compatible mechanical properties, and integrated electronic components represent an ideal scaffold for monitoring and regulating cell behaviors in the 3D microenvironment. Indeed, 3D biointerfaces are now advancing our understanding of molecular mechanisms involved in cancer. [1,27,28] They are also creating new opportunities in the fields of tissue engineering and biomedicine, where 3D constructs grown in vitro or ex vivo can then be transplanted into the human body for organ repair. [29] In this review, we summarize recent progress in nano-and microscale optical and electrical biointerfaces, where nanostructured materials and microfabricated systems are used to interface with biological systems. Based on the fundamental science inherent to the device/material biointerface, we first discuss the connection between energy science and bioelectronics. Then, we give an overview of bioelectronics tools for biointerfaces. Further, we focus on representative biointerfaces, including neural, cardiac, and microbial biointerfaces. Finally, we discuss future opportunities for nano-and microscale bioelectronics. The Connection between Biointerfaces and Energy SciencesBiological systems communicate with and respond to the external world via different cues, such as electrical, chemical, Different research fields in energy sciences, such as photovoltaics for solar energy conversion, supercapacitors for energy storage, electrocatalysis for clean energy conversion technologies, and materials-bacterial hybrid for CO 2 fixation have been under intense investigations over the past decade. In recent years, new platforms for biointerface designs have emerged from the energy conversion and storage principles. This paper reviews recent advances in nano-and microscale materials/devices for optical and electrical biointerfaces. First, a connection is drawn between biointerfaces and energy science, and how these t...

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Semiconductor Nanowire‐Based Cellular and Subcellular Interfaces

Shi

Sun

Liang

et al. 2021

Adv Funct Materials

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The highly intricate structures of biological systems make the precise probing of biological behaviors at the cellular‐level particularly difficult. As an advanced toolset capable of exploring diverse biointerfaces, high‐aspect‐ratio nanowires stand out with their unique mechanical, optical, and electrical properties. Specifically, semiconductor nanowires show much promise in their tunability and feasibility for synthesis and fabrication. Thus far, semiconductor nanowires have shown favorable results in deciphering biological communications and translating this cellular language through the nanowire‐based biointerfaces. In this perspective, the synthesis and fabrication methods for different kinds of nanowires and nanowire‐based structures are first surveyed. Next, several cellular‐level nanowire‐enabled applications in biophysical dynamics probing, physiological or biochemical sensing, and biological activity modulation are highlighted. Then, the progress of functionalized nanowires in drug delivery and bioenergy production is reviewed. Finally, the current limitations of nanowires and an outlook into the next generation of nanowire‐based devices at the biointerfaces are concluded.

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Remote nongenetic optical modulation of neuronal activity using fuzzy graphene

Cited by 66 publications

References 65 publications

Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes

Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes

Nano‐ and Microscale Optical and Electrical Biointerfaces and Their Relevance to Energy Research

Semiconductor Nanowire‐Based Cellular and Subcellular Interfaces

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